We narrowed to 2,205 results for: D10

1. pDicAID_nCas9-PmCDA_NptII_Della

   Plasmid

   #91694

   Purpose

   Dicot Target-AID vector expressing dicot-optimized nCas9-PmCDA1 with sgRNA targeting SlDella

   Depositor

   Akihiko Kondo

   Article

   Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

   Insert

   SpCas9
   

   Use

   Tags

   PmCDA1

   Expression

   Plant

   Mutation

   D10A for nickase Cas9

   Promoter

   PcUbi

   Available since

   June 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA GNSTM-3-Flag-10-Lamp2b-HA

   Plasmid

   #71293

   Purpose

   Encodes (N to C): GNSTM glycosylation motif, 3 residue spacer, Flag tag,10 residue spacer, Lamp2b (exosomal transmembrane protein), 3 residue spacer, HA tag.

   Depositor

   Joshua Leonard

   Article

   Hung et al J Biol Chem. 2015 Mar 27;290(13):8166-72

   Insert

   Lamp2b (LAMP2 Human)
   

   Use

   Tags

   FLAG, GNSTM glycosylation motif, HA, and Lamp2 si…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. STIM1-EGFP

   Plasmid

   #210370

   Purpose

   Expresses STIM1 fused with EGFP in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   STIM1 (STIM1 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFP5380 LAMP1-SBP- mCherry-FIREtag

   Plasmid

   #216725

   Purpose

   Expresses LAMP1-mCherry-FIREtag in mamallian cells

   Depositor

   Franck Perez

   Article

   Bottone et al Nat Methods. 2023 Aug 28. doi: 10.1038/s

   Insert

   LAMP-1 (LAMP1 Human)
   

   Use

   Tags

   FIREtag, Streptavidin Binding Protein (SBP), and …

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. ABE8.20-m

   Plasmid

   #136300

   Purpose

   expresses ABE8.20-m in mammalian cells

   Depositor

   Nicole Gaudelli

   Article

   Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

   Insert

   ABE8.20-m
   

   Use

   Tags

   C-terminal BPNLS

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-Delta(Pro-MP)ADAM10-HA

   Plasmid

   #65107

   Purpose

   Expression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

   Insert

   Delta(Pro-MP)ADAM10 (ADAM10 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   deleted AA 19-455

   Promoter

   CMV

   Available since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pcDNA3-Lyso-Digda

   Plasmid

   #236102

   Purpose

   Lysosome-targeted FRET-based biosensor for monitoring diacylglycerol (DAG) dynamics in living cells.

   Depositor

   Jin Zhang

   Article

   Su et al Nat Chem Biol. 2024 Oct 11. doi: 10.1038

   Insert

   Lyso-Digda (LAMP1 Human, Synthetic)
   

   Use

   Tags

   Full-length LAMP1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. ICAM2-bio-His

   Plasmid

   #51758

   Purpose

   Expresses full-length Intercellular adhesion molecule 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   ICAM2 (ICAM2 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-sp-flag-hSema4D

   Plasmid

   #190656

   Purpose

   Expresses Human Sema4D with N terminal flag after signal peptide

   Depositor

   Victor Tarabykin, Andrew Newman

   Article

   Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

   Insert

   Sema4D (SEMA4D Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. AXBEv2

    Plasmid

    #204606

    Purpose

    Mammalian expression of evolved adenine transversion base editor AXBEv2

    Depositor

    Dali Li

    Article

    Chen et al Nat Biotechnol. 2023 Jun 15. doi: 10.103

    Insert

    bpNLS-TadA-8e-nSpCas9-mAAG (R165E/Y179F)-bpNLS-P2A-GFP
    

    Use

    CRISPR

    Tags

    6*His

    Expression

    Mammalian

    Mutation

    mAAG (R165E/Y179F); S. pyogenes Cas9 (D10A)

    Promoter

    Available since

    Aug. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. SpCas9 TadCBEd-V106W

    Plasmid

    #193839

    Purpose

    Express TadCBEd-V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

    Insert

    TadA-CDd V106W-SpCas9 D10A-2xUGI
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

    Plasmid

    #102886

    Purpose

    PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti PGK mKate2-OGT WT (Puro)

    Plasmid

    #154290

    Purpose

    Lentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Lentiviral

    Tags

    2x FLAG and mKate2

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    PGK

    Available since

    Dec. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. ABE8.8-m

    Plasmid

    #136294

    Purpose

    expresses ABE8.8-m in mammalian cells

    Depositor

    Nicole Gaudelli

    Article

    Gaudelli et al Nat Biotechnol. 2020 Apr 13. pii: 10.103

    Insert

    ABE8.8-m
    

    Use

    Tags

    C-terminal BPNLS

    Expression

    Mammalian

    Mutation

    D10A in S. pyogenes Cas9, TadA mutations describe…

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCRISPR-aBEST

    Plasmid

    #131464

    Purpose

    Expression of a Streptomyces codon optimized Cas9n-tadA fusion protein in pGM1190. A to G base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Inserts

    Streptomyces codon optimized spCas9n (D10A)
    streptomyces codon optimized tadA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    tipA

    Available since

    Nov. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-Target-ACEmax

    Plasmid

    #139105

    Purpose

    Mammalian Target-ACEmax expression vector (pSI-625)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-PmCDA1-UGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-hA3A-BE3

    Plasmid

    #113410

    Purpose

    Expresses hA3A-BE3 in mammalian cells

    Depositor

    Jia Chen

    Article

    Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

    Insert

    hA3A-BE3 (APOBEC3A Human, S. pyogenes and Bacteriophage PBS2)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Integrin β4-GFP

    Plasmid

    #205092

    Purpose

    Express GFP-tagged human integrin β4 in mammalian cells

    Depositor

    Bianxiao Cui

    Article

    Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038

    Insert

    Integrin beta 4 (ITGB4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-sp-myc-mSema4D

    Plasmid

    #190645

    Purpose

    Expresses Mouse Sema4D with N-terminal myc after endogenous Signal peptide

    Depositor

    Victor Tarabykin, Andrew Newman

    Article

    Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

    Insert

    Sema4d (Sema4d Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-ABERA-P2A-Puro

    Plasmid

    #112675

    Purpose

    Lentivirus for constitutive expression of ABE7.10 RA (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    ABERA
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    D10A

    Promoter

    EF1s

    Available since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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