We narrowed to 24,787 results for: Spr

1. pCRISPR2_IHFdist_rm

   Plasmid

   #162318

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   IHF distal site is removed

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR2_IRdist_scram

   Plasmid

   #162319

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   The distal Upstream Motif site is scrambled

   Promoter

   pBAD

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Shim_AspRS_TP_GFP_pK7FWG2

   Plasmid

   #202647

   Purpose

   Determine where transit peptide targets GFP in N. benthamiana

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from putative cytosolic AspRS
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Aug. 10, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MDM4_CRISPRi_gRNA_2

   Plasmid

   #195133

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 2 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MDM4_CRISPRi_gRNA_1

   Plasmid

   #195132

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 1 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR3_BC2

   Plasmid

   #191857

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR3_BC6

   Plasmid

   #191861

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR3_BC5

   Plasmid

   #191860

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR3_BC4

   Plasmid

   #191859

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR3_BC3

    Plasmid

    #191858

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCRISPR3_BC1

    Plasmid

    #191856

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CRISPR_HLA_DPB

    Plasmid

    #164989

    Purpose

    Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPB1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR_HLA_DQB

    Plasmid

    #164991

    Purpose

    Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQB1*05:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRISPR_HLA_DRB

    Plasmid

    #164992

    Purpose

    Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DRB1*01:02:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CRISPR_HLA_DQA

    Plasmid

    #164990

    Purpose

    Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQA1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRISPR_HLA_DPA

    Plasmid

    #164988

    Purpose

    Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPA1*02:01:08
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR2_wt

    Plasmid

    #149386

    Purpose

    P. aeruginosa PA14 CRISPR2 locus

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR2_-6D

    Plasmid

    #149387

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    6bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCRISPR2_-5D

    Plasmid

    #149388

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    5bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCRISPR2_+5D

    Plasmid

    #149390

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    5bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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