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pAAV.Ple155-CI-EGFP-W3SL_BbsI(GGA)

Plasmid

#231363

Purpose

Ple155-driven EGFP, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

EGFP

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt1 (96-421) I367C

Plasmid

#213620

Purpose

Bacterial expression of SUMO-tagged rat syt1 (96-421) I367C

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt1 (96-421) I367C (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

C277A; I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 (136-403) L361C

Plasmid

#213622

Purpose

Bacterial expression of SUMO-tagged mouse syt7 (136-403) L361C

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 (136-403) L361C (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

C260A C275A L361C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-syt7 C2A-2A 2B

Plasmid

#213626

Purpose

Bacterial expression of SUMO-tagged mouse syt7 C2A-2A 2B

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

syt7 C2A-2A 2B (Syt7 Mouse)

Tags

SUMO

Expression

Bacterial

Mutation

F167A, F229A, R363H

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-FL-syt1 5W

Plasmid

#213629

Purpose

Bacterial expression of SUMO-tagged rat FL-syt1 5W

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

FL-syt1 5W (Syt1 Rat)

Tags

SUMO

Expression

Bacterial

Mutation

M173W, F231W, F234W, C277A, V304W, Y364W, I367C

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET SUMO-cPLA2 C2 domain (Y96C)

Plasmid

#213633

Purpose

Bacterial expression of SUMO-tagged human cPLA2 C2 domain (Y96C)

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

Insert

cPLA2 C2 domain (Y96C) (PLA2G4A Human)

Tags

SUMO

Expression

Bacterial

Mutation

Y96C, C139A, C141S

Available Since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-ChroMD-mRuby2-ST

Plasmid

#171158

Purpose

Cation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

Insert

ChroMD

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK3_IRS

Plasmid

#109885

Purpose

Protein expression and purification of DOK3_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK3_IRS (DOK3 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAB_14-3-3

Plasmid

#109904

Purpose

Protein expression and purification of YWHAB_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAB_14-3-3 (YWHAB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_TCEB1_POZ

Plasmid

#109875

Purpose

Protein expression and purification of TCEB1_POZ

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

TCEB1_POZ (ELOC Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOK2_IRS

Plasmid

#109884

Purpose

Protein expression and purification of DOK2_IRS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DOK2_IRS (DOK2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_HOMER3_WH1

Plasmid

#109891

Purpose

Protein expression and purification of HOMER3_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

HOMER3_WH1 (HOMER3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PREX2_DEP

Plasmid

#109893

Purpose

Protein expression and purification of PREX2_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PREX2_DEP (PREX2 Human)

Expression

Bacterial

Mutation

amino acids 369-474

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PREX2_DEP

Plasmid

#109894

Purpose

Protein expression and purification of PREX2_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PREX2_DEP (PREX2 Human)

Expression

Bacterial

Mutation

amino acids 472-576

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARF3_Arf

Plasmid

#109856

Purpose

Protein expression and purification of ARF3_Arf

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ARF3_Arf (ARF3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GGA3_VHS

Plasmid

#109824

Purpose

Protein expression and purification of GGA3_VHS

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GGA3_VHS (GGA3 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_CTDSP2_NIF

Plasmid

#109831

Purpose

Protein expression and purification of CTDSP2_NIF

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

CTDSP2_NIF (CTDSP2 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_GIGYF1_GYF

Plasmid

#109833

Purpose

Protein expression and purification of GIGYF1_GYF

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

GIGYF1_GYF (GIGYF1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_DEPDC1B_DEP

Plasmid

#109814

Purpose

Protein expression and purification of DEPDC1B_DEP

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

DEPDC1B_DEP (DEPDC1B Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_MYL6_EF-hand

Plasmid

#109794

Purpose

Protein expression and purification of MYL6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

MYL6_EF-hand (MYL6 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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