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pMW96_6xHis-Sumo-TEV-mNG

Plasmid

#253320

Purpose

bacterial expression and purification of mNG

Depositor

Kristen Verhey

Article

Geng et al EMBO J. 2024 Aug;43(15):3192-3213. doi:

Insert

mNG

Tags

His-SUMO

Expression

Bacterial

Available Since

March 30, 2026

Availability

Academic Institutions and Nonprofits only

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EGFP-SEC24C

Plasmid

#250870

Purpose

Transient mammalian expression of EGFP-SEC24C

Depositor

Thomas Melia

Article

Fuller et al eLife 2025 14:RP107316

Insert

SEC24C (SEC24C Human)

Expression

Mammalian

Available Since

March 4, 2026

Availability

Academic Institutions and Nonprofits only

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pESUMO-RsmD-9AAYBBR

Plasmid

#250651

Purpose

Plasmid for expression of E.coli methyltransferase protein RsmD tagged after 9 amino acids with a YBBR tag

Depositor

Olivier Duss

Article

Gor et al Sci Adv. 2026 Mar 20;12(12):eaec4037. do

Insert

RsmD (rsmD )

Tags

YBBR

Expression

Bacterial

Mutation

YBBR tag inserted after the 9th Amino acid from t…

Promoter

T7

Available Since

Feb. 20, 2026

Availability

Academic Institutions and Nonprofits only

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pTL52_pyCAG-s36GFPHaloTag-IRES-mCherry3NLS-polyA

Plasmid

#223544

Purpose

Minimal PUFFHalo cassette with s36GFP-HaloTag and mCherry-3NLS, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-HaloTag, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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pTL53_pyCAG-s36GFPmNG-IRES-mCherry3NLS-polyA

Plasmid

#223545

Purpose

Minimal PUFFFIN cassette with s36GFP-mNeonGreen and mCherry-3NLS, separated by IRES, strong constitutive promoter CAG, no insulation or mammalian selection gene

Depositor

Sally Lowell

Article

Lebek et al EMBO J. 2024 Jul 12. doi: 10.1038/s44318

Insert

s36GFP-mNeonGreen, mCherry-3NLS

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 21, 2026

Availability

Academic Institutions and Nonprofits only

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FSW FLAG

Plasmid

#246725

Purpose

Neuron-specific expression of insert with a FLAG tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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FSW mKeima

Plasmid

#246726

Purpose

Neuron-specific expression of insert with a mKeima tag on its N-terminus after cloning

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2025 Aug 25. doi: 10.1038

Type

Empty backbone

Use

Lentiviral

Tags

mKeima

Expression

Mammalian

Available Since

Dec. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti_DualsgRNA_CTRL_ER-dAPEX-BFP2

Plasmid

#236733

Purpose

Dual sgRNA targeting CTRL expressing dAPEX-BFP targeting to ER

Depositor

Derek Narendra

Article

Thayer et al EMBO J. 2025 Nov 20. doi: 10.1038/s44318

Insert

Negative CTRL

Use

CRISPR and Lentiviral

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pX459-Fzd1/2 gRNA

Plasmid

#246562

Purpose

CRISPR vector co-expressing Cas9 and a mouse Fzd1/2 gRNA (conserved region)

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

Fzd1/2 gRNA

Use

CRISPR

Promoter

U6

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pBABE-Puro-BirA

Plasmid

#246557

Purpose

Retroviral expression vector for secreted E. coli BirA, C-terminal FLAG tag

Depositor

Mario Vallon

Article

Heiden et al J Biol Chem. 2025 Sep 4:110682. doi: 10.

Insert

BirA

Use

Retroviral

Tags

FLAG

Promoter

MMLV LTR

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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