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pcDNA3-PB1-B/BRISBANE/60/2008

Plasmid

#187919

Purpose

Expresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cells

Depositor

Arindam Mondal

Article

Kedia et al Front Microbiol. 2022 May 25;13:868367.

Insert

polymerase basic protein 1

Tags

UNTAGGED

Promoter

CMV Promoter

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

Plasmid

#84246

Purpose

Expresses direct fusion VPR-Sa dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sa dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEBTet-β-tubulin_SNAP

Plasmid

#136831

Purpose

Mammalian expression of the centrosomal protein _-tubulin C-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

TUBB_SNAP (TUBB Synthetic, Human)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP HSPH1

Plasmid

#19480

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

HSPH1 (HSPH1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag TSC2 (S939A/T1462A)

Plasmid

#14131

Depositor

Brendan Manning

Article

Manning et al Mol Cell. 2002 Jul . 10(1):151-62.

Insert

TSC2 S939A/T1462A (TSC2 Human)

Tags

Flag

Expression

Mammalian

Mutation

S939A/T1462A

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only
pSLC-254

Plasmid

#124291

Purpose

Expression of vsfGFP-0. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a dimeric fluorophore.

Depositor

Swaine Chen

Article

Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4

Insert

vsfGFP-0

Expression

Bacterial

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-IF2

Plasmid

#224367

Purpose

Express IF2 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

IF2 (infB E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-RF3-His6

Plasmid

#224373

Purpose

Express RF3 protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

RF3 (prfB E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-His6-AsnRS

Plasmid

#224363

Purpose

Express AsnRS protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

AsnRS (asnS E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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PT7lacO-RRF-His6

Plasmid

#224374

Purpose

Express RRF protein for his-tagged purification

Depositor

Richard Murray

Article

Zhang et al ACS Synth Biol. 2025 May 16;14(5):1496-1

Insert

RRF (frr E. coli)

Tags

His6

Expression

Bacterial

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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