We narrowed to 24,827 results for: Spr;

1. pCRISPR2_IRdist_scram

   Plasmid

   #162319

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with a scrambled distal Inverted Repeat of the upstream motif

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   The distal Upstream Motif site is scrambled

   Promoter

   pBAD

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCRISPR2_IHFdist_rm

   Plasmid

   #162318

   Purpose

   P. aeruginosa PA14 CRISPR2 locus, with the IHFdistal binding site removed

   Depositor

   Blake Wiedenheft

   Article

   Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

   Insert

   Pseudomonas aeruginosa PA14 CRISPR2 locus
   

   Expression

   Bacterial

   Mutation

   IHF distal site is removed

   Available Since

   Dec. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Shim_AspRS_TP_GFP_pK7FWG2

   Plasmid

   #202647

   Purpose

   Determine where transit peptide targets GFP in N. benthamiana

   Depositor

   Daniel Sloan

   Article

   DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

   Insert

   N-terminal transit peptide from putative cytosolic AspRS
   

   Tags

   Green Florescent Protien

   Expression

   Plant

   Available Since

   Aug. 10, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. MDM4_CRISPRi_gRNA_2

   Plasmid

   #195133

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 2 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. MDM4_CRISPRi_gRNA_1

   Plasmid

   #195132

   Purpose

   gRNA from the Weissman CRISPRi library targeting MDM4, cloned into a third generation gRNA backbone with mCherry

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   MDM4 CRISPRi gRNA 1 (MDM4 Human)
   

   Expression

   Mammalian

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPR3_BC2

   Plasmid

   #191857

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: ATCATG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Jan. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCRISPR3_BC6

   Plasmid

   #191861

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CCTAGT) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCRISPR3_BC5

   Plasmid

   #191860

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: AGTCTA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCRISPR3_BC4

   Plasmid

   #191859

   Purpose

   Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GTATGA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

   Depositor

   Kimberly Reynolds

   Article

   Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Dec. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCRISPR3_BC3

    Plasmid

    #191858

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: GCATGG) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRISPR3_BC1

    Plasmid

    #191856

    Purpose

    Barcoded plasmid with BsaI sites to clone sgRNA. The 6-nucleotide barcode (sequence: CTTTCA) near the sgRNA insert region enables demultiplexing replicates while sequencing the sgRNA region.

    Depositor

    Kimberly Reynolds

    Article

    Otto et al Cell Syst. 2024 Feb 21;15(2):134-148.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Available Since

    Dec. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. CRISPR_HLA_DPB

    Plasmid

    #164989

    Purpose

    Expression of gRNA targeting HLA-DPB locus, including DPB1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPB1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. CRISPR_HLA_DQB

    Plasmid

    #164991

    Purpose

    Expression of gRNA targeting HLA-DQB locus, including DQB1*05:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQB1*05:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. CRISPR_HLA_DRB

    Plasmid

    #164992

    Purpose

    Expression of gRNA targeting HLA-DRB locus, including DRB1*01:02:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DRB1*01:02:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. CRISPR_HLA_DQA

    Plasmid

    #164990

    Purpose

    Expression of gRNA targeting HLA-DQA locus, including DQA1*01:01:01

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DQA1*01:01:01
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. CRISPR_HLA_DPA

    Plasmid

    #164988

    Purpose

    Expression of gRNA targeting HLA-DPA locus, including DPA1*02:01:08

    Depositor

    Matthew Meyerson

    Article

    Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

    Insert

    gRNA against HLA-DPA1*02:01:08
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCRISPR2_wt

    Plasmid

    #149386

    Purpose

    P. aeruginosa PA14 CRISPR2 locus

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCRISPR2_-6D

    Plasmid

    #149387

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 6bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    6bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR2_-5D

    Plasmid

    #149388

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp deletion downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    5bp deleted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPR2_+5D

    Plasmid

    #149390

    Purpose

    P. aeruginosa PA14 CRISPR2 locus, with 5bp addition downstream of IHFprox site

    Depositor

    Blake Wiedenheft

    Article

    Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

    Insert

    Pseudomonas aeruginosa PA14 CRISPR2 locus
    

    Expression

    Bacterial

    Mutation

    5bp inserted downstream of the IHFprox site

    Promoter

    pBAD

    Available Since

    Aug. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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