We narrowed to 2,364 results for: PUC19

1. pILFPQ13A7

   Plasmid

   #223837

   Purpose

   Expressing mScarletI3 under the control of the TEF1 promoter in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

   Insert

   pTEF1>mScarletI3>
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pILFPQ13B7

   Plasmid

   #223838

   Purpose

   Expressing StayGold under the control of the TEF1 promtoer in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

   Insert

   pTEF1>StayGold>
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDCX_mEmerald_CRISPR_HDR

   Plasmid

   #239331

   Purpose

   HDR template to insert mEmerald before the stop codon in exon 7 of the human doublecortin (DCX) locus

   Depositor

   Torsten Wittmann

   Article

   Dema et al Curr Biol. 2024 Nov 21:S0960-9822(24)014

   Insert

   mEmerald and DCX homology arms
   

   Use

   CRISPR

   Promoter

   None

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMDW108

   Plasmid

   #227194

   Purpose

   Template for multi-nucleosome array

   Depositor

   Michelle Wang

   Article

   Le et al Cell. 2019 Oct 17;179(3):619-631.e15. do

   Insert

   64mer array of p601 with 50bp flanking naked DNA between the anchor points and the first and the last 601.
   

   Expression

   Bacterial

   Promoter

   Plac

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pKE011

   Plasmid

   #207912

   Purpose

   Level 0 for gRNA in C4 module (5' GCAG-3' TCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS018

   Plasmid

   #207911

   Purpose

   Level 0 for gRNA in C3 module (5' TTCG-3' GCAG)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCS017

   Plasmid

   #207909

   Purpose

   Level 0 for gRNA in C1 module (5' GGCT-3' AGCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCS020

   Plasmid

   #207915

   Purpose

   Level 0 for gRNA in D3 module (5' TCCC-3' CTGC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU6-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKE012

   Plasmid

   #207914

   Purpose

   Level 0 for gRNA in AD2 module (5' TGAC-3' TCCC)

   Depositor

   Alexis Maizel

   Article

   Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

   Insert

   pU3-AarI-scaffold-term
   

   Use

   CRISPR

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKE010

    Plasmid

    #207910

    Purpose

    Level 0 for gRNA in C2 module (5' AGCC-3' TTCG)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU3-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCS019

    Plasmid

    #207913

    Purpose

    Level 0 for gRNA in D1 module (5' TCAG-3' TGAC)

    Depositor

    Alexis Maizel

    Article

    Piepers et al PLoS One. 2023 Sep 8;18(9):e0290097. doi

    Insert

    pU6-AarI-scaffold-term
    

    Use

    CRISPR

    Available Since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. NK621

    Plasmid

    #196405

    Purpose

    Strong expression of firefly luciferase codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. NK809

    Plasmid

    #196406

    Purpose

    Strong expression of firefly and nanoluc luciferases codon-optimized for S. rosetta

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5EFL-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. NK810

    Plasmid

    #196407

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-AaCas12b-T2A-EGFP

    Plasmid

    #188271

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    AaCas12b
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-PlmCasX-T2A-EGFP

    Plasmid

    #188273

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAcrVIB1-cloDF-amp

    Plasmid

    #184843

    Purpose

    Expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pILGFP4D42

    Plasmid

    #185840

    Purpose

    Testing the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pIALD2S

    Plasmid

    #185865

    Purpose

    Knocking out ALD6 and expressing anacetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP ) in Saccharomyces cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6 (-125, 40)> PADH1> Lr.pduP> TPDC1-ALD6 (1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILGFP10CF71

    Plasmid

    #185860

    Purpose

    Testing the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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