We narrowed to 2,364 results for: PUC19

1. pAT9748-dCBE

   Plasmid

   #163008

   Purpose

   plasmid expressing CBE with dCas9

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   dCBE
   

   Use

   CRISPR

   Tags

   3xFLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   EF1-a

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDAS12342_PEAR-GFP_2in1

   Plasmid

   #177184

   Purpose

   PEAR-GFP plasmid with a pegRNA that targets its own plasmid

   Depositor

   Ervin Welker

   Article

   Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

   Inserts

   EGFP split with an intron between amino acids 95-96
   pegRNA targeting its own plasmid
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   disrupted 5' splice site

   Promoter

   CMV and U6

   Available Since

   Feb. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas34

   Plasmid

   #82386

   Purpose

   sgRNA targeting YFP expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistance

   Depositor

   Brian Pfleger

   Article

   Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

   Insert

   gRNA
   

   Promoter

   PEZ3

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1071-Lenti-Single loxP

   Plasmid

   #217892

   Purpose

   Lentiviral vector, entry vector for Lentiviral Switch-OVER

   Depositor

   Ulrich Elling

   Article

   Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

   Type

   Empty backbone

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. qTAG-AAVS1-Ef1a-Zeo

   Plasmid

   #227268

   Purpose

   Empty AAVS1 targeting donor for the insertion of Zeo and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Type

   Empty backbone

   Use

   CRISPR; Donor cassette

   Expression

   Mammalian

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT9658-sgRNA-mCherry

   Plasmid

   #162987

   Purpose

   sgRNA cloning plasmid

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   exchangeable cassette
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   U6

   Available Since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILGFPE9

   Plasmid

   #83545

   Purpose

   Used to evaluate the expression output of TEF1 promoter with yEGFP used as the reporter gene.

   Depositor

   Claudia Vickers

   Article

   Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

   Insert

   TEF1 promoter-yEGFP
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   Oct. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLinker-AID-3xHA-HXGPRT-LoxP

   Plasmid

   #86553

   Purpose

   used for a TIR1-AID system with CRISPR tagging technology in Toxoplasma gondii

   Depositor

   David Sibley

   Article

   Long et al PLoS Pathog. 2017 May 5;13(5):e1006379.

   Insert

   Linker-AID-3xHA-HXGPRT 3'UTR
   

   Use

   Expression in toxoplasma gondii

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSLQ5135: pCAG-BFP-GuideArray

   Plasmid

   #210606

   Purpose

   Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

   Depositor

   Stanley Qi

   Article

   Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

   Insert

   pCAG-BFP-GuideArray
   

   Use

   CRISPR

   Promoter

   CAG

   Available Since

   Dec. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pL0-MtPT4Pro

    Plasmid

    #159414

    Purpose

    Medicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtPT4 Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pL0-MtBCP1Pro

    Plasmid

    #159415

    Purpose

    Medicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.

    Depositor

    Sam Brockington

    Article

    Timoneda et al PLoS Biol. 2021 Jul 14;19(7):e3001326. d

    Insert

    MtBCP1 Promoter
    

    Use

    Puc19-derived

    Expression

    Bacterial

    Available Since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDM098

    Plasmid

    #216818

    Purpose

    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Insert

    mStayGold
    

    Tags

    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3

    Expression

    Bacterial

    Mutation

    Aspergillus nidulans codon-adjusted

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJAID5XY

    Plasmid

    #165051

    Purpose

    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

    Depositor

    Claudia Vickers

    Article

    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

    Insert

    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    

    Expression

    Yeast

    Mutation

    OsTIR1 is codon-optimised for expression in S. ce…

    Available Since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pShoHELIX-sgRNA_entry (BO3)

    Plasmid

    #181784

    Purpose

    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    

    Expression

    Bacterial

    Mutation

    nAniI = K227M, F80K, L232K

    Promoter

    Lac and J23119

    Available Since

    March 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pILGFP95

    Plasmid

    #83550

    Purpose

    Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    PDA1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    PDA1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pTX1-SMN1-exon7-ESE1

    Plasmid

    #126041

    Purpose

    In-vitro-transcription of SMN1-exon7-ESE1 RNA in NMR tube for "Systems NMR" analysis

    Depositor

    Frédéric Allain

    Article

    Nikolaev et al Nat Methods. 2019 Aug;16(8):743-749. doi

    Insert

    SMN1 exon7 ESE1
    

    Available Since

    May 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pTetQCas-8+IS186

    Plasmid

    #170636

    Purpose

    Expression of CRISPR-associated transposases

    Depositor

    Sheng Yang

    Article

    Zhang et al CRISPR J. 2021 Jun;4(3):350-359. doi: 10

    Insert

    VchTniQ, VchCas8, VchCas7, VchCas6, CRISPR(8+IS186 array)
    

    Expression

    Bacterial

    Available Since

    Nov. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pILGFPH7

    Plasmid

    #83554

    Purpose

    Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    ADH2 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    ADH2

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pILGFP97

    Plasmid

    #83551

    Purpose

    Used to evaluate the expression output of CYC1 promoter with yEGFP used as the reporter gene.

    Depositor

    Claudia Vickers

    Article

    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

    Insert

    CYC1 promoter-yEGFP
    

    Expression

    Yeast

    Promoter

    CYC1

    Available Since

    Dec. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAL1

    Plasmid

    #197285

    Purpose

    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195

    Depositor

    Bogumil Karas

    Article

    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.

    Insert

    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Available Since

    April 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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