We narrowed to 6,952 results for: crispr cas9 plasmids

1. pITI1

   Plasmid

   #166094

   Purpose

   Gal inducible expression of dCas9-FokI

   Depositor

   Rebecca Burgess

   Insert

   dCas9-FokI
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   spCas9-D10A, H840A

   Promoter

   Gal-inducible

   Available since

   April 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pXZ13lacZalpha

   Plasmid

   #160229

   Purpose

   places LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal plates

   Depositor

   Ian Duncan

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   lac

   Available since

   Oct. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCM4.4

   Plasmid

   #217648

   Purpose

   an ermE*p-cas9-driven expression, as an alternative plasmid

   Depositor

   Huimin Zhao

   Article

   Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

   Insert

   Codon Optimized SpCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMZ374

   Plasmid

   #59896

   Purpose

   Combination adh1:cas9/rrk1:sgRNA for CRISPR genome editing in fission yeast: Empty sgRNA target (CspCI placeholder) (see comments).

   Depositor

   Mikel Zaratiegui

   Article

   Jacobs et al Nat Commun. 2014 Oct 29;5:5344. doi: 10.

   Inserts

   Cas9
   rrk1:sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Silent muation of the CspCI site

   Promoter

   adh1 and rrk1

   Available since

   Oct. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAH237

   Plasmid

   #121146

   Purpose

   Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast

   Depositor

   Katsunori Tanaka

   Article

   Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

   Inserts

   gRNA cassette
   humanized Streptococcus pyogenes Cas9
   

   Use

   CRISPR

   Tags

   Flag

   Expression

   Yeast

   Mutation

   Promoter

   S.pombe rrk1 and nmt41

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIO-GFP

   Plasmid

   #74119

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and EGFP-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AIO-mCherry

   Plasmid

   #74120

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGMC00018 (aka pRC0082)

   Plasmid

   #195320

   Purpose

   Minimal plasmid backbone with multiple cloning site for HDR donor DNA construction

   Depositor

   Raj Chari

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti SpBsmBI sgRNA Hygro

   Plasmid

   #62205

   Purpose

   An empty sgRNA expression vector for expression of sgRNA for Sp Cas9

   Depositor

   Rene Maehr

   Article

   Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti SpBsmBI sgRNA Puro

    Plasmid

    #62207

    Purpose

    An empty sgRNA expression vector for expression of sgRNA for Sp Cas9 (3rd generation lentiviral vector)

    Depositor

    Rene Maehr

    Article

    Pham et al Methods Mol Biol. 2016;1358:43-57. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pJB166

    Plasmid

    #86998

    Purpose

    CRISPR/Cas9 in fission yeast using fluoride selection

    Depositor

    Julien Berro

    Article

    Fernandez et al Yeast. 2016 Oct;33(10):549-557. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Feb. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJAT74

    Plasmid

    #204296

    Purpose

    pJAT plasmid with multiple cloning site for introducing custom payload. One U6 promoter.

    Depositor

    David Stern

    Article

    Stern et al bioRxiv 2023.06.17.545412

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX330 Mouse 3' Hp1a gRNA

    Plasmid

    #127898

    Purpose

    WT Cas9 Vector targeting the 3' end of the mouse Hp1a gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX330 Mouse 5' Npm1 gRNA

    Plasmid

    #127900

    Purpose

    WT Cas9 Vector targeting the 5' end of the mouse Npm1 gene

    Depositor

    Mark Groudine

    Insert

    gRNA/Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. px330_SMAD exon 9 gRNA

    Plasmid

    #68352

    Purpose

    px330 with gRNA towards SMAD exon 9. Cas9 is expressed from a CAG promoter.

    Depositor

    Jannik Elverløv-Jakobsen

    Insert

    SMAD exon 9 gRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAW016-2

    Plasmid

    #85613

    Purpose

    Cas9-tracrRNA integration vector for Bacillus subtilis

    Depositor

    Perry Chou

    Article

    Westbrook et al Appl Environ Microbiol. 2016 Jun 3. pii:

    Insert

    cas9-tracrRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAH243

    Plasmid

    #121147

    Purpose

    Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast

    Depositor

    Katsunori Tanaka

    Article

    Hayashi et al G3 (Bethesda). 2019 Feb 12. pii: g3.118.

    Inserts

    humanized Streptococcus pyogenes Cas9
    gRNA expression module
    

    Use

    CRISPR

    Tags

    Flag

    Expression

    Yeast

    Mutation

    Promoter

    S.pombe rrk1 and nmt41

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. MSP1673

    Plasmid

    #65769

    Purpose

    Bacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
    

    Use

    CRISPR

    Tags

    NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. px330-mcherry

    Plasmid

    #98750

    Purpose

    Cas9 from S.pyogenes with CMV-mcherry cassette, and cloning backbone for sgRNA

    Depositor

    Jinsong Li

    Article

    Wu et al Cell Stem Cell. 2013 Dec 5;13(6):659-62.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBLO1811_arC9_noNLS_human

    Plasmid

    #74494

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    CRISPR

    Tags

    t2a mCherry (C-terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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