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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,072 results for: golden gate

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Showing: 1521 - 1540 of 4072 results

  1. L1T18 - T2PsB/p35S preswitched (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219690
    Purpose
    Level 1 preswitched History-dependent target, switch with Bxb1, p35S promoter, and output: mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PsB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219690-plasmid-map-sequence-id-435857

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  2. L1T11 - T2BP/p35S (BFP, mCherry, NeonGreen)

    Plasmid
    #219688
    Purpose
    Level 1 History-dependent target, switch Bxb1 then PhiC31, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2BP/p35S (BFP, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219688-plasmid-map-sequence-id-435723

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  3. L1T9 - T2PB/p35S (mTagBFP2, mCherry, NeonGreen)

    Plasmid
    #219686
    Purpose
    Level 1 History-dependent target, switch PhiC31 then Bxb1, p35S promoter, and output: mtagBFP2 then mCherry then NeonGreen (for transformation, Kan plant resistance, Kan bacteria resistance).
    Depositor
    Jennifer Nemhauser
    Insert
    T2PB/p35S (mTagBFP2, mCherry, NeonGreen)
    Expression
    Plant
    Available Since
    July 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    219686-plasmid-map-sequence-id-436048

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  4. MUT_S7-H1

    Plasmid
    #218396
    Purpose
    3-exon minigene, with SRSF7 motif-rich middle exon, hnRNPA1 motif-rich region in second intron, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    May 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218396-plasmid-map-sequence-id-433914

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  5. IBMc233

    Plasmid
    #161939
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. PPhlF as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-T-cat-PPhlF-RiboJ-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    PPhlF
    Available Since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161939-plasmid-map-sequence-id-327549

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  6. IBMc050

    Plasmid
    #161938
    Purpose
    Carries Golden Gate substitution insert for IBM with M86 intein. Plux2 as C-lobe promoter
    Depositor
    Baojun Wang
    Article
    Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:
    Insert
    pSB1A3-BbsI-M86N-cat-Plux2-B32-M86C-SapI
    Expression
    Bacterial
    Promoter
    Plux2
    Available Since
    March 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    161938-plasmid-map-sequence-id-327548

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  9. pRDB_248

    Plasmid
    #245335
    Purpose
    Cas12a CRISPRko positive control guide; targets CD63
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD63 (CD63 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245335-plasmid-map-sequence-id-488021

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  10. pAGM4723:Ble:Cas9

    Plasmid
    #112208
    Purpose
    Golden Gate Level 2 construct encoding Cas9-YFP, Zeocin resistance.
    Depositor
    Thomas Mock
    Inserts
    Cas9
    SheBle
    Use
    CRISPR; Diatom, phaeodactylum
    Tags
    Phaeodactylum fcp promoter and terminator and YFP
    Mutation
    Phaeodactylum Fcp promoter
    Promoter
    Phaeodactylum Fcp
    Available Since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    112208-plasmid-map-sequence-id-217248

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  13. pMV_AA101

    Plasmid
    #245323
    Purpose
    Cas9 CRISPRa positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245323-plasmid-map-sequence-id-488047

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  14. pRDB_510

    Plasmid
    #245337
    Purpose
    Cas9 CBE positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245337-plasmid-map-sequence-id-488041

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  15. pRDB_509

    Plasmid
    #245336
    Purpose
    Cas9 ABE positive control guide; targets CD274
    Depositor
    John Doench, David Root
    Insert
    sgRNA CD274 (CD274 Synthetic)
    Use
    Lentiviral
    Mutation
    None
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    245336-plasmid-map-sequence-id-488033

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  16. pTwist-Kan-HC-L0-TaMOFB1(int)

    Plasmid
    #242005
    Purpose
    L0 CDS1 Golden Gate part of the pre-mRNA (excluding UTRs) of Triticum aestivum MORE FLORET 1 (MOF-1) / MULTI-FLORET SPIKELET 2 (MFS-2) B-subgenome homoeolog (TraesCS2B02G420900)
    Depositor
    Cristobal Uauy
    Insert
    TaMOF-B1_with_introns
    Use
    Synthetic Biology
    Expression
    Plant
    Available Since
    Oct. 2, 2025
    Availability
    Academic Institutions and Nonprofits only
    242005-plasmid-map-sequence-id-487883

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  17. pAIP4_3CX2

    Plasmid
    #234221
    Purpose
    Heterologous protein expression of myosin-binding protein C, cardiac-type in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    3CX2 (MYBPC3 Human)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234221-plasmid-map-sequence-id-464186

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  18. pAIP4_4ACJ

    Plasmid
    #234212
    Purpose
    Heterologous protein expression of Wufb25h12 in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    4ACJ (oxr1a Zebrafish)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234212-plasmid-map-sequence-id-464177

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  19. pAIP4_1EYH

    Plasmid
    #234159
    Purpose
    Heterologous protein expression of epsin-1 (EPS-15-interacting protein 1) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1EYH (Epn1 Rat)
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234159-plasmid-map-sequence-id-464125

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  20. MUT_S1-spacer-H1

    Plasmid
    #218399
    Purpose
    3-exon minigene, with SRSF1 motif-rich middle exon, hnRNPA1 motif-rich region in second intron following a neutral spacer, and AG>GG mutation in final 3' splice site
    Depositor
    Oded Regev
    Insert
    Modified CHO DHFR minigene
    Expression
    Mammalian
    Promoter
    tetracycline-responsive promoter
    Available Since
    June 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    218399-plasmid-map-sequence-id-436404

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Showing: 1521 - 1540 of 4072 results