We narrowed to 11,554 results for: Vars;

1. B-HeFSpCas9-A1003K

   Plasmid

   #207371

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A1003K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A, amino …

   Promoter

   CBh

   Available Since

   Sept. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. B-HeFSpCas9-A848K

   Plasmid

   #207369

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A848K
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. B-HeFSpCas9-A661R

   Plasmid

   #207367

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9-A661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   N497A, Q695A, K848A, Q926A, K1003A, R1060A, amino…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. B-evoSpCas9-Q661R

   Plasmid

   #207366

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-Q661R
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, Y515N, K526E, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. B-evoSpCas9-N515Y

   Plasmid

   #207364

   Purpose

   Expression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variant

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-evoSpCas9-N515Y
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   M495V, K526E, R661Q, amino acids 1005-1013 replac…

   Promoter

   CBh

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. PET-B-HeFSpCas9-NLS-6xHis

   Plasmid

   #207392

   Purpose

   Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

   Insert

   B-HeFSpCas9
   

   Use

   CRISPR

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, K1003A, R1060A…

   Promoter

   T7

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOPINF Ta-sro1 PARP

   Plasmid

   #192549

   Purpose

   E. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)

   Depositor

   Lennart Wirthmueller

   Article

   Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

   Insert

   SIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
   

   Use

   Tags

   His6, 3C protease cleavage site

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   Promoter

   T7 promoter

   Available Since

   Dec. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. Donor_C_FH_VIR

   Plasmid

   #186660

   Purpose

   Donor plasmid to endogenously tag VIR gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS VIR C-terminal 3xFlag-3xHA Tag Donor Plasmid (vir Fly)
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. Donor_N_FH_Shu

   Plasmid

   #186655

   Purpose

   Donor plasmid to endogenously tag Shu gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   OSS Shu N-terminal 3xFlag-3xHA Tag Donor Plasmid (shu Fly)
   

   Use

   Tags

   3xFlag-3xHA

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pDonor_N_FH_GLE

    Plasmid

    #186657

    Purpose

    Donor plasmid to endogenously tag GLE gene in Drosophila melanogaster ovarian somatic sheet (OSS) cells.

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    OSS Gle N-terminal 3xFlag-3xHA Tag Donor Plasmid (CG14749 Fly)
    

    Use

    Tags

    3xFlag-3xHA

    Expression

    Insect

    Mutation

    Promoter

    Available Since

    July 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. S+Q111R

    Plasmid

    #167173

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with Q111R mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+Q111R
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed Q at 111 to R

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. S+N122D

    Plasmid

    #167174

    Purpose

    Expresses the full Leptodactylus latrans Na+K+ATPase with ATP1A1-S variant with N122D mutation in Sf9 cells

    Depositor

    Susanne Dobler

    Article

    Mohammadi et al Curr Biol. 2021 Jun 21;31(12):2530-2538.

    Inserts

    ATP1A1-S+N122D
    ATP1B1
    

    Use

    Tags

    Expression

    Bacterial and Insect

    Mutation

    Changed N at 111 to D

    Promoter

    P10 and PH

    Available Since

    Feb. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETM33_Nsp3c_SUD-C

    Plasmid

    #156471

    Purpose

    Bacterial expression of Sars-CoV2 Nsp3c_SUD-C protein with His-tag and GST-tag

    Depositor

    Ylva Ivarsson

    Article

    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

    Insert

    Nsp3c_SUD-C (ORF1ab Synthetic)
    

    Use

    Tags

    His-GST

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX-FHH-NRASQ61K/T35A-IRES-Puro

    Plasmid

    #120571

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K/T35A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K, T35A

    Promoter

    CMV

    Available Since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLEX-FHH-NRASQ61K/Y40A-IRES-Puro

    Plasmid

    #120573

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K/Y40A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K, Y40A

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLEX-FHH-NRASQ61K/E37A-IRES-Puro

    Plasmid

    #120572

    Purpose

    Expresses Flag-HA-6xHIS-N-Ras Q61K/E37A fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    NRAS (NRAS Human)
    

    Use

    Lentiviral

    Tags

    Flag-HA-6xHIS

    Expression

    Mammalian

    Mutation

    Q61K, E37A

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-mt-mKeima-P2A-FRB-Fis1

    Plasmid

    #135295

    Purpose

    stably express both mKeima and FRB-Fis1 for CID induced mitophagy in mammalian cells

    Depositor

    Richard Youle

    Article

    Vargas et al Mol Cell. 2019 Apr 18;74(2):347-362.e6.

    Insert

    mt-mKeima-P2A-FRB-Fis1 (FIS1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    mt-mKeima is fused with FRB-Fis1 via P2A seqeunce

    Promoter

    Available Since

    Jan. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-T7-SpG-P2A-EGFP (RTW4177)

    Plasmid

    #139988

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpG(D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpG with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

    Plasmid

    #120561

    Purpose

    Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

    Depositor

    Paul Khavari

    Article

    Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    KRAS4B (KRAS Human)
    

    Use

    Lentiviral

    Tags

    HA-birA*

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3.1-FGFR3c-V5/HIS

    Plasmid

    #201108

    Purpose

    expression of human FGFR3 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR3 receptor tyrosine kinase, variant c, full length, wildtype (FGFR3 Human)
    

    Use

    Tags

    V5/His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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