We narrowed to 4,484 results for: pET28

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pET28a-MBP-(CBX1)3-eReader

Plasmid

#135090

Purpose

Bacterial expression of engineered CBX1 fusions with enhanced binding to H3K9me

Depositor

Marcey Waters

Article

Albanese et al ACS Chem Biol. 2020 Jan 17;15(1):103-111

Insert

CBX1 chromobox protein 1, engineered x3

Tags

6xhis-tag and MBP

Expression

Bacterial

Mutation

lysine 43 mutated to alanine; aspartic acid 59 mu…

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pET28-His6-Ubc9-C138A

Plasmid

#133911

Purpose

Bacterial expression of His6-Ubc9-C138A

Depositor

Jeffrey Bode

Article

Hofmann et al Nat Chem. 2020 Nov;12(11):1008-1015. doi

Insert

SUMO-conjugating enzyme UBC9 (UBE2I Human)

Tags

Thrombin-cleavable His6-tag

Expression

Bacterial

Mutation

Cysteine 138 to Alanine

Promoter

T7

Available Since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-TAF4(827-909)

Plasmid

#105620

Purpose

bacterially express TAF4 HFD

Depositor

Christopher Vakoc

Article

Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

Insert

TAF4-HFD(827-909) (Taf4 Mouse)

Expression

Bacterial

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET28A-p53 (1-73) (P12A P13A)

Plasmid

#62066

Purpose

Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli

Depositor

Gary Daughdrill

Article

Borcherds et al Nat Chem Biol. 2014 Dec;10(12):1000-2. d

Insert

p53 (TP53 Human)

Tags

His

Expression

Bacterial

Mutation

Contains TAD residues 1-73; P12A, P13A

Promoter

T7

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only
pET28a-Hisx6-Sirt6-S56A

Plasmid

#20278

Depositor

Leonard Guarente

Article

Liszt et al J Biol Chem. 2005 Jun 3. 280(22):21313-2

Insert

Sirt6 (Sirt6 Mouse)

Tags

Hisx6

Expression

Bacterial

Mutation

Serine 56 to Alanine

Available Since

March 24, 2009

Availability

Academic Institutions and Nonprofits only
pET28b-NmCas9-Flag H588A

Plasmid

#109032

Purpose

H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expression

Depositor

Yan Zhang

Article

Rousseau et al Mol Cell. 2018 Mar 1;69(5):906-914.e4. d

Insert

CRISPR-associated endonuclease Cas9

Tags

Flag Tag

Expression

Bacterial

Promoter

T7

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-Bcx

Plasmid

#191195

Purpose

Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Bcx fusion

Tags

6xHis and Bacillus circulans xylanase

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-mScarlet-i

Plasmid

#191196

Purpose

Fusion of hfYFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-hfYFP-TEV-SAV

Plasmid

#191197

Purpose

Fusion of hfYFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

hfYFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-mScarlet-i

Plasmid

#191199

Purpose

Fusion of LSSmGFP and mScarlet-I separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & mScarlet-I fusion

Tags

6xHis and mScarlet-I

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LSSmGFP-TEV-SAV

Plasmid

#191200

Purpose

Fusion of LSSmGFP and Streptavidin core domain separated by a TEV cleavage site for fluorescence-assisted protein purification

Depositor

Gregory Petsko

Article

Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

Insert

LSSmGFP & Streptavidin fusion

Tags

6xHis and Streptavidin core domain, amino acids 1…

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-PKP3(305-797)

Plasmid

#202599

Purpose

Expresses ARM domain of plakophilin3

Depositor

Tina Izard

Article

Gupta et al Int J Mol Sci. 2023 May 29;24(11):9458.

Insert

plakophilin3 (PKP3 Human)

Tags

His8 tag with precision protease cleavage site

Expression

Bacterial

Mutation

Deleted amino acids 1 to 304

Available Since

Nov. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W2-W3-W4-HECT (381)

Plasmid

#187708

Purpose

Express his-tagged NEDD4L W2-W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 381-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-380

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-NEDD4L_W3-W4-HECT (492)

Plasmid

#187709

Purpose

Express his-tagged NEDD4L W3-W4-HECT in bacterial cells

Depositor

Sandra Gabelli

Article

Wright et al J Biol Chem. 2024 Mar;300(3):105715. doi

Insert

NEDD4L residues 492-975 (NEDD4L Human)

Tags

His-HRV 3C site

Expression

Bacterial

Mutation

deletion of residues 1-491

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-IRSp53(pLxIS)

Plasmid

#221336

Purpose

Overexpression of IRSp53 pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRSp53 pLxIS domain (Baiap2 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-MAVS(pLxIS)

Plasmid

#221335

Purpose

Overexpression of MAVS pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

MAVS pLxIS domain (Mavs Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-GMIP(pLxIS)

Plasmid

#221337

Purpose

Overexpression of GMIP pLxIS domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

GMIP pLxIS domain (Gmip Mouse)

Tags

6xHis-4xFKBP

Expression

Bacterial

Promoter

T7

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopTag-SpyTag-(AffiHER2)x3

Plasmid

#216281

Purpose

Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SnoopTag-SpyTag-(AffiHER2)x3

Use

Affinity Reagent/ Antibody

Tags

His6, SnoopTag, and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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