1. Myr-(iSH2-p85)-p110alpha-Myc

   Plasmid

   #1410

   Depositor

   Lewis Cantley

   Insert

   PI3K catalytic subunit p110alpha-Myc- (iSH2-p85alpha) (Pik3ca, Pik3r1 Mouse)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   SRalpha promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

These results may also be relevant.

1. pBS-KS-attB2-SA(0)-T2A-3X Gal80-Hsp70

   Plasmid

   #62951

   Purpose

   Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 0. Gal80 is tagged with FLAG, HA and EGFP.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   3XGal80-FLAG/HA/EGFP-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Frt-V5-hspB5

   Plasmid

   #63106

   Purpose

   Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS-KS-attB2-SA(2)-T2A-3X Gal80-Hsp70

   Plasmid

   #62953

   Purpose

   Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   3XGal80-FLAG/HA/EGFP-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pBS-KS-attB2-SA(1)-T2A-3X Gal80-Hsp70

   Plasmid

   #62952

   Purpose

   Creating Gal80 lines from MiMIC lines with inserts into coding introns in Phase 1. Gal80 is tagged with FLAG, HA and EGFP.

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   3XGal80-FLAG/HA/EGFP-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS-KS-attB2-SA(0)-T2A-LexA::GADfluw-Hsp70

   Plasmid

   #78304

   Purpose

   Creating LexA::GADfluw lines from MiMIC lines with inserts into coding introns in Phase 0

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-nlsLexA::GADfluw-Hsp70
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Frt-hspB5

   Plasmid

   #63096

   Purpose

   Expression of HSPB5 (small HSP) in mammalian cells

   Depositor

   Harm Kampinga

   Article

   Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

   Insert

   hspb5 (CRYAB Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 6, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. cryS96

   Plasmid

   #67519

   Purpose

   αB-crystallin minipeptide fused to ELP (S96).

   Depositor

   Andrew MacKay

   Article

   Wang et al J Control Release. 2014 Oct 10;191:4-14.

   Insert

   mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
   

   Use

   Tags

   elastin-like polypeptide (ELP) S96

   Expression

   Bacterial

   Mutation

   mini-αB-crystallin contains residues 73–92 of aB-…

   Promoter

   T7

   Available since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

8. Mouse aB crystallin 1.5kb promoter/AcGFP

   Plasmid

   #98104

   Purpose

   Mouse aB crystallin 1.5kb promoter segment driving GFP expression

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha B-crystallin promoter 1.5 kb fragment, Mus musculus (Cryab Mouse)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Alpha B-crystallin 1.5 kb promoter fragment (-150…

   Available since

   Oct. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Mouse aB crystallin 250bp promoter/AcGFP

   Plasmid

   #98102

   Purpose

   Mouse aB crystallin 250bp promoter segment driving GFP expression

   Depositor

   Mason Posner

   Article

   Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

   Insert

   Alpha B-crystallin promoter 250 bp fragment, Mus musculus (Cryab Mouse)
   

   Use

   Gfp expressing

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Alpha B-crystallin promoter 250 bp fragment (-259…

   Available since

   Sept. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Mouse aB crystallin 800bp promoter/AcGFP

    Plasmid

    #98103

    Purpose

    Mouse aB crystallin 800bp promoter segment driving GFP expression

    Depositor

    Mason Posner

    Article

    Posner et al PeerJ. 2017 Nov 27;5:e4093. doi: 10.7717

    Insert

    Alpha B-crystallin promoter 800 bp fragment, Mus musculus (Cryab Mouse)
    

    Use

    Gfp expressing

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Alpha B-crystallin promoter 800 bp fragment (-833…

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET21a.MJ1228

    Plasmid

    #11410

    Depositor

    Sung-Hou Kim

    Article

    Kim et al Protein Sci. 1997 Oct . 6(10):2268-70.

    Insert

    eukaryotic translation initiation factor
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 10, 2006

    Availability

    Academic Institutions and Nonprofits only

12. pScaf-10080.1

    Plasmid

    #111410

    Purpose

    pScaf phagemid with insert for generating DNA origami scaffold 10080 bases in length

    Depositor

    Shawn Douglas

    Article

    Nafisi et al Synth Biol (Oxf). 2018 Jan;3(1). doi: 10

    Insert

    pScaf-ACKN
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pLenti-uORF1,2-MET

    Plasmid

    #214106

    Purpose

    MET 5'UTR upstream of mGFP (uORF1 and uORF2 mutated)

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

    Insert

    MET 5'UTR uORF1 and 2 mutated
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLenti_5'UTR_MET_GFP

    Plasmid

    #214103

    Purpose

    MET 5'UTR upstream of mGFP

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

    Insert

    MET 5'UTR
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Ube2d2

    Plasmid

    #31410

    Depositor

    Rachel Klevit

    Article

    Christensen et al Nat Struct Mol Biol. 2007 Oct . 14(10):9

    Insert

    Ubiquitin Conjugating Enzyme (UBE2D2 Human)
    

    Use

    Tags

    HA

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pLenti-uORF2-MET

    Plasmid

    #214105

    Purpose

    MET 5'UTR upstream of mGFP (uORF2 mutated)

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

    Insert

    MET 5'UTR uORF2 mutated
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-uORF1-MET

    Plasmid

    #214104

    Purpose

    MET 5'UTR upstream of mGFP (uORF1 mutated)

    Depositor

    Paolo Comoglio

    Article

    Cerqua et al EMBO J. 2025 Jan 7. doi: 10.1038/s44318-

    Insert

    MET 5'UTR
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    uORF1 mutation

    Promoter

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-EFIa-DIO-LMO3 (sbGLuc-VChR1-EYFP)

    Plasmid

    #114105

    Purpose

    Cre-dependent fusion protein of Gaussia luciferase variant slow burn, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Insert

    sbGLuc-VChR1-EYFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GST-TEVcs-FIP200-MBP

    Plasmid

    #171410

    Purpose

    For the purifcation of human ULK1 complex

    Depositor

    James Hurley

    Article

    Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

    Insert

    FIP200 (RB1CC1 Synthetic)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSEVA19_sgRNA-C0_M13ps

    Plasmid

    #231410

    Purpose

    M13 phagemid constitutively expressing sgRNA-C0. sgRNA-C0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI). (A gift of the Jaramillo Lab, where it is called PAJ156.)

    Depositor

    Alfonso Jaramillo

    Article

    Kushwaha et al Zenodo (2017) Conference: SB7.0: The Sev

    Insert

    sgRNA-C0
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge