We narrowed to 4,346 results for: SE

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pECIA14

Plasmid

#47051

Purpose

This is a Gateway destination vector for secreted expression in Drosophila cell culture by CuSO4 induction. Inserts will be pentamerized and tagged with Alkaline phosphatase, FLAG and His tags

Depositor

Chris Garcia

Article

Ozkan et al Cell. 2013 Jul 3;154(1):228-39. doi: 10.

Type

Empty backbone

Use

Secreted expression in drosophila culture

Tags

Alkaline Phosphatase (human, placental), Flag, HR…

Expression

Insect

Promoter

Metallothionein (Copper-Inducible)

Available Since

Aug. 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-EWB-DIO-cyan pre-eGRASP(p30)

Plasmid

#111583

Purpose

An AAV vector that expresses double floxed cyan pre-eGRASP(p30) under the Ef1a promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

cyan pre-eGRASP(p30)

Use

AAV

Promoter

Ef1a

Available Since

June 12, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Ac-AChBP

Plasmid

#63128

Purpose

Expression of Aplysia californica AChBP

Depositor

Titia Sixma

Article

Celie et al Nat Struct Mol Biol. 2005 Jul;12(7):582-

Insert

AChBP

Expression

Insect

Promoter

Polyhedrin

Available Since

March 13, 2015

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-IRES-Puro GFP-3xFLAG

Plasmid

#201636

Purpose

Over-expression of 3xFLAG-tagged GFP

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

GFP

Use

Lentiviral

Tags

3xFLAG

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pHH0103_YWHAE_14-3-3

Plasmid

#109942

Purpose

Protein expression and purification of YWHAE_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

YWHAE_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pSJ1568 (pRS315-NOP1pr-GFP11-mCherry-SCS2TM)

Plasmid

#86416

Purpose

ONM/ER split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP11-mCherry-Scs2TM

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_SFN_14-3-3

Plasmid

#109937

Purpose

Protein expression and purification of SFN_14-3-3

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

SFN_14-3-3

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSL3936 (pDonor(AAVS1)_Pse)

Plasmid

#200903

Purpose

Encodes a mini-transposon derived from Pseudoalteromonas Tn7016 with a SA-T2A-PuroR-T2A-eGFP cargo. Total transposon size = 2,024 bp.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

Mini Tn7016 (AAVS1 NGS)

Expression

Mammalian

Available Since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TIWB-myrTagRFP-T-P2A-post-eGRASP

Plasmid

#111590

Purpose

An AAV vector that expresses myristoylated TagRFP-T and post-eGRASP linked by self-cleaving P2A peptide under the tetO promoter.

Depositor

Bong-Kiun Kaang

Article

Choi et al Science. 2018 Apr 27;360(6387):430-435.

Insert

myrTagRFP-T-P2A-post-eGRASP

Use

AAV

Promoter

tetO

Available Since

June 7, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_PDCD6_EF-hand

Plasmid

#110009

Purpose

Protein expression and purification of PDCD6_EF-hand

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PDCD6_EF-hand (PDCD6 Human)

Expression

Bacterial

Available Since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pSJ1602 (pRS315-NOP1pr-mCherry-SCS2TM-GFP11)

Plasmid

#86417

Purpose

luminal split GFP11 reporter with mCherry

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-mCherry-SCS2TM-GFP11

Tags

GFP11 and mCherry

Expression

Yeast

Promoter

NOP1

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBSII-PGK-FLPo-IRES-Puro

Plasmid

#205989

Purpose

Expresses Flp recombinase and puromycin resistance

Depositor

Sharon Torigoe

Article

Waite et al PLoS One. 2024 Sep 30;19(9):e0311120. do

Inserts

PGK promoter
FLPo (flp )
IRES-PuroR cassette

Expression

Mammalian

Promoter

PGK promoter

Available Since

Oct. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pHH0103_UBA52_Ubiquitin

Plasmid

#110043

Purpose

Protein expression and purification of UBA52_Ubiquitin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

UBA52_Ubiquitin (UBA52 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_PFN1_Profilin

Plasmid

#110028

Purpose

Protein expression and purification of PFN1_Profilin

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PFN1_Profilin (PFN1 Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSJ2039 (pRS316-NOP1pr-GFP1-10-SCS2TM)

Plasmid

#86418

Purpose

ONM/ER split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

NOP1pr-GFP1-10-Scs2TM

Tags

GFP1-10

Expression

Yeast

Promoter

NOP1

Available Since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pHH0103_PRKACB_Pkinase

Plasmid

#110024

Purpose

Protein expression and purification of PRKACB_Pkinase

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

PRKACB_Pkinase (PRKACB Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pSL0375 (His-MBP-TniQ_Cas8_Cas7_Cas6)

Plasmid

#130640

Purpose

Expresses V. cholerae CAST TniQ, Cas8, Cas7, and Cas6 from a T7 promoter, with N-terminal His10-MBP-TEVsite tag on TniQ for purification.

Depositor

Samuel H. Sternberg

Article

Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

Insert

VchTniQ, VchCas8, VchCas7, VchCas6

Use

CRISPR; Transposon

Tags

His10-MBP-TEVsite on TniQ

Expression

Bacterial

Promoter

T7

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG_EGFP-GFE3

Plasmid

#79869

Purpose

Expresses under the CAG promoter GFE3, a synthetic E3 ligase that rapidly degrades endogenous Gephyrin at inhibitory synapses.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Promoter

Beta-actin

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCDNA-3xFLAG-NLS-TPP1

Plasmid

#53585

Purpose

Mammalian expression of 3xFLAG tagged, nuclear localized TPP1, N-terminal.

Depositor

Steven Artandi

Article

Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

Insert

TPP1 (ACD Human)

Tags

3xFLAG and NLS

Expression

Mammalian

Mutation

Silent mutation to eliminate PacI site

Promoter

CMV

Available Since

June 9, 2014

Availability

Academic Institutions and Nonprofits only