We narrowed to 152,534 results for: des

1. EGFPC2-CB2 SH3+

   Plasmid

   #68821

   Purpose

   EGFP tagged Collybistin expression in mammalian cells

   Depositor

   Shiva Tyagarajan

   Article

   de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

   Insert

   Collybistin (Arhgef9 Rat)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   Aug. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJEP14-AAV-H1/TO-sgRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82702

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO Empty gRNA Expression Cassette
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. PB-Ef1a-SunTag-RPS7-PP7-WPRE

   Plasmid

   #198338

   Purpose

   PB plasmid expressing RPS7 mRNA tagged with SunTag repeats and PP7 stem loops

   Depositor

   Michael Ward

   Article

   De Pace et al Nat Neurosci. 2024 Apr 10. doi: 10.1038/

   Insert

   GCN4x24-RPS7-3'UTR-PP7 stem loops x24
   

   Use

   Piggybac

   Tags

   PP7 stem loops and SunTag

   Expression

   Mammalian

   Mutation

   Promoter

   Ef1a

   Available since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRepair-mScI-CTNNB1

   Plasmid

   #153431

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-i

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   mScarlet-i

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pRepair-SGFP2-CTNNB1

   Plasmid

   #153430

   Purpose

   Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
   

   Use

   CRISPR; Hdr donor template

   Tags

   SGFP2

   Expression

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX459-CTNNB1-ATG

   Plasmid

   #153429

   Purpose

   Expresses a gRNA that overlaps the startcodon of human CTNNB1

   Depositor

   Renee van Amerongen

   Article

   de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

   Insert

   CTNNB1 gRNA (CTNNB1 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA 3.1 AnxA6

   Plasmid

   #20407

   Depositor

   Carlos Enrich

   Article

   Vila de Muga et al Oncogene. 2009 Jan 22. 28(3):363-77.

   Insert

   AnxA6 (Anxa6 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2009

   Availability

   Academic Institutions and Nonprofits only

8. pJEP15-AAV-H1/TO-sgRNA(Tet2)-CMV-TetR-P2A-eGFP-KASH-pA

   Plasmid

   #82701

   Purpose

   AAV backbone with a minimal H1/TO promoter driving expression of a sgRNA that targets exon 3 of the mouse Tet2 locus. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.

   Depositor

   Jonathan Ploski

   Article

   de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do

   Insert

   H1/TO gRNA Expression Cassette Containing Tet2 gRNA
   

   Use

   AAV and CRISPR

   Tags

   CMV promoter driving expression of TetR-P2A-GFP-K…

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Flag‐mTim1 (Bl/6)

   Plasmid

   #49205

   Purpose

   expresses Flag tagged mouse Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (BL/6 allele) (Havcr1 Mouse)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pRepair-SYFP2-CTNNB1

    Plasmid

    #153432

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    SYFP2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pKCAG-H2BmCherry-OptoJNKi

    Plasmid

    #89741

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to chromatin with H2B, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Optogenetics

    Tags

    Histone2B and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pmCherry-3xNLS-OptoJNKi-dsm

    Plasmid

    #89743

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi dark-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pmCherry-3xNLS-OptoJNKi-lsm

    Plasmid

    #89742

    Purpose

    Expression of light-regulated JNK inhibitor, mCherry-tagged, localised to nucleus, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    NLS-OptoJNKi lit-state mutant
    

    Use

    Fluorescent protein

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAX1_Z11 gpN:SpyTag

    Plasmid

    #225196

    Purpose

    Used for adding C-terminal SpyTag to the Plesiomonas ZOR0011 P2 phage major capsid protein

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    gpN homology arms and SpyTag
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAX1_HS_lexA(Ind-)

    Plasmid

    #225199

    Purpose

    Used to create a non-inducible lexA by introducing a G85D mutation in E. coli HS

    Depositor

    Travis Wiles

    Article

    Ortiz de Ora et al Nat Microbiol. 2025 Apr 18. doi: 10.1038

    Insert

    lexA homology arms
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    lexA G85D

    Promoter

    Available since

    April 18, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA 3.1-NbON10-NanoLuc

    Plasmid

    #198691

    Purpose

    Expresses anti-SARS-Cov-2 Nucleocapsid nanobody ON10 fused to NanoLuc in HEK293 cell culture

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, IgK leader sequence, NanoLuc, and twin…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pINQ-ON10

    Plasmid

    #198689

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody ON10 (HA-tagged) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody ON10
    

    Use

    Tags

    6xHis tag, HA tag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pINQ-NbH4

    Plasmid

    #198690

    Purpose

    Periplasmic expression of anti-SARS-Cov-2 Nucleocapsid nanobody H4 (AviTag) in BL21(DE3)

    Depositor

    Gualberto González Sapienza

    Article

    Segovia-de Los Santos et al Front Immunol. 2023 Jul 11;14:1220477. d

    Insert

    anti-SARS-CoV-2 Nucleocapsid nanobody H4
    

    Use

    Tags

    6xHis tag, Avitag, OmpA signal peptide, and Ribos…

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEMS2075

    Plasmid

    #158098

    Purpose

    High Copy number plasmid containing smCBA insert.

