We narrowed to 10,856 results for: kars;

1. TET-pLKO.1 PURO shId2 #2

   Plasmid

   #83090

   Purpose

   Lentiviral shRNA vector for inducible knockdown of mouse Id2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shId2 (Id2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA-Flag-EcoCas6

   Plasmid

   #106274

   Purpose

   Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasE
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-LmoCas6-KRAB-2a-BlastR

   Plasmid

   #126492

   Purpose

   Encodes lentivirus expression of L. monocytogenes CRISPR Type I-B Flag-Cas6-KRAB-2a-BlastR driven by hUbC promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   LmoCas6-KRAB-2a-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hUbC

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-Flag-EcoCas8e

   Plasmid

   #106270

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasA with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasA
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-Flag-EcoCse2

   Plasmid

   #106271

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasB with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasB
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-Flag-EcoCas7

   Plasmid

   #106272

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasC with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasC
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA-Flag-EcoCas5

   Plasmid

   #106273

   Purpose

   Encodes human codon optimized E.coli CRISPR Type I-E CasD with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasD
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3.1-myc-BirA*-NRASG12V

   Plasmid

   #122209

   Purpose

   Expresses myc-BirA* fused to NRASG12V in mammalian cells

   Depositor

   Christopher Counter

   Article

   Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

   Insert

   NRASG12v (NRAS Human)
   

   Use

   Tags

   myc-BirA*

   Expression

   Mammalian

   Mutation

   changed G12 to V

   Promoter

   CMV

   Available since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. TET-pLKO.1 PURO shId2 #1

   Plasmid

   #83088

   Purpose

   Lentiviral shRNA vector for inducible knockdown of mouse Id2

   Depositor

   Kevin Janes

   Article

   Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

   Insert

   shId2 (Id2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

10. pSC5-toxic3

    Plasmid

    #188625

    Purpose

    pSC5GGv2 with HindII Gene Insert

    Depositor

    Bogumil Karas

    Article

    Cochrane et al Biodes Res. 2022 Aug 19;2022:9802168. do

    Insert

    HindII
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    ACT1 intron insert

    Promoter

    Available since

    May 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDA2ScIfm

    Plasmid

    #193786

    Purpose

    Expresses frame-shifted CI under the control of PLTATAA synthetic promoter, carries a synthetic fragment with additional restriction sites, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Frame-shifted CI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLTATAA synthetic promoter carrying binding sites…

    Available since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AP70_pU.CAG.Cas9-D10A-K848A-R1060A.rBGpA

    Plasmid

    #199254

    Purpose

    Expression construct encoding the high specificity SpCas9-KARA-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    High specificity S. pyogenes Cas9-D10A-KARA nickase
    

    Use

    CRISPR

    Tags

    3XFLAG epitope, Nucleoplasmin NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A K848A R1060A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLA2ScIfmTcIfm

    Plasmid

    #193792

    Purpose

    Cassette 1: Expresses frame-shifted CI under PLLacO-1 promoter, Cassette 2: Expresses frame-shifted CI under PLTetO-1 promoter, ColE1 origin of replication, Ampicillin selection

    Depositor

    Sangram Bagh

    Article

    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11

    Insert

    Two copies of frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PLLacO-1 and PLTetO-1

    Available since

    April 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAc5.1B-lambdaN-HA-DmNot1_1147-1717-K1277A_R

    Plasmid

    #147431

    Purpose

    Insect Expression of DmNot1_1147-1717-K1277A

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot1_1147-1717-K1277A (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    2 non silent mutations compared to the sequence g…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAc5.1B-lambdaN-HA-DmNot1_1149-2505-dsRNAres_R

    Plasmid

    #147432

    Purpose

    Insect Expression of DmNot1_1149-2505-dsRNAres

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot1_1149-2505-dsRNAres (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    6 silent mutations compared to the sequence given…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pnEA-NpG-DmNOT1_1-1083_R

    Plasmid

    #147441

    Purpose

    Bacterial Expression of DmNot1_1-1083

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot1_1-1083 (Not1 Fly)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    4 silent mutations and two non silent N605S, K759…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAc5.1B-EGFP-DmNot1_1-226_R

    Plasmid

    #147416

    Purpose

    Insect Expression of DmNot1_1-226

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot1_1-226 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 silent mutation compared to the sequence given …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAc5.1B-EGFP-DmNot1_1-412_R

    Plasmid

    #147417

    Purpose

    Insect Expression of DmNot1_1-412

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot1_1-412 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    1 silent mutation compared to the sequence given …

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAc5.1B-EGFP-DmNot1_416-1148_R

    Plasmid

    #147418

    Purpose

    Insect Expression of DmNot1_416-1148

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot1_416-1148 (Not1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    3 silent and two non silent N605S, K759M mutation…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAc5.1B-EGFP-DmNot2_1-436_R

    Plasmid

    #147419

    Purpose

    Insect Expression of DmNot2_1-436

    Depositor

    Elisa Izaurralde

    Article

    Bawankar et al RNA Biol. 2013 Feb;10(2):228-44. doi: 10

    Insert

    DmNot2_1-436 (Rga Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    one silent mutation C267T compared to the ORF ext…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge