We narrowed to 1,719 results for: plasmid for e coli

1. pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)

   Plasmid

   #191643

   Purpose

   E. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementation

   Depositor

   Emily Balskus

   Article

   Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

   Insert

   DadR
   

   Use

   Tags

   3xFLAG

   Expression

   Bacterial

   Mutation

   Linker region sequence shuffled

   Promoter

   Available Since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

   Plasmid

   #182305

   Purpose

   E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.

   Depositor

   Andrew York

   Article

   Seidel et al (2021), doi: 10.5281/zenodo.5810930

   Insert

   pH-Countdown insertion into GECO 1.2 calcium sensing domains
   

   Use

   Tags

   A modified TorA periplasmic targeting sequence pl…

   Expression

   Bacterial

   Mutation

   Spontaneous mutation of calmodulin's penulti…

   Promoter

   t7

   Available Since

   April 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX6P1-optimizedNedd4.2F823D

   Plasmid

   #165105

   Purpose

   bacterial expression of a GST fusion of Human Nedd4.2d F823D

   Depositor

   Arthur Haas

   Article

   Todaro et al J Biol Chem. 2018 Nov 23;293(47):18192-1

   Insert

   Nedd4.2d F823D (NEDD4L Human)
   

   Use

   Tags

   GST from plasmid.

   Expression

   Bacterial

   Mutation

   codon optimized for E. coli expression; F823D; …

   Promoter

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJK174

   Plasmid

   #71835

   Purpose

   Expression of Acetobacter aceti 1023 PurE and PurK in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Inserts

   N5-carboxyaminoimidazole ribonucleotide mutase
   N5-carboxyaminoimidazole ribonucleotide synthetase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   additional N-terminal methionine encoded by this …

   Promoter

   T7 and endogenous

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJK175

   Plasmid

   #71132

   Purpose

   Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli

   Depositor

   T. J. Kappock

   Article

   Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20

   Insert

   N5-carboxyaminoimidazole ribonucleotide mutase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   additional N-terminal methionine encoded by this …

   Promoter

   T7

   Available Since

   Jan. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pMCherry F8-2

   Plasmid

   #186034

   Purpose

   Express VHH F8-2 as a mCherry fusion in mammalian cells

   Depositor

   Isabelle Landrieu

   Article

   Danis et al Mol Ther. 2022 Apr 6;30(4):1484-1499. do

   Insert

   VHH F8-2
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV immediate early

   Available Since

   July 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. p164-pAAV-mDlx-FLEX-mScarlet

   Plasmid

   #204689

   Purpose

   Fluorescent reporter under the control of the Dlx promotor

   Depositor

   Lynette Lim

   Article

   Fisher et al Neuron. 2023 Dec 7:S0896-6273(23)00887-5

   Insert

   mScarlet
   

   Use

   AAV and Cre/Lox

   Tags

   mScarlet palmitoylation

   Expression

   Mammalian

   Mutation

   Promoter

   mDlx

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV-syn-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234437

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-syn-iGluSnFR4f-NGR-WPRE

   Plasmid

   #234438

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Has Service

   AAV1

   Insert

   iGluSnFR4f-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV-syn-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234435

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234448

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-GFAP-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234445

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pAAV-GFAP-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234446

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-iGluSnFR4s-NGR-WPRE

    Plasmid

    #234451

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAAV-syn-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234436

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-CAG-iGluSnFR4f-NGR-WPRE

    Plasmid

    #234452

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-NGR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-NGR GPI anchor

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-CAG-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234447

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-syn-flex-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234440

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CAG-iGluSnFR4f-PDGFR-WPRE

    Plasmid

    #234450

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4f-PDGFR
    

    Use

    AAV

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-syn-flex-iGluSnFR4s-PDGFR-WPRE

    Plasmid

    #234439

    Purpose

    AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

    Depositor

    GENIE Project

    Article

    Aggarwal et al bioRxiv 2025.03.20.643984

    Insert

    iGluSnFR4s-PDGFR
    

    Use

    AAV and Cre/Lox

    Tags

    IgK chain and Myc epi tag-PDGFR TM Domain

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge