Addgene

We narrowed to 21,801 results for: ESC;

Showing: 141 - 160 of 21801 results

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pESC-LEU2-RAD30cmyc

Plasmid

#60821

Purpose

to overexpress yeast polymerase eta in yeast

Depositor

Youri Pavlov, Thomas Kunkel

Article

Pavlov et al Mutat Res. 2001 Jul 1;478(1-2):129-39.

Insert

RAD30 (RAD30 Budding Yeast)

Use

Tags

c-myc

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only
pBluescript-KS(+) CREST (C90)

Plasmid

#22962

Depositor

Anirvan Ghosh

Insert

CREST (Ss18l1 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 29, 2010

Availability

Academic Institutions and Nonprofits only
HOXB5 (human) pBluescript

Plasmid

#8531

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB5 (HOXB5 Human)

Use

Tags

Expression

Bacterial

Mutation

makes protein in vitro transcription/translation

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
human EB1 in pBluescript II

Plasmid

#39324

Depositor

Tim Mitchison, Jennifer Tirnauer

Insert

EB1 (MAPRE1 Human)

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

T7

Available since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only
pBluescript-KS-Nrg3-ISH

Plasmid

#87669

Purpose

In situ probe for mouse Nrg3

Depositor

Oscar Marin

Article

Bartolini et al Cell Rep. 2017 Jan 31;18(5):1157-1170. d

Insert

Nrg3 (Nrg3 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7/M13

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_PM

Plasmid

#182813

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_cyto

Plasmid

#182814

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV(MinDis)-iEscSnFR_ER

Plasmid

#182812

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Article

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

Tags

Expression

Mammalian

Mutation

Promoter

hCMV

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pESC-LEU-IpgD

Plasmid

#183672

Purpose

Inducible Shigella flexneri IpgD for expression in yeast

Depositor

Sergio Grinstein

Article

Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

Insert

IpgD

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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564aa ELL2 bluescript

Plasmid

#127267

Purpose

For in vitro translation of human ELL2 lacking COOH region

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Tags

Expression

Mutation

Addgene Plasmid 127256--SphI site filled in, then…

Promoter

T7

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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ESCO2 C10.2 gRNA

Plasmid

#90681

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESCO2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESCO2 (Guide Designation C10.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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ESCO2 C9.2 gRNA

Plasmid

#90680

Purpose

3rd generation lentiviral gRNA plasmid targeting human ESCO2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ESCO2 (Guide Designation C9.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescript SK-rPAI-153

Plasmid

#45389

Depositor

Thomas Gelehrter

Article

Zeheb et al Gene. 1988 Dec 20;73(2):459-68.

Insert

Plasminogen activator inhibitor type 1 (Serpine1 Rat)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2013

Availability

Academic Institutions and Nonprofits only
pBluescript-IC3-FLAG-Tev-ZZ

Plasmid

#170311

Purpose

Plasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.

Depositor

Andrew Carter

Article

Mali et al Science. 2021 Feb 26;371(6532):910-916.

Insert

Outer Dynein Arm Intermediate Chain 3

Use

Tags

FLAG, ZZ

Expression

Mutation

Promoter

Available since

July 23, 2021

Availability

Academic Institutions and Nonprofits only

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HA GST PreScission p70 S6K1

Plasmid

#15511

Depositor

David Sabatini

Article

Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

Insert

p70 S6K1 (Rps6kb1 Rat)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2007

Availability

Academic Institutions and Nonprofits only
His6-TEV-mCerulean3-pBluescript

Plasmid

#166757

Purpose

For purification of mCerulean3 fluorophore

Depositor

David Andrews

Article

Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi

Insert

mCerulean3

Use

Tags

6x Histidine tag, and TEV protease site

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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pEF-pIgH-ESCargo(FTV)

Plasmid

#140160

Purpose

Expresses a regulatable secretory cargo for mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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