We narrowed to 2,439 results for: plenti

1. pLenti gRNA KO OCLNx2 spCas9-T2A-iRFP670-P2A-puro

   Plasmid

   #208399

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, iRFP670 fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   iRFP670

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti gRNA KO OCLNx2 spCas9-T2A-Crimson-P2A-puro

   Plasmid

   #208398

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, E2-Crimson fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   E2-Crimson

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti gRNA KO OCLNx2 spCas9-T2A-mNeonGreen-P2A-puro

   Plasmid

   #208400

   Purpose

   codes for 2 gRNA-TracrRNA targeting human OCLN, Cas9, mNeonGreen fluorescent protein and puromycin resistance

   Depositor

   Raphael Gaudin

   Article

   Baptiste Pradel et al bioRxiv

   Insert

   hU6-gRNA and hH1-gRNA targeting OCLN gene (OCLN Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC

   Plasmid

   #202717

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and soluble bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC
   

   Use

   Lentiviral

   Tags

   10xHis and myc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-SMCHD1-Exon1-11E.12D-14.47M-48-3xFLAG

   Plasmid

   #130684

   Purpose

   Expresses human SMCHD1 Exon1-11E.12D-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-11E.12D-14.47M-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D453_F489del, E653_G1960del

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLenti-CMV-SMCHD1-Exon1-10A.11D-14.47M-48-3xFLAG

   Plasmid

   #130683

   Purpose

   Expresses human SMCHD1 Exon1-10A.11D-14.47M-48 with a C-terminal 3xFLAG tag

   Depositor

   Stephen Tapscott

   Article

   Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

   Insert

   SMCHD1 Exon1-10A.11D-14.47M-48 (SMCHD1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   D409_E452del, E653_G1960del

   Promoter

   CMV

   Available since

   Sept. 3, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #138149

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

   Insert

   CasRx
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

   Plasmid

   #207953

   Purpose

   nuclease inactive control for TRF1FokI WT

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI (D450A)

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNACRISPR_008-TetO-NLS-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #223173

   Purpose

   Expresses hfCas13d (N2V8) in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus.

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hfCas13d (N2V8)
   

   Use

   Lentiviral

   Tags

   NLS-HA

   Expression

   Mutation

   Promoter

   Available since

   Jan. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLentiRNACRISPR_009-TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-EGFP

    Plasmid

    #223174

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    Promoter

    Available since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

    Plasmid

    #202719

    Purpose

    Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

    Depositor

    Adam Cohen

    Article

    Xiang et al bioRxiv 2023

    Inserts

    Myr-Pink Flamindo
    EGFP-bPAC-CAAX
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

    Plasmid

    #122959

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xmyc tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

    Plasmid

    #122956

    Purpose

    Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta C terminus (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Expression

    Mutation

    deletion of C terminus from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE

    Plasmid

    #233047

    Purpose

    To express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' direction

    Depositor

    Jonathan Ploski

    Insert

    GFP and GluN2B
    

    Use

    Lentiviral

    Tags

    Flag and GFP

    Expression

    Mutation

    Promoter

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

    Plasmid

    #177130

    Purpose

    Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

    Depositor

    Wenbo Li

    Article

    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

    Insert

    YTHDC1 (YTHDC1 Human)
    

    Use

    Lentiviral

    Tags

    mClover3

    Expression

    Mutation

    W428A/W377A (m6A binding defects), and siRNA resi…

    Promoter

    Ef1a

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

    Plasmid

    #223175

    Purpose

    For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

    Depositor

    Neville Sanjana

    Article

    Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

    Insert

    hU6_Rfxcas13d-DR1_BsmBI
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    DR1 A>T

    Promoter

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid

    #163643

    Purpose

    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

    Depositor

    Chang Liu

    Article

    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

    Inserts

    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    

    Use

    Lentiviral

    Tags

    Luc2-P2A-tdTomato-T2A

    Expression

    Mutation

    The SpCas9 sgRNA constant region is mutated to ma…

    Promoter

    EFS and pU6

    Available since

    Feb. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228555

    Purpose

    Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    RfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA and P2A-mCherry

    Expression

    Mutation

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228557

    Purpose

    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dRfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228558

    Purpose

    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dhfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Expression

    Mutation

    R239A/H244A/R858A/H863A

    Promoter

    Available since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge