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AiP14256 - pAAV-AiE0410h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN4256)

Plasmid

#230476

Purpose

AiE0410h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12561 - pAAV-AiE0359h_3xC1-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2561)

Plasmid

#230437

Purpose

AiE0359h_3xC1 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12568 - pAAV-AiE0226h_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2568)

Plasmid

#230363

Purpose

AiE0226h_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12560 - pAAV-AiE0373m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2560)

Plasmid

#230115

Purpose

AiE0373m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minBG

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12563 - pAAV-AiE0359h_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2563)

Plasmid

#229892

Purpose

AiE0359h_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific brain cell types

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12569 - pAAV-AiE0226h_3xC3-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2569)

Plasmid

#223995

Purpose

AiE0226h_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Sst+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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AiP12566 - pAAV-3xSP10ins-AiE0354m_3xC3-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2566)

Plasmid

#230438

Purpose

AiE0354m_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Promoter

minRho

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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AiP12565 - pAAV-3xSP10ins-AiE0354m_3xC2-minRho-SYFP2-WPRE3-BGHpA (Alias: CN2565)

Plasmid

#223994

Purpose

AiE0354m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in cortical Vip+ neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

Insert

SYFP2

Use

AAV

Mutation

NA

Promoter

minBG

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMLS256

Plasmid

#73715

Purpose

SapTrap destination vector for building combined sgRNA expression + repair template vectors

Depositor

Erik Jorgensen

Article

Schwartz et al Genetics. 2016 Feb 2. pii: genetics.115.

Insert

pU6::sgRNA

Use

CRISPR

Expression

Worm

Mutation

SapI insertion site

Promoter

C. elegans U6 snRNA pol III promoter

Available Since

March 25, 2016

Availability

Academic Institutions and Nonprofits only

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pJA256

Plasmid

#21509

Depositor

Julie Ahringer

Article

Zeiser et al PLoS One. 2011;6(5):e20082. Epub 2011 Ma

Insert

(Gly)5Ala::GFP(w introns)::tbb-2 3'UTR

Use

Multisite gateway

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only

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DCT.VV.Orf256

Plasmid

#12944

Purpose

This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

Depositor

Jack Bennink, Jon Yewdell

Article

Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

Insert

VV.Orf256

Expression

Mammalian

Available Since

Dec. 29, 2006

Availability

Academic Institutions and Nonprofits only
pGC256

Plasmid

#19656

Depositor

Jane Hubbard

Article

Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

Insert

Use

Flp/frt

Tags

4xNLS

Expression

Worm

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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KHBD00256

Plasmid

#34388

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG31666 (chinmo Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
TAL3256

Plasmid

#42719

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-foxl2-left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only
pJexpress414_OmpA171_H1256

Plasmid

#62648

Purpose

OmpA redesign hybrid with wild-type strands 1256

Depositor

Timothy Whitehead

Article

Stapleton et al Proc Natl Acad Sci U S A. 2015 Jul 21. p

Insert

OmpA_H1256

Use

Synthetic Biology

Tags

6His and FlAsH

Expression

Bacterial

Promoter

T7

Available Since

May 27, 2015

Availability

Academic Institutions and Nonprofits only
pCI-MOR256-17

Plasmid

#22369

Depositor

Hiroaki Matsunami

Article

Saito et al Sci Signal. 2009 . 2(60):ra9.

Insert

MOR256-17 (Olfr15 Mouse)

Tags

Rho

Expression

Mammalian

Available Since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pMOR256-17-ISH

Plasmid

#15528

Depositor

Peter Mombaerts

Article

Fuss et al Cell. 2007 Jul 27. 130(2):373-84.

Insert

MOR256-17 (Olfr15 Mouse)

Use

For riboprobe, in situ hybridization

Available Since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pRDA_256

Plasmid

#158581

Purpose

Lentiviral expression of BE3.9max and customizable gRNA

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Mutation

Cas9 D10A

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSLQ14256

Plasmid

#239275

Purpose

Expresses Kif5a*-PYL1 for the anterograde transport of RNA along microtubules via CRISPR-TO in primary mouse cortical neurons

Depositor

Stanley Qi

Article

Han et al Nature. 2025 Jul;643(8070):241-251. doi:

Insert

Kif5a(aa1-559)-BFP-PYL1 (Kif5a Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 10, 2025

Availability

Academic Institutions and Nonprofits only

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