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pCas9-EcRT-GAL-Hyg

Plasmid

#232091

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-GAL-HIS3

Plasmid

#232093

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCas9-EcRT-GAL-Nat

Plasmid

#232090

Purpose

Yeast CEN plasmid for galactose-inducible expression of spCas9 and Ec86 reverse transcriptase.

Depositor

Jeffrey Lewis

Article

Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

Insert

SpCas9 and Ec86 reverse transcriptase

Use

CRISPR

Expression

Yeast

Mutation

Ec86 reverse transcriptase is codon optimized for…

Promoter

GAL1-GAL10

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pET28C-mCherry-SARS-CoV-2-Nucleocapsid

Plasmid

#204403

Purpose

E. coli expression of full-length SARS-CoV-2 nucleocapsid with an N-terminal mCherry tag

Depositor

Samie Jaffrey

Article

Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

Insert

SARS-CoV-2 nucleocapsid (N SARS-CoV-2)

Tags

6x-His; mCherry

Expression

Bacterial

Promoter

T7

Available Since

Sept. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CoV-2-NSP3-c001

Plasmid

#173083

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))

Tags

mCerulean

Expression

Bacterial

Mutation

E1084-E1192, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARF6-V5-His6

Plasmid

#67415

Purpose

Expression of hArf6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF6 (ARF6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
CoV-2-NSP3-c002

Plasmid

#173084

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Article

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

rep (ORF1ab Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2))

Expression

Bacterial

Mutation

E1084-E1192, codon optimized

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL8B-V5-His6

Plasmid

#67425

Purpose

Expression of hArl8b with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF1-V5-His6

Plasmid

#67411

Purpose

Expression of hArf1 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF1 (ARF1 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL6-V5-His6

Plasmid

#67422

Purpose

Expression of hArl6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF3-V5-His6

Plasmid

#67412

Purpose

Expression of hArf3 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF3 (ARF3 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF4-V5-His6

Plasmid

#67413

Purpose

Expression of hArf4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF4 (ARF4 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARF5-V5-His6

Plasmid

#67414

Purpose

Expression of hArf5 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARF5 (ARF5 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL2-V5-His6

Plasmid

#67417

Purpose

Expression of hArl2 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL2 (ARL2 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL8A-V5-His6

Plasmid

#67424

Purpose

Expression of hArl8 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8A (ARL8A Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL4D

Plasmid

#67384

Purpose

Expression of native hArl4D in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL11

Plasmid

#67386

Purpose

Expression of native hArl11 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Expression

Bacterial

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-29b(+)_NSP4ss_SerM_NucA-His

Plasmid

#218190

Purpose

Bacterial expression of a periplasmically expressed His-tagged Serratia Marcescens NucA (Benzonase)

Depositor

Florence Pojer

Article

Rieubon et al protocols.io 2026. doi: https://dx.doi.o

Insert

nucA

Tags

10xHis and NSP4 signal secretion peptide

Expression

Bacterial

Mutation

codon optimized for E. coli, residues 22-266

Promoter

T7

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET29_anti-MERS-Nanobody-VHH-84

Plasmid

#209419

Purpose

Express anti-MERS-Nanobody-VHH-84 in E. coli

Depositor

Magnus Rueping

Article

Peng et al ACS Synth Biol. 2025 Jan 9. doi: 10.1021

Insert

anti-mers-nanobody-vhh-83

Tags

6xHis

Expression

Bacterial

Available Since

Jan. 13, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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