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1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109314

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

Depositor

William Lagor

Publication

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pUC19-T7-3WJdB-T

Plasmid

#87308

Purpose

Expresses the fluorescence-activating Three-way Junction dimeric Broccoli (3WJdB) aptamer under the control of the T7 promoter.

Depositor

Donald Burke

Publication

Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

Insert

Three-way junction dimeric Broccoli aptamer

Use

Synthetic Biology

Expression

Bacterial

Promoter

T7

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMyc-N1

Plasmid

#85759

Purpose

A mammalian expression vector with a Myc-tag at C-terminus.

Depositor

Lei Lu

Publication

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCURE

Plasmid

#23013

Depositor

James Bowie

Publication

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

I-CreI homing endonuclease

Expression

Bacterial

Mutation

I-CreI expression is controlled by the arabinose …

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pSCT-GAL93-NONO

Plasmid

#46325

Depositor

Steven Brown

Publication

Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

Insert

NONO (NONO Human)

Expression

Mammalian

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only
D28 Cortactin GFP

Plasmid

#26723

Depositor

Anna Huttenlocher

Publication

Perrin et al Mol Biol Cell. 2006 Jan . 17(1):239-50.

Insert

Cortactin (Cttn Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion removed 28 amino acids (aa# 334-361) enc…

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pET_RGG

Plasmid

#124929

Purpose

Expression of RGG domain from LAF-1 gene

Depositor

Matthew Good, Daniel Hammer, Benjamin Schuster

Publication

Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

Insert

LAF-1 RGG domain (laf-1 Nematode)

Tags

His

Expression

Bacterial

Mutation

RGG domain

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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rASAP

Plasmid

#178205

Purpose

Ratiometric version of the genetically encoded voltage indicator ASAP2s-I67T-Q397R.

Depositor

Stefan Heinemann

Publication

Ruhl et al Commun Biol. 2021 Oct 7;4(1):1164. doi:

Insert

rASAP

Expression

Mammalian

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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KIF2C

Plasmid

#25551

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

KIF2C (KIF2C Human)

Tags

His

Expression

Bacterial

Available since

Aug. 25, 2010

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
pcDNA3-AktAR

Plasmid

#61624

Purpose

FRET-based biosensor for monitoring Akt activity

Depositor

Jin Zhang

Publication

Gao et al Mol Biol Cell. 2008 Oct;19(10):4366-73.

Insert

AktAR (AKT1 Human, Budding Yeast, Synthetic)

Tags

Cerulean and cpVenus[E172]

Expression

Mammalian

Promoter

CMV

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pET21-His6dNLP

Plasmid

#105498

Purpose

Expression of N-terminally 6His tagged Drosophila nucleoplasmin-like protein

Depositor

James Kadonaga

Publication

Khuong et al J Biol Chem. 2017 Nov 24;292(47):19478-1

Insert

His6dNLP

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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mNeon-Vamp2

Plasmid

#186619

Purpose

Encodes Vamp 2 fused to mNeonGreen

Depositor

Gia Voeltz

Publication

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Vesicle Associated Membrane Protein 2 (VAMP2 Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP Tubulin K40A

Plasmid

#105304

Purpose

To study effects of acetylation-null tubulin

Depositor

Kenneth Yamada

Publication

Joo et al Nat Commun. 2014 Mar 25;5:3510. doi: 10.

Insert

alpha tubulin (TUBA1B Human)

Tags

EGFP

Expression

Mammalian

Mutation

K40A

Available since

Feb. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pDD121

Plasmid

#91833

Purpose

Cas9 expression in C. elegans

Depositor

Bob Goldstein

Publication

Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

Insert

Cas9

Use

CRISPR

Expression

Worm

Mutation

Codon optimized and with synthetic introns for C.…

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTK81_GFP-4.1R

Plasmid

#46352

Depositor

Iain Cheeseman

Publication

Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

Insert

4.1R (EPB41 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Promoter

pBABE

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pJJiaDEST-APEX2-GAL8

Plasmid

#149519

Purpose

Express APEX2-Galectin-8 in mammalian cells

Depositor

Vojo Deretic

Publication

Jia et al Mol Cell. 2018 Apr 5;70(1):120-135.e8. d

Insert

LGALS8 (LGALS8 Human)

Tags

APEX2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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opto-Rac1

Plasmid

#135396

Purpose

Expresses opto-Rac1 (BcLOV4-GGGSx2-WT_Rac1-GGGSx2-mCherry in mammalian cells in the pcDNA3.1 backbone.

Depositor

Brian Chow

Publication

Berlew et al Photochem Photobiol Sci. 2020 Mar 1;19(3

Insert

opto-Rac1

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Dll1ICD-HPC4 pBacPAK8-3

Plasmid

#17333

Depositor

Joan Conaway, Ronald Conaway

Publication

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Delta-like 1 (Dll1 Mouse)

Tags

HPC4

Expression

Insect

Available since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pICH50892

Plasmid

#48046

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

2 Articles

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pclbw-opa1(isoform 7DeltaS1)-myc

Plasmid

#62846

Purpose

Mammalian Expression of myc-tagged Opa1 isoform 7 Delta S1

Depositor

David Chan

Publication

Mishra et al Cell Metab. 2014 Apr 1;19(4):630-41. doi

Insert

mmOPA1isoform7 DeltaS1 - myc (Opa1 Mouse)

Use

Retroviral

Tags

9xMyc

Expression

Mammalian

Promoter

cmv-bactin

Available since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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