Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,493 results for: D10

Clear keywords
Showing: 1561 - 1580 of 2493 results

  2. SpRY-Tad-CBE(ACC2)

    Plasmid
    #235081
    Purpose
    Expresses SpRY-Tad-CBE(ACC2) in mammalian cells.
    Depositor
    Weixin Tang
    Publication
    Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Tad-CBE(ACC2)-SpRY Cas9 D10A
    Use
    CRISPR
    Tags
    2xUGI, bpNLS and bpNLS
    Expression
    Mammalian
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    235081-plasmid-map-sequence-id-466818

    Enlarge

  4. pCRISPR-cBEST-v2-PermE

    Plasmid
    #209446
    Purpose
    improved pCRISPR-cBEST plasmid with optimized sgRNA cloning site
    Depositor
    Tilmann Weber
    Publication
    Maleckis et al ACS Chem Biol. 2024 Feb 10. doi: 10.1021
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Codon optimized APOBEC1-nCas9-UGI fusion protein
    Use
    CRISPR and Synthetic Biology
    Promoter
    PtipA; sgRNA expression from ermE* promoter
    Available since
    March 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    209446-plasmid-map-sequence-id-416632

    Enlarge

  6. pEditA

    Plasmid
    #207531
    Purpose
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA; SmR/SpR
    Depositor
    Pablo Ivan Nikel
    Publication
    Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Plasmid for adenine base editing; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ ABE:SpnCas9, PEM7→non-specific sgRNA
    Use
    CRISPR; Pseudomonas vector
    Available since
    Dec. 12, 2023
    Availability
    Academic Institutions and Nonprofits only
    207531-plasmid-map-sequence-id-409606

    Enlarge

  7. pET24a-ncOGT-TPR

    Plasmid
    #190822
    Purpose
    For expression of the TPR domain of human ncOGT (C467X mutation) in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.
    Depositor
    Gerald Hart
    Publication
    Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.
    Insert
    O-GlcNAc Transferase (OGT Human)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    C467X. Also codon optimized for expression in E.c…
    Promoter
    T7
    Available since
    Jan. 9, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    190822-plasmid-map-sequence-id-379182

    Enlarge

  8. JEH127

    Plasmid
    #179299
    Purpose
    Expresses dCas9 fused to VPR
    Depositor
    Keith Joung
    Publication
    Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-VPR
    Expression
    Mammalian
    Mutation
    D10A, H840A (catalytically inactive Cas9)
    Promoter
    CAG
    Available since
    Feb. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    179299-plasmid-map-sequence-id-354392

    Enlarge

  15. pLV-EF1A-CD19.8H.28TM.BB.28.3z

    Plasmid
    #200677
    Purpose
    Modular CD8hinge-CD28transmembrane-41BB-CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production
    Depositor
    Michele Bernasconi
    Publication
    Timpanaro et al J Exp Clin Cancer Res. 2023 Nov 4;42(1):
    Insert
    FMC63-CD8HD-CD28TM-41BB-CD28-CD3z-P2A-EGFP (CD8A, CD28, CD247, TNFRSF9 Human)
    Use
    Lentiviral
    Tags
    MycTag
    Expression
    Mammalian
    Promoter
    EF1a-short
    Available since
    Nov. 6, 2023
    Availability
    Academic Institutions and Nonprofits only
    200677-plasmid-map-sequence-id-391894

    Enlarge

  16. pLY70

    Plasmid
    #130948
    Purpose
    A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
    Depositor
    Baojun Wang
    Publication
    Liu et al Nat Commun. 2019 Aug 26;10(1):3693. doi:
    Inserts
    dxcas9 (3.7)
    tetR
    rhaS
    pspFΔHTH::λN22plus
    sfgfp
    Use
    Synthetic Biology
    Tags
    ASV tag and pspFΔHTH::λN22plus
    Expression
    Bacterial
    Mutation
    Mutations D10A and H840A on WT cas9 for dCas9; Sy…
    Promoter
    Pcon, PpspA-LEA3B3, PrhaB, and Ptet
    Available since
    Sept. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    130948-plasmid-map-sequence-id-257361

    Enlarge

  17. pCDNA5/FRT/TO_TOM20-dHaloTag7_T2A_Lifeact-HaloTag7

    Plasmid
    #187076
    Purpose
    Co-expression of dHaloTag7 at the mitochondria surface and HaloTag7 on actin filaments
    Depositor
    Kai Johnsson
    Publication
    Kompa et al J Am Chem Soc. 2023 Feb 8;145(5):3075-30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOM20-dHaloTag7_T2A_Lifeact-HaloTag7
    Expression
    Mammalian
    Mutation
    dHaloTag7 = HaloTag7-D106A
    Available since
    Aug. 5, 2025
    Availability
    Academic Institutions and Nonprofits only
    187076-plasmid-map-sequence-id-376955

    Enlarge

  18. pYPQAT37

    Plasmid
    #223409
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for dicot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by AtUBQ10 and the sgRNA was driven by AtU3; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-ecTadA8e-SpRY-D10A-AtU3-gRNA scaffold
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223409-plasmid-map-sequence-id-453514

    Enlarge

  19. pYPQAT42

    Plasmid
    #223414
    Purpose
    T-DNA vector for SpRY-D10A based A-to-G base editing for monocot plants; NA or NG PAM preference; SpRYD10A-ABE was driven by ZmUbi1 and the sgRNA was driven by ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Publication
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-ecTadA8e-SpRY-D10A-ZmUbi-gRNA scaffold-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223414-plasmid-map-sequence-id-454172

    Enlarge

Showing: 1561 - 1580 of 2493 results