Addgene

We narrowed to 9,116 results for: pho

Showing: 1541 - 1560 of 9116 results

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pLJM60 mUlk1 (wt) barcoded

Plasmid

#48799

Purpose

expresses wt Ulk1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

ULK1 (Ulk1 Mouse)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pRSV-p65T305A

Plasmid

#106454

Purpose

NF-kB p65, kinase site 305 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 305 mutated (RELA Human)

Use

Tags

Expression

Mammalian

Mutation

kinase site inactivated

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pNAS1B split mCherry N-link/link-C

Plasmid

#61965

Purpose

Negative control duet plasmid for split mCherry assembly assay (i.e., neither half of mCherry is fused to another peptide or protein)

Depositor

Lynne Regan

Article

Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.

Inserts

N-mCherry-link
link-C-mCherry

Use

Tags

Expression

Bacterial

Mutation

Promoter

PBAD and PLtetO

Available since

Feb. 13, 2015

Availability

Academic Institutions and Nonprofits only

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PIP2-CFP(ST) sensor

Plasmid

#170355

Purpose

CFP(ST)-PH fusion used to measure realtime changes in PIP2 levels.

Depositor

Kees Jalink

Article

van der Wal et al J Biol Chem. 2001 May 4. 276(18):15337-4

Insert

CFP(ST)-PH (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2021

Availability

Academic Institutions and Nonprofits only

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hRIP1 HA GFP K45A

Plasmid

#41389

Depositor

Francis Chan

Article

Cho et al Cell. 2009 Jun 12;137(6):1112-23.

Insert

RIP1 (RIPK1 Human)

Use

Tags

GFP and HA

Expression

Mammalian

Mutation

Kinase Domain Mutant (K45A)

Promoter

CMV

Available since

Dec. 3, 2012

Availability

Academic Institutions and Nonprofits only
pCMV-3xFlag-ETO

Plasmid

#12507

Depositor

Dong-Er Zhang

Article

Biggs et al Mol Cell Biol. 2006 Oct . 26(20):7420-9.

Insert

ETO (RUNX1T1 Human)

Use

Tags

3xFlag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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HA.PKD.K/W

Plasmid

#10809

Depositor

Alex Toker

Article

Storz et al J Biol Chem. 2003 May 16. 278(20):17969-

Insert

PKD (PRKD1 Human)

Use

Tags

HA and Myc (inserted between amino acids 33&3…

Expression

Mammalian

Mutation

Kinase dead mutant: K612W (Lys 612 to Trp) (bp183…

Promoter

CMV

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only
pCMV-myc-hIFITM3

Plasmid

#58461

Purpose

Expresses human IFITM3 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pMADM-beta

Plasmid

#36891

Depositor

Liqun Luo

Article

Tasic et al PLoS One. 2012;7(3):e33332. Epub 2012 Ma

Inserts

Beta-Geo
thymidine kinase

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG (chicken beta actin promoter and CMV enhancer…

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLJM60-S6K1(wt) barcoded

Plasmid

#48801

Purpose

expresses wt S6K1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

S6K1 (Rps6kb1 Rat)

Use

Lentiviral

Tags

FLAG and barcode: GGATCC

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only
pFastBac1-Flag-Timeless

Plasmid

#23119

Depositor

Aziz Sancar

Article

Kemp et al J Biol Chem. 2010 Mar 15. ():.

Insert

Timeless (TIMELESS Human)

Use

Tags

Flag

Expression

Insect

Mutation

Promoter

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL

Plasmid

#23090

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

Bim EL (Bcl2l11 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA-ALFA-tag-Gal4-VP16

Plasmid

#199465

Purpose

Expression of the Gal4-ALFA-tag-VP16 synthetic transcription factor containing the ALFA-tag sequence in mammalian cells.

Depositor

Vladislav Verkhusha

Article

Chernov et al Int J Mol Sci. 2023 Mar 30;24(7):6526. d

Insert

Gal4-ALFA-tag-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1-N100

Plasmid

#19848

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Aug 11. ():.

Insert

MKL1-N100 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mutation

deleted amino acids 1-100

Promoter

Available since

Jan. 13, 2009

Availability

Academic Institutions and Nonprofits only
Histone H2B targeted Aurora B FRET sensor Kif2 substrate

Plasmid

#45216

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

Histone H2B-targeted Aurora B FRET sensor

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 30, 2013

Availability

Academic Institutions and Nonprofits only

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pSIN REP5-GFP-GluR2 (Q)

Plasmid

#24003

Depositor

Robert Malinow

Article

Kessels et al Nat Neurosci. 2009 Jul . 12(7):888-96.

Insert

GluR2 (Gria2 Rat)

Use

Sindbis virus production

Tags

GFP

Expression

Mutation

R607Q

Promoter

Available since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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Caspase-6

Plasmid

#100102

Purpose

Bacterial expression of Caspase-6

Depositor

Jim Wells

Article

Seaman et al Cell Death Differ. 2016 Oct;23(10):1717-

Insert

Caspase-6 (CASP6 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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untargeted Plk1 FRET sensor Myt1 substrate

Plasmid

#45217

Depositor

Michael Lampson

Article

Fuller et al Nature. 2008 Jun 19;453(7198):1132-6. do

Insert

untargeted Plk1 FRET sensor Myt1 substrate

Use

Tags

Expression

Mammalian

Mutation

Promoter

t7

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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HIR Y1158,1162,1163F

Plasmid

#24054

Depositor

Frederick Stanley

Article

Jacob et al Mol Cell Endocrinol. 2002 Jan 15. 186(1)

Insert

Insulin Receptor (INSR Human)

Use

Tags

Expression

Mammalian

Mutation

Y1158F, Y1162F, Y1163F

Promoter

Available since

Dec. 16, 2010

Availability

Academic Institutions and Nonprofits only
pSMART-GFPuv

Plasmid

#65822

Purpose

GFPuv Expression, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

gfpuv

Use

Tags

Expression

Bacterial

Mutation

Promoter

E . coli insulated waaH gene promoter, low phosp…

Available since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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