We narrowed to 3,357 results for: phage

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pET-CV-Ykt6

Plasmid

#168030

Purpose

Expresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Ykt6(Q131-K190)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBT138b-pro1

Plasmid

#138513

Purpose

P3h

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pBT138b-proA

Plasmid

#138514

Purpose

P3g

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPP378

Plasmid

#158797

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-1(D371A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-1 (D371A)

Expression

Bacterial

Available Since

Aug. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pJT42_GalL_BE-PAPAPA

Plasmid

#145035

Purpose

Expresses BE-PAPAPA in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pMR-A-4

Plasmid

#138518

Purpose

P3b

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pPP384

Plasmid

#158799

Purpose

pRSF-Duet1 derived plasmid containing C-terminally hexa-histidine tagged dCasΦ-3(D413A) in MCS1 for expression in E. coli and protein purification

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

dCasPhi-3 (D413A)

Expression

Bacterial

Available Since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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fADL-1e-flag-linker-p3

Plasmid

#139441

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage.

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, flag-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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fADL-1e-HA-linker-p3

Plasmid

#139440

Purpose

For cloning and subsequent expression of peptide fragments fused with pIII protein as a part of fd filamentous bacteriophage

Depositor

Alexander Gabibov

Article

Ishina et al Int J Mol Sci. 2020 Aug 29;21(17). pii:

Insert

linker with pelB-leader, cloning site, HA-tag and p3

Expression

Bacterial

Available Since

Aug. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pET-CV-VAMP2

Plasmid

#168023

Purpose

Expresses eCFP-VAMP2(T27-R86)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP2(T27-R86)-mVenus

Tags

Hisx6

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-λN-βLac

Plasmid

#162039

Purpose

Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)

Depositor

Karen Polizzi

Article

Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199

Insert

λN domain of the λ phage and a β-lactamase fusion protein

Tags

6xHis tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pJT23_GalL_BE-PAPAP

Plasmid

#143486

Purpose

Expresses BE-PAPAP in yeast cells

Depositor

Ralph Bock

Article

Tan et al Nat Commun. 2019 Jan 25;10(1):439. doi:

Insert

BE-PAPAP

Use

CRISPR

Expression

Yeast

Mutation

spCas9(D10A)

Promoter

GalL

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoPL

Plasmid

#114467

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPL

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx TOM20 mVenus BoLL

Plasmid

#114465

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoLL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoLL

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoLD

Plasmid

#114468

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoLD

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoLD

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTBY1-T4gp61/41 fusion

Plasmid

#228210

Purpose

Expression in BL21(DE3) of T4 primase and helicase fusion with 24 amino acid linker

Depositor

Stephen Benkovic

Article

Manosas et al Nat Chem Biol. 2009 Dec;5(12):904-12. do

Insert

61/41

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pOD_003

Plasmid

#155362

Purpose

Zeomycin version of the scarless genome engineering pDEL plasmid (Tikh et al. 2016) compatible with Salmonella

Depositor

Elisabeth Raleigh

Article

Zaworski et al PLoS Genet. 2022 Apr 4;18(4):e1009943. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Sept. 30, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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AP-VAMP1(X)

Plasmid

#167999

Purpose

positive selection PACE Accessory Plasmid

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

VAMP1(L56-L72)-T7PAP

Use

Synthetic Biology

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pJH191

Plasmid

#172149

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.2TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*, Y184*); [strong promoter eG, strong RB…

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pJH190

Plasmid

#172148

Purpose

Accessory Plasmid for PACE of pylBCD variants

Depositor

Matthew Bennett

Article

Ho et al Nat Commun. 2021 Jun 24;12(1):3914. doi:

Insert

Ppsp gIII.1TAG, luxAB; Plpp [eG SD8] 32A-Nter; PproK pylT

Expression

Bacterial

Mutation

gIII(P29*); [strong promoter eG, strong RBS SD8] …

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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