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Showing: 7661 - 7670 of 9121 results

  1. pLM494

    Plasmid
    #100539
    Purpose
    CEN/ARS plasmid harboring expression cassettes for crtI, crtYB, crtE and tHMG1 of the β-carotene pathway, each flanked by loxPsym sites.
    Depositor
    Bernd Müller-Röber
    Article
    Hochrein et al Nat Commun. 2018 May 22;9(1):1931. doi:
    Inserts
    GGPP synthase
    phytoene desaturase
    phytoene-beta carotene synthase
    HMG CoA reductase
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Yeast
    Promoter
    PGK1, TDH3, TIP1, and ZEO1
    Available Since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    100539-plasmid-map-sequence-id-194476

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  2. Cre Reporter

    Plasmid
    #62732
    Purpose
    Lentiviral Cre recombinase reporter; EF1a-loxP-DsRedExpress2-loxP-EGFP. Cre excises the loxP-flanked RFP-STOP cassette and switches fluorescence from red (RFP) to green (GFP).
    Depositor
    Niels Geijsen
    Article
    D'Astolfo et al Cell. 2015 Apr 23;161(3):674-690. doi: 1
    Insert
    LoxP-DsRED-STOP-LoxP-eGFP
    Use
    Cre/Lox and Lentiviral
    Tags
    LoxP sites
    Expression
    Mammalian
    Promoter
    EF1-Alpha
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    62732-plasmid-map-sequence-id-204219

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  3. pOCC175

    Plasmid
    #118890
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal monomeric GFP, cleavable with TEV
    Depositor
    Marc Bickle, PEPC MPI CBG
    Article
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-HIS6-MBP-3C-mGFP-TEV-NotI-ccdB-AscI-stop-HindIII cassette
    Tags
    HIS6-MBP, cleavable with 3C protease; mGFP, cleav…
    Expression
    Insect
    Promoter
    polH
    Available Since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118890-plasmid-map-sequence-id-232288

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  4. Equalizer-L episome

    Plasmid
    #169738
    Purpose
    Equalizer-L on an episomal vector. This plasmid encodes eGFP to report circuit output levels. This plasmid also encodes a separate mCherry expression cassette to monitor plasmid dosage.
    Depositor
    Francois St-Pierre
    Article
    Yang et al Nat Commun. 2021 Jul 5;12(1):4132. doi:
    Insert
    miR(FF4) target-tetR-P2A-eGFP-miR(FF4) target-miR(FF4)
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    pCMV-tetO2
    Available Since
    Oct. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    169738-plasmid-map-sequence-id-334158

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  6. gRNA-CasRx_SD1-pSV40-TagBFP

    Plasmid
    #221004
    Purpose
    Transiently express CasRx DR30 repeat with gRNA spacer. Contains SD1 mutation to enhance efficiency. SV40-TagBFP cassette to monitor transfection efficiency. Use BbsI with overhangs Fw-AAAC, Rv-AAAA.
    Depositor
    Benjamin Blencowe, Mikko Taipale
    Article
    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e
    Insert
    CasRx 30nt processed direct repeat with SD1 mutation
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    DR1 A>T
    Promoter
    U6
    Available Since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    221004-plasmid-map-sequence-id-436033

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  10. MF85_PB-14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA

    Plasmid
    #176629
    Purpose
    TF C self-activation cassette in MultiFate-3
    Depositor
    Michael Elowitz
    Article
    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.
    Insert
    14xUAS-12x42bs-10x(ErbB2bs_ErbB2bs)-miniCMV-NLS-FKBP12F36V-ErbB2ZFR2AR39A-VP16-NLS-DHFR-IRES-mTurquoise2-PEST-BGHpA
    Use
    Synthetic Biology
    Available Since
    Jan. 27, 2022
    Availability
    Academic Institutions and Nonprofits only
    176629-plasmid-map-sequence-id-347269

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Showing: 7661 - 7670 of 9121 results