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Plasmid#227936PurposeComplementary plasmid providing gIIIDepositorInsertgIII (recoded)
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-HIS10-ZBASIC-TEV-GG
Plasmid#215797PurposeCloning of target gene with N-terminal His10 Zbasic tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pNIC- AVITAG-CTH10STII- -GG
Plasmid#215805PurposeCloning of target gene with C terminal non cleavable AVITAG and TEV cleavage of C terminal His10 and Twin Strep tagDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDSG546
Plasmid#214212PurposeArabinose-inducible folA (used in cross-feeding experiment)DepositorInsertAraC, folA
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG589
Plasmid#214217Purposecompetition integrase RBS library parent plasmid (myc-tagged)DepositorInsertPhlF, A118
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG553
Plasmid#214214Purposeprecut biphasic target plasmid version 1.0DepositorInsertAraC, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG588
Plasmid#214216Purposebiphasic target plasmid version 2.0 ("flip-proof")DepositorInsertfolA, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG545
Plasmid#214211Purposebiphasic target plasmid version 1.0DepositorInsertAraC, folA, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDSG550
Plasmid#214213PurposeArabinose-inducible trpC (used in cross-feeding experiment)DepositorInsertAraC, trpC
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCYP22
Plasmid#212855PurposeExpresses the Scytonema sp. HK-05 LRD-modifying cytochrome P450 enzymeDepositorInsertcytochrome P450
UseTagsExpressionBacterialMutationPromoterPcymRCAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLRD25
Plasmid#208272PurposeProduction of cis-abienol in E. coli MEV20DepositorInsertstHMGR
abCAS
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKP1411
Plasmid#167638PurposeExpress FepA I14C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA I14C
UseTagsExpressionBacterialMutationfepA with I14C mutation (Mutation is I36C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1383
Plasmid#167639PurposeExpress FepA V15C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V15C
UseTagsExpressionBacterialMutationfepA with V15C mutation (Mutation is V37C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1384
Plasmid#167641PurposeExpress FepA V16C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA V16C
UseTagsExpressionBacterialMutationfepA with V16C mutation (Mutation is V38C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1416
Plasmid#167643PurposeExpress FepA T17C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA T17C
UseTagsExpressionBacterialMutationfepA with T17C mutation (Mutation is T39C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1583
Plasmid#167645PurposeExpress FepA D34C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA D34C
UseTagsExpressionBacterialMutationfepA with D34C mutation (Mutation is D56C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP1382
Plasmid#167647PurposeExpress FepA G54C . Please note that mutation numbers are not based on start codon of insert.DepositorInsertfepA G54C
UseTagsExpressionBacterialMutationfepA with G54C mutation (Mutation is G76C based o…PromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2164
Plasmid#167608PurposeExpress ExbD V45A, L49ADepositorInsertexbD V45A, L49A
UseTagsExpressionBacterialMutationexbD with V45A, L49A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2171
Plasmid#167609PurposeExpress ExbD V45A, V47ADepositorInsertexbD V45A, V47A
UseTagsExpressionBacterialMutationexbD with V45A, V47A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP2182
Plasmid#167610PurposeExpress ExbD V45A,P50ADepositorInsertexbD V45A, P50A
UseTagsExpressionBacterialMutationexbD with V45A, P50A mutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only