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Viral Prep#218871-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-GFAP-iGABASnFR2-WPRE (#218871). In addition to the viral particles, you will also receive purified pGP-AAV-GFAP-iGABASnFR2-WPRE plasmid DNA. GFAP-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsNoneAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only
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pGP-AAV-GFAP-iGABASnFR2-WPRE (AAV5)
Viral Prep#218871-AAV5PurposeReady-to-use AAV5 particles produced from pGP-AAV-GFAP-iGABASnFR2-WPRE (#218871). In addition to the viral particles, you will also receive purified pGP-AAV-GFAP-iGABASnFR2-WPRE plasmid DNA. GFAP-driven expression of the improved GABA sensor iGABASnFR2 (positive change in fluorescence). These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsNoneAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (AAV5)
Viral Prep#50472-AAV5PurposeReady-to-use AAV5 particles produced from pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine (#50472). In addition to the viral particles, you will also receive purified pAAV-GFAP-HA-rM3D(Gs)-IRES-mCitrine plasmid DNA. GFAP-driven HA-tagged rM3D(Gs) and bicistronic mCitrine expression, for chemogenetic excitation. These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsmCitrineAvailable sinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE (AAV1)
Viral Prep#218873-AAV1PurposeReady-to-use AAV1 particles produced from pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE (#218873). In addition to the viral particles, you will also receive purified pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE plasmid DNA. GFAP-driven expression of the improved GABA sensor control iGABASnFR2(no bind) (no change in fluorescence). These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsNoneAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE (AAV5)
Viral Prep#218873-AAV5PurposeReady-to-use AAV5 particles produced from pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE (#218873). In addition to the viral particles, you will also receive purified pGP-AAV-GFAP-iGABASnFR2(no bind)-WPRE plasmid DNA. GFAP-driven expression of the improved GABA sensor control iGABASnFR2(no bind) (no change in fluorescence). These AAV preparations are suitable purity for injection into animals.DepositorPromoterGFAPTagsNoneAvailable sinceSept. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSYC-204
Plasmid#178964PurposeHuman GFAP (R79C) mutant fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationR79CPromoterZebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-201
Plasmid#178963PurposeHuman GFAP fused to 3xFLAG tag and EGFP driven by zebrafish gfap promoter for Tol2 mediated recombinationDepositorInsertsUseTol2 mediated recombinationTags3xFLAGExpressionMutationPromoterzebrafish gfap promoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-nLac
Plasmid#53126PurposeExpression of LacZ driven by the human Gfa2 promoter. LacZ can also be replaced with the gene of interest for targeting transgene expression to astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman GFAPAvailable sinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSYC-176
Plasmid#178961PurposeExpresses human GFAP fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSYC-190
Plasmid#178962PurposeExpresses human GFAP (R79C) mutant fused to 3xFLAG tag in mammalian cellsDepositorInsertGlial fibrillary acidic protein (GFAP Human)
UseTags3xFLAGExpressionMammalianMutationR79CPromoterCMV IE94 PromoterAvailable sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGfaABC1D-nLac
Plasmid#53131Purposeexpression of lacZ driven by human GfaABC1D promoterDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains ABC1D regions of Gfa2 promoter, -1757 to…Promoterhuman Gfa2Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa28-nLac
Plasmid#53130PurposeExpression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.DepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutationcontains the A and B regions (bp -1757 to -1489) …Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-EGFP
Plasmid#53128PurposeExpression of EGFP in mouse astrocytesDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsEGFP and mP1ExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
ER-GCaMP6f-AAV
Plasmid#182501PurposeGFAP-ER-GCaMP6f AAV: An AAV5 constructed for ER-GCaMP6f with astrocyte specific GFAP promotorDepositorInsertER localized P450
UseAAVTagsExpressionMutationPromoterAvailable sinceApril 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C2-SNAP
Plasmid#208794PurposeFor AAV production to express C2-SNAP in astrocytesDepositorInsertC2-SNAP (Mfge8 Mouse)
UseAAVTagsSNAP-tagExpressionMammalianMutationPromoterGFAP promoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C2m2-mKate
Plasmid#208793PurposeFor AAV production to express C2m2-mKate in astrocytesDepositorInsertC2m2-mKate (Mfge8 Mouse)
UseAAVTagsmKateExpressionMammalianMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterGFAP promoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-C2m2-SNAP
Plasmid#208791PurposeFor AAV production to express C2m2-SNAP in astrocytesDepositorInsertC2m2-SNAP (Mfge8 Mouse)
UseAAVTagsSNAP-tagExpressionMammalianMutationMutated 24 and 45 lysine to asparagine in C2 doma…PromoterGFAP promoterAvailable sinceNov. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGfa2(ABDx3)-nLac
Plasmid#53133Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional copies of the gfa28 ABD segment; has about 100 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of 3 copies of the gf…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa(B3)-nLac
Plasmid#53132Purposeexpression of nuclear LacZ driven by Gfa2 promoter with 3 additional B sites, has about 75 times higher activity than pGfa2-nLac in transfected cellsDepositorInsertGfa2 (GFAP Human)
UseMouse TargetingTagsLacZ, NLS, and mP1ExpressionMammalianMutation-2163 to +47, with 3 copies of the gfa2 B segment…Promoterhuman Gfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGfa2-luc3
Plasmid#53129Purposeluciferase reporter for human gfa2 promoterDepositorInsertgfa2 promoter fragment (GFAP Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationcontains -2163 to +47 (the human gfa2 segment), w…Promotergfa2Available sinceJuly 30, 2014AvailabilityAcademic Institutions and Nonprofits only