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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 165,736 results for: addgene

Showing: 1601 - 1620 of 165736 results

  1. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid
    #118158
    Purpose
    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS
    Depositor
    Jorge Ferrer
    Insert
    KRAB-dCas9-2A-BlastR
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Expression
    Mammalian
    Promoter
    EF1a core and U6
    Available Since
    April 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118158-plasmid-map-sequence-id-240404

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  2. MAPK3

    Plasmid
    #73235
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    MAPK3 (MAPK3 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00069:Pkinase 42-330
    Promoter
    T7
    Available Since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73235-plasmid-map-sequence-id-137225

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  4. pACλCI

    Plasmid
    #53730
    Purpose
    PlacUV5-directed synthesis of the λCI protein; used as two-hybrid control. For cloning purposes, see Addgene plasmid 53733.
    Depositor
    Ann Hochschild
    Article
    Dove et al Nature. 1997 Apr 10;386(6625):627-30.
    Insert
    bacteriophage lambda CI protein
    Expression
    Bacterial
    Promoter
    lac UV5
    Available Since
    June 9, 2014
    Availability
    Academic Institutions and Nonprofits only
    53730-plasmid-map-sequence-id-83908

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  5. p-dCas9-G9a-Hygro

    Plasmid
    #104408
    Purpose
    transient expression of dCas9-G9a fusion protein
    Depositor
    Stephan Beck
    Insert
    G9a (EHMT2 Human)
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV promoter
    Available Since
    April 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    104408-plasmid-map-sequence-id-204469

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  7. 5TEY (METTL3)

    Plasmid
    #101892
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL3 (METTL3 Human)
    Use
    Baculovirus expression
    Tags
    6xHis, TEV
    Promoter
    polyhedrin
    Available Since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101892-plasmid-map-sequence-id-194730

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  8. 4IKP

    Plasmid
    #60080
    Purpose
    Baculovirus expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    CARM1:JMC049-A06:C226836 (CARM1 Human)
    Use
    Baculovirus expression
    Tags
    6xHis
    Mutation
    See comments
    Promoter
    polyhedrin
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pBabe puro IkB alpha M

    Plasmid
    #12332
    Depositor
    Inder Verma
    Insert
    IkB alpha M (Nfkbia Mouse)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    Dominant-negative mutant. Many signal transductio…
    Available Since
    Aug. 17, 2006
    Availability
    Academic Institutions and Nonprofits only

  11. XLone-BSD SARS-CoV2 N P2A mCherry

    Plasmid
    #154398
    Purpose
    PiggybacTransposon-based tunable and temporal expression control of SARS-CoV2 N and mCherry
    Depositor
    Xiaoping Bao
    Insert
    SARS-CoV2 N Protein (N SARS-CoV2)
    Use
    Piggybac
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    TRE3G
    Available Since
    June 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    154398-plasmid-map-sequence-id-304591

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  14. pMSCV-Flag-Mm Ezh2 Y641F

    Plasmid
    #80077
    Purpose
    Retroviral expression of mouse EZH2 Y641F mutant, hygromycin selection
    Depositor
    Phillip Abbosh
    Inserts
    Flag-EZH2 Y641F (Ezh2 Mouse)
    Hygromycin phosphotransferase
    Use
    Retroviral
    Tags
    5' FLAG
    Expression
    Mammalian
    Mutation
    Y641F, R342R, N393N
    Available Since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    80077-plasmid-map-sequence-id-151639

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  15. CFL1 (CFL1A-c002)

    Plasmid
    #98237
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    CFL1A (CFL1 Human)
    Tags
    His-6-TEV
    Expression
    Bacterial
    Mutation
    pfam00241:Cofilin_ADF 27-148
    Promoter
    T7
    Available Since
    Sept. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    98237-plasmid-map-sequence-id-193416

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  17. pLenti-dSaCas9-KRAB-gRNA-TRE-blast

    Plasmid
    #201151
    Purpose
    Lentiviral expression of S. aureus dead Cas9 (dCas9/dSaCas9/SadCas9) fused to the KRAB domain as well as a gRNA targeting the tight TRE promoter and the blasticidin resistance gene.
    Depositor
    Moshe Szyf
    Inserts
    SadCas9-KRAB
    gRNA-TRE
    Use
    CRISPR and Lentiviral
    Tags
    HA
    Mutation
    gRNA sequence: ATCAGTGATAGAGAACGTATG
    Available Since
    July 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    201151-plasmid-map-sequence-id-401792

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  19. KLHL3 (KLHL3A-c003)

    Plasmid
    #110251
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    KLHL3 (KLHL3 Human)
    Tags
    His-TEV
    Expression
    Bacterial
    Promoter
    T7 - lacO
    Available Since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    110251-plasmid-map-sequence-id-212338

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Showing: 1601 - 1620 of 165736 results