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We narrowed to 2,492 results for: PUC19

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Showing: 1561 - 1580 of 2492 results

  5. pINER4R

    Plasmid
    #185884
    Purpose
    In vivo gene amplification (HapAmp) of nerolidol synthetic module locus (25 copy).
    Depositor
    Claudia Vickers
    Article
    Peng et al Nat Commun. 2022 May 24;13(1):2895. doi:
    Insert
    PRPL25(Arm1)>Kl.LEU2>TKl.LEU2-PGAL1>ERG20>TRPL3-TRPL25(Arm3)-ARS305-PGAL2>Y.FAST-EVBR1.2A-AcNES1>TRPL41B-PBTS1>RPL25(part;Arm2)
    Expression
    Yeast
    Mutation
    None
    Available Since
    Sept. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    185884-plasmid-map-sequence-id-370832

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  8. pShHELIX(Cas12k-TniQ)-sgRNA_entry (CJT111)

    Plasmid
    #181790
    Purpose
    Expresses 3-component ShHELIX containing a Cas12k-TniQ fusion. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).
    Depositor
    Benjamin Kleinstiver
    Article
    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038
    Insert
    nAniI-ShTnsB, ShTnsC, ShCas12k-ShTniQ
    Expression
    Bacterial
    Mutation
    nAniI = K227M, F80K, L232K
    Promoter
    Lac and J23119
    Available Since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    181790-plasmid-map-sequence-id-354811

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  9. pNOC_hfnCas12a-Nlux_crRNA NR1

    Plasmid
    #176244
    Purpose
    pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the Nitrate reductase gene of N. oceanica IMET1 with spacer 1
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Promoter
    Ribosomal subunit
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176244-plasmid-map-sequence-id-346573

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  10. pNOC_dfnCas12a_crRNA NC

    Plasmid
    #176256
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    humanized fnCas12a
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    luciferase
    Mutation
    D917A and E1006A mutations to inactivate the endo…
    Available Since
    Oct. 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    176256-plasmid-map-sequence-id-346577

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  11. Gr4-T2A-QF2 HDR plasmid

    Plasmid
    #140944
    Purpose
    Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Gr4 gene
    Depositor
    Leslie Vosshall
    Article
    Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12
    Insert
    Gr4-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Gr4-right-HDR-arm
    Available Since
    Oct. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140944-plasmid-map-sequence-id-313602

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  12. pZNF423-donor

    Plasmid
    #113822
    Purpose
    CRISPR donor plasmid to tag human transcription factor ZNF423 with GFP
    Depositor
    Kevin White
    Insert
    ZNF423 homology arms flanking EGFP-IRES-Neo cassette (ZNF423 Human)
    Use
    CRISPR
    Available Since
    Dec. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    113822-plasmid-map-sequence-id-230193

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  13. pHOXB5-donor

    Plasmid
    #113815
    Purpose
    CRISPR donor plasmid to tag human transcription factor HOXB5 with GFP
    Depositor
    Kevin White
    Insert
    HOXB5 homology arms flanking EGFP-IRES-Neo cassette (HOXB5 Human)
    Use
    CRISPR
    Mutation
    rs2288276, rs7406798, rs36049616, rs3087996
    Available Since
    Dec. 4, 2018
    Availability
    Academic Institutions and Nonprofits only
    113815-plasmid-map-sequence-id-229913

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  15. AP1297‐1

    Plasmid
    #87247
    Purpose
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Depositor
    Geraldine Seydoux
    Article
    Paix et al Nucleic Acids Res. 2016 Sep 6;44(15):e12
    Insert
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::PH::gtbp‐ 1 (flanking sequence)
    Tags
    gtbp‐1 (flanking sequence)::eGFP::gtbp‐1 (27bp)::…
    Available Since
    Feb. 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    87247-plasmid-map-sequence-id-173228

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  20. pMT1035

    Plasmid
    #228300
    Purpose
    Constitutive expression of LuxTA mutant with strong promoter
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    luxTA mutant
    Use
    Synthetic Biology
    Tags
    Nuclear localization signal-Three copies of VP16 …
    Expression
    Yeast
    Mutation
    S116Y, W201R
    Promoter
    KpGAPDH promoter
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228300-plasmid-map-sequence-id-457086

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Showing: 1561 - 1580 of 2492 results