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We narrowed to 2,346 results for: PUC19

Showing: 1521 - 1540 of 2346 results

  2. pDM098

    Plasmid
    #216818
    Purpose
    Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for C-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mStayGold
    Use
    Tags
    FLAG-(SGGS)x2-XTEN16-(GGGGS)x3
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Promoter
    Available since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216818-plasmid-map-sequence-id-429609

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  3. pJAID5XY

    Plasmid
    #165051
    Purpose
    Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.
    Depositor
    Claudia Vickers
    Article
    Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:
    Insert
    P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    OsTIR1 is codon-optimised for expression in S. ce…
    Promoter
    Available since
    March 12, 2021
    Availability
    Academic Institutions and Nonprofits only
    165051-plasmid-map-sequence-id-327149

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  4. pILGFP95

    Plasmid
    #83550
    Purpose
    Used to evaluate the expression output of PDA1 promoter with yEGFP used as the reporter gene.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Insert
    PDA1 promoter-yEGFP
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    PDA1
    Available since
    Dec. 15, 2016
    Availability
    Academic Institutions and Nonprofits only

  5. pShoHELIX-sgRNA_entry (BO3)

    Plasmid
    #181784
    Purpose
    Expresses ShoHELIX containing a nicking I-AniI fusion to ShoTnsB. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
    Use
    Tags
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    181784-plasmid-map-sequence-id-358224

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  11. pAL1

    Plasmid
    #197285
    Purpose
    Shuttle plasmid that replicates in E. coli, S. cerevisiae, and A. laidlawii. Contains the OriC from A. laidlawii 8195
    Depositor
    Bogumil Karas
    Article
    Nucifora et al ACS Synth Biol. 2024 Jan 19;13(1):45-53.
    Insert
    Origin of replication of A. laidlawii 8195: whole dnaA gene, plus upstream and downstream intergenic regions
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    197285-plasmid-map-sequence-id-389699

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  16. pMG

    Plasmid
    #138264
    Purpose
    Yeast intergration vector for integration of dual reporter system at the URA3 locus. Dual reporter system contains constitutive mCherry and constitutive eGFP flanked by iCas9 sites.
    Depositor
    Xiao Wang
    Article
    Standage-Beier et al CRISPR J. 2019 Aug;2:209-222. doi: 10.10
    Inserts
    eGFP flanked by iCas9 sites
    mCherry
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tef1
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    138264-plasmid-map-sequence-id-273626

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  17. pITGFP89

    Plasmid
    #83560
    Purpose
    Used to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.
    Depositor
    Claudia Vickers
    Article
    Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi
    Insert
    TEF1 promoter -yEGFP-CLN2PEST
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    TEF1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83560-plasmid-map-sequence-id-159500

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  20. pILGFP9E3

    Plasmid
    #185842
    Purpose
    Testing the TEF1 promoter inserted with 4 TetO elements using a green fluorescence protein as the reporter
    Depositor
    Claudia Vickers
    Article
    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:
    Insert
    PURA3>KlURA3>TAgTEF1-PTEF1+[TetO]>yEGFP>TPGK1-TURA3
    Use
    Tags
    Expression
    Yeast
    Mutation
    None
    Promoter
    Available since
    July 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    185842-plasmid-map-sequence-id-368107

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Showing: 1521 - 1540 of 2346 results