Addgene

We narrowed to 5,888 results for: pcDNA 3.1

Showing: 1561 - 1580 of 5888 results

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pcDNA3.1+_LN-HA-AGO2-5xE

Plasmid

#92011

Purpose

Expresses Lambda-N-HA-AGO2 [S824E, S828E, T830E, S831E, S834E (5xE)]

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Tags

Lambda-N-HA

Expression

Mammalian

Mutation

S824E, S828E, T830E, S831E, S834E (5xE)

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-long (pcDNA3.1+)

Plasmid

#75201

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon present

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/nV5-DEST-Apr1

Plasmid

#20135

Depositor

Valeri Vasioukhin

Article

Lien et al J Cell Biol. 2008 Dec 15. 183(6):989-97.

Insert

pcDNA3.1/nV5-DEST-Arp1 (Actr1a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-MC38-TCRA-30-picky_sticky

Plasmid

#138983

Purpose

IVT template for the alpha subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-33-MC_hammer

Plasmid

#138990

Purpose

IVT template for the beta subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-32-riot_punisher

Plasmid

#138988

Purpose

IVT template for the beta subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-32-riot_punisher

Plasmid

#138987

Purpose

IVT template for the alpha subunit of the mouse TCR #32 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-31-murderous_crow

Plasmid

#138986

Purpose

IVT template for the beta subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-31-murderous_crow

Plasmid

#138985

Purpose

IVT template for the alpha subunit of the mouse TCR #31 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRB-30-picky_sticky

Plasmid

#138984

Purpose

IVT template for the beta subunit of the mouse TCR #30 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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Human NCKX2-AS-short (pcDNA3.1+)

Plasmid

#75200

Purpose

Mammalian expression of SLC24A2

Depositor

Jonathan Lytton

Article

Visser et al J Biol Chem. 2007 Feb 16;282(7):4453-62.

Insert

SLC24A2 (SLC24A2 Human)

Use

Tags

Expression

Mammalian

Mutation

alternatively spliced exon absent

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 - HA-KLF4 FL delta K5S

Plasmid

#34594

Depositor

Michael Ruppert

Article

Lin et al Mol Cell Biol. 2011 Jun;31(12):2513-27.

Insert

KLF4 (KLF4 Human)

Use

Tags

HA tag

Expression

Mammalian

Mutation

deletion of K5S, corresponding to bases 1781 to 2…

Promoter

CMV

Available since

Jan. 24, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-myc-BirA*-NRASG12V

Plasmid

#122209

Purpose

Expresses myc-BirA* fused to NRASG12V in mammalian cells

Depositor

Christopher Counter

Article

Adhikari et al Nat Commun. 2018 Sep 7;9(1):3646. doi: 1

Insert

NRASG12v (NRAS Human)

Use

Tags

myc-BirA*

Expression

Mammalian

Mutation

changed G12 to V

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/TO-myc-BirA*-cCE

Plasmid

#112713

Purpose

For tetracycline-inducible expression of myc-BirA*-NES-mCE ΔNLS ("myc-BirA*-cCE") in mammalian cells. For BioID studies.

Depositor

Daniel Schoenberg

Article

Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

Insert

Rngtt (Rngtt Mouse)

Use

Tags

BirA* (BirA R118G), HIV Rev nuclear localization …

Expression

Mammalian

Mutation

Silent mutation at Arg 132 (CGT to CGC), deleted …

Promoter

CMV

Available since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _462-699

Plasmid

#86819

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1(-) + SLNCR1 _568-637

Plasmid

#86820

Purpose

mammalian expression of lncRNA LINC00673/SLNCR1 deletion construct

Depositor

Carl Novina

Article

Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

Insert

LINC00673 (LINC00673 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Char-BFP2-TSERex

Plasmid

#124792

Purpose

Expresses the proton pump rhodopsin in mammalian cells under CAG promoter

Depositor

Yulong Li

Article

Wu et al Elife. 2019 Jan 14;8. pii: 43366. doi: 1

Insert

Char-BFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 9, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-PGC-1alpha2

Plasmid

#45502

Depositor

Bruce Spiegelman, Jorge Ruas

Article

Ruas et al Cell. 2012 Dec 7;151(6):1319-31. doi: 10

Insert

PGC-1alpha Isoform2 (Ppargc1a Mouse)

Use

Tags

6xHis and Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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