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    smCBA
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    smCBA

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pEMS2156

    Plasmid

    #158099

    Purpose

    High Copy number plasmid containing SYN1 insert

    Depositor

    Elizabeth Simpson

    Article

    de Moura Gomes et al Sci Rep. 2024 Nov 15;14(1):28194. doi: 1

    Insert

    SYN1 (SYN1 Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    SYN1

    Available since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

21. pNMSB96

    Plasmid

    #199316

    Purpose

    MosSCI insertion of flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR at ttTi5605

    Depositor

    Michael Krieg

    Article

    Porta-de-la-Riva et al Nat Methods. 2023 Apr 6. doi: 10.1038/s4

    Insert

    flp-18p::lox2272::mTagBFP2::tbb-2 3'UTR::lox2272::ACR1::SL2::jRGECO1a::let-858 3'UTR
    

    Use

    Tags

    Expression

    Worm

    Mutation

    mTagBFP2 from pJJR81, C. elegans codon optimized …

    Promoter

    flp-18

    Available since

    Aug. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRepair-mTq2-CTNNB1

    Plasmid

    #153433

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mTurquoise2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    mTurquoise2

    Expression

    Mutation

    Promoter

    Available since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pLuc-Optop38i-lsm

    Plasmid

    #89747

    Purpose

    Expression of light-regulated p38 inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor MK3BD3-13F

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    Optop38i lit-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLuc-OptoJNKi-lsm

    Plasmid

    #89746

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, lit-state mutant photosensor (AsLov2Ja.I539E), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi lit-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    I539E mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLuc-OptoJNKi

    Plasmid

    #89744

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, wild-type photosensor, inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pLuc-OptoJNKi-dsm

    Plasmid

    #89745

    Purpose

    Expression of light-regulated JNK inhibitor, firefly luciferase-fused, dark-state mutant photosensor (AsLov2Ja.C450A), inhibitor JIP11

    Depositor

    Michael Courtney

    Article

    Melero-Fernandez de Mera et al Nat Commun. 2017 May 12;8:15017. doi: 10

    Insert

    OptoJNKi dark-state mutant
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Expression

    Mammalian

    Mutation

    C450A mutation of AsLov2Jalpha

    Promoter

    CMV

    Available since

    Aug. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEMS1241

    Plasmid

    #29123

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple12
    

    Use

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pEMS1207

    Plasmid

    #29104

    Purpose

    Expression of the eGFP reporter gene was not detected in the brain and eye.

    Depositor

    Elizabeth Simpson

    Article

    de Leeuw et al Mol Ther Methods Clin Dev. 2014 Jan 8;1:

    Insert

    Ple235
    

    Use

    Pleiades promoter project [sic, pleaides plieades]

    Tags

    EGFP-NLS

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pZE27GFP

    Plasmid

    #75452

    Purpose

    Plasmid for constitutive expression of gfp

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pB33eCPX

    Plasmid

    #23336

    Depositor

    Patrick Daugherty

    Article

    Rice et al Protein Eng Des Sel. 2008 Jul . 21(7):43

    Insert

    Enhanced Circularly Permuted Outer Membrane Protein X
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 9, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pZE27MC3

    Plasmid

    #75464

    Purpose

    Plasmid for constitutive expression of mcherry-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    mcherry-pdt#3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGRB2.3

    Plasmid

    #45343

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Tags

    Expression

    Mutation

    Promoter

    PGK1

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pECJ3

    Plasmid

    #75465

    Purpose

    Toggle switch plasmid containing lacI-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    lacI-pdt3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pZE27GFP3

    Plasmid

    #75456

    Purpose

    Plasmid for constitutive expression of gfp-pdt3

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp-pdt#3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pZE27GFP5

    Plasmid

    #75458

    Purpose

    Plasmid for constitutive expression of gfp-pdt5

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp-pdt#5
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pECJ3B

    Plasmid

    #75466

    Purpose

    Toggle switch plasmid containing lacI-pdt3B

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    lacI-pdt3B
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pGRB2.2

    Plasmid

    #45342

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Tags

    Expression

    Mutation

    Promoter

    PGK1

    Available since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pECJ3D

    Plasmid

    #75467

    Purpose

    Toggle switch plasmid containing lacI-pdt3D

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    lacI-pdt3D
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pLtetO

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pZE27GFP3E

    Plasmid

    #75463

    Purpose

    Plasmid for constitutive expression of gfp-pdt3E

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp-pdt#3E
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    placIq promoter

    Available since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCN-ACO2

    Plasmid

    #45329

    Depositor

    Brendan Cormack

    Article

    Zordan et al G3 (Bethesda). 2013 Oct 3;3(10):1675-86.

    Type

    Empty backbone

    Use

    C. glabrata expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge