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Plasmid#188600PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK216E [AAA=> GAA]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625 CM]
Plasmid#188588PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler C-terminal domain, catalytic mutant
UseTagsFLAG-MycExpressionInsectMutationD435A [GAT=>GCC]; E435A [GAA=>GCC]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Mm)
Plasmid#188589PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and GWpep mmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K276A]
Plasmid#188597PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK276A [AAG=>GCG]PromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[C399-625]-Gwpep(Dm)
Plasmid#188590PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) C-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc and Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Dm)-Nbr[C399-625]
Plasmid#188592PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (drosophila) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep dmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Gwpep(Mm)-Nbr[C399-625]
Plasmid#188591PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertGW-peptide (mouse) N-terminally tagged C-terminal domain of Nibbler
UseTagsFLAG-Myc-Gwpep mmExpressionInsectMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A,K276A]
Plasmid#188596PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG], K276A [AAG=>GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K284A]
Plasmid#188595PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK284A [AAG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[R272A]
Plasmid#188594PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationR272A [CGG=> GCG]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr[K187A]
Plasmid#188593PurposeFlag-tagged Nibbler variants expression in Drosophila S2 cellsDepositorInsertNibbler (Nbr Fly)
UseTagsFLAG-MycExpressionInsectMutationK187A [AAA=> GCA]PromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pA-RFP-rG2
Plasmid#188969PurposeIPTG inducible mCherry with sgRNADepositorInsertsmCherry
sgRNA: agtccatgtaatcagcgtctactagt
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPtrcAvailable sinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLiP-G2
Plasmid#188964PurposeL-Rhamnose inducible catalytically inactive Cas9 with sgRNA for bacterial gene knockdownDepositorInsertsdCas9
sgRNA: gttagacgctgattacatggactagg
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaBADAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G
Plasmid#146403PurposeBacterial Expression of DmDCP1_328-366-mut1DepositorInsertDmDCP1_328-366-mut1 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G
Plasmid#146404PurposeBacterial Expression of DmDCP1_328-366-mut2DepositorInsertDmDCP1_328-366-mut2 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G
Plasmid#146405PurposeBacterial Expression of DmDCP1_328-366-mut3DepositorInsertDmDCP1_328-366-mut3 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G
Plasmid#146406PurposeBacterial Expression of DmDCP1_328-366DepositorInsertDmDCP1_328-366 (Dcp1 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mSpata33-PA
Plasmid#179129PurposeExpression vector of mouse spermatogenesis associated 33 (Spata33) tagged with PA at C-terminus, CAG promoter, rabbit globin poly(A) signal.DepositorInsertspermatogenesis associated 33 (Spata33 Mouse)
UseTagsPAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-0-mTagBFP2(11x7)
Plasmid#172064PurposeProtein trap plasmid for inserting mTagBFP2(11x7) into a phase 0 coding intronDepositorInsertmTagBFP2(11x7)
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV-TREtight-H2b-emiRFP670-P2A-TEVP
Plasmid#174379PurposeAAV virus for expressing TEVP and H2b-emiRFP670DepositorInsertTEVP
UseAAVTagsExpressionMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRV∆G-N*PEST-4Cre
Plasmid#174381PurposeRabies vector for expressing Cre recombinase and N gene with a stop codon before PEST domainDepositorInsertCre recombinase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
Mdn1-deltaC
Plasmid#171603PurposeExpresses S. pombe Mdn1 a.a. 1-3911 in insect cells.DepositorInsertMdn1
UseTagsHis6 tagExpressionInsectMutationC-terminal deletion; a.a. 1-3911 onlyPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLIC-His-GBAi
Plasmid#171755PurposeFor the expression of His-GBAi in bacteriaDepositorInsertGBAi
UseTagsHisExpressionBacterialMutationPromoterAvailable sinceJune 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMRBad-Z-C-DiB-RM
Plasmid#168477PurposeC-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168475: pET11a-Z-N-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertLeucine Zipper + C-fragment of DiB-RM
UseTagsExpressionBacterialMutationPromoteraraBADAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET11a-Z-N-DiB-RM
Plasmid#168475PurposeN-fragment of the DiB-RM‐split‐Zip protein. This plasmid needs to be co-transformed with Plasmid 168477: pMRBad-Z-C-DiB-RM to obtain DiB-RM‐split-Zip proteinDepositorInsertN-fragment of DiB-RM + Leucine Zipper
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14A)-mVenus
Plasmid#168499PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9R)-mVenus
Plasmid#168498PurposeMammalian expression of the K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9R mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN(K8,9A)-mPAGFP
Plasmid#168494PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.DepositorInsertK8,9A mutant of the N-terminal 47 residues of mouse PKA Catalytic subunit α C-terminally tagged by monomeric paGFP.
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (E12,14K)-mVenus
Plasmid#168488PurposeMammalian expression of the E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14K mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9E)-mVenus
Plasmid#168487PurposeMammalian expression of the E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe E12,14A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K22,24A)-mVenus
Plasmid#168484PurposeMammalian expression of the K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K22,24A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (K8,9A)-mVenus
Plasmid#168483PurposeMammalian expression of the K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe K8,9A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3_PKA-CαN (G2A)-mVenus
Plasmid#168482PurposeMammalian expression of the G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus.DepositorInsertthe G2A mutant of the N-terminal 47 residues of mouse PKA catalytic subunit α C-terminally tagged by monomeric Venus
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
p8115 LentiCRISPR v2 sgPTPN14-3
Plasmid#163314PurposeExpresses Cas9 and a human PTPN14-targeting control guide RNADepositorInsertsgPTPN14-3
UseCRISPR and LentiviralTagsExpressionMutationPromoterU6Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGST-Fah_S
Plasmid#163088Purposeexpressing mouse swingshift mutant FAH in bacterial cellsDepositorInsertFah_S
UseTagsGSTExpressionBacterialMutationPromoterT7Available sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Fah_S
Plasmid#163086PurposeSwingshift mutant FAH in mammalian cellsDepositorInsertsFah_S
Fah_S
UseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAM1160c2
Plasmid#159724PurposeExpress MPGIL-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MPGIL-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1674
Plasmid#159726PurposeExpress MPTLV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MPTLV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAM1479a3
Plasmid#159725PurposeExpress MYITV-G6-Dhfr in yeast two hybrid plasmid, pGBKCgDepositorInsertmDHFR (Dhfr Mouse)
UseTagsGal4DBD and MYITV-GGGGGGExpressionYeastMutationremove start and stop codon of DhfrPromoterADH1Available sinceOct. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Myosin7B-sgRNA
Plasmid#154861PurposeLentiviral expression of Cas9 and sgRNA targeting Myosin7BDepositorInsertMYO7B sgRNA (MYO7B Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJuly 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-16a-dendra2
Plasmid#105434Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-16a Dendra2 (mir16a Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-23a-2-dendra2
Plasmid#105435Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-23a-2 Dendra2 (mir23a-2 Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-26b-dendra2
Plasmid#105436Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-26b Dendra2 (mir26b Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-27b-dendra2
Plasmid#105437Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-27b Dendra2 (mir27b Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-132-2-dendra2
Plasmid#105439Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-132-2 Dendra2 (mir132-2 Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-152-dendra2
Plasmid#105440Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-152 Dendra (mir152 Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-181c-dendra2
Plasmid#105441Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-181c Dendra2 (mir181c Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
tol2-lyzC-miR-182-dendra2
Plasmid#105442Purposeoverexpression of microRNA and Dendra2 in ZebrafishDepositorInsertmiR-182 Dendra2 (mir182 Zebrafish)
UseZebrafishTagsN/AExpressionMutationPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only