Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

We narrowed to 2,493 results for: D10

Clear keywords
Showing: 1601 - 1620 of 2493 results

  6. pENTR1A mKate2-hu_ncOGT 5N5A

    Plasmid
    #154285
    Purpose
    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; 5N5A variant, no HCF-1 cleavage)
    Depositor
    Suzanne Walker
    Publication
    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Gateway Cloning
    Tags
    2x FLAG and mKate2
    Mutation
    N322A, N356A, N390A, N424A, N458A mutations (HCF-…
    Available since
    Nov. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    154285-plasmid-map-sequence-id-304861

    Enlarge

  7. pORFE0002

    Plasmid
    #112071
    Purpose
    AtCAS9 nickase D10A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 nickase D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112071-plasmid-map-sequence-id-217629

    Enlarge

  8. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Available since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

    Enlarge

  11. pYPQ265K

    Plasmid
    #173997
    Purpose
    Gateway entry clone (attL1 & attR5) UNG-rAPOBEC1(R33A)-zCas9(D10A) for C-G base editing
    Depositor
    Yiping Qi
    Publication
    Sretenovic et al Front Genome Ed. 2021 Sep 15;3:756766. d
    Insert
    UNG-rAPOBEC1(R33A)-zCas9(D10A)
    Use
    CRISPR
    Tags
    3X FLAG, NLS
    Expression
    Plant
    Mutation
    R33A in rAPOBEC1 and D10A in zCas9
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    173997-plasmid-map-sequence-id-343409

    Enlarge

  13. Lenti-EF1alpha-dCas9-VP64-MS2-VP64-ZsG

    Plasmid
    #192665
    Purpose
    3rd generation lenti vector encoding dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1
    Depositor
    Jeffrey Miner
    Insert
    dCas9-VP64, MS2-VP64, ZsGreen1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A and N863A in Cas9
    Promoter
    EF1a
    Available since
    Dec. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    192665-plasmid-map-sequence-id-379871

    Enlarge

  14. pGBW-m4095421

    Plasmid
    #149524
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 nsp3
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;6xHIS
    Expression
    Bacterial
    Mutation
    d1-745 d1063-1945
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 14, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149524-plasmid-map-sequence-id-307625

    Enlarge

  16. pX330-Flag-dHeFSpCas9

    Plasmid
    #92116
    Purpose
    Expression plasmid for human codon-optimized dead/inactive increased fidelity HeFSpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive HeFSpCas9 with FLAG tag
    Use
    CRISPR
    Tags
    3xFLAG and NLS
    Expression
    Mammalian
    Mutation
    D10A, N497A, R661A, Q695A, H840A, K848A, Q926A, K…
    Promoter
    Cbh
    Available since
    Oct. 11, 2017
    Availability
    Academic Institutions and Nonprofits only
    92116-plasmid-map-sequence-id-177904

    Enlarge

  17. pORFE1052

    Plasmid
    #112078
    Purpose
    Overexpression of the AtCAS9 nickase in plant under 2x35S promoter
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A
    Use
    CRISPR
    Expression
    Plant
    Mutation
    Plant codon optimized nCas9 D10A S. pyogenes
    Promoter
    2x35S
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    112078-plasmid-map-sequence-id-217613

    Enlarge

Showing: 1601 - 1620 of 2493 results