Skip to main content
Addgene
Showing: 3241 - 3280 of 79739 results

  1. PIP2-CFP(UST) sensor

    Plasmid
    #170357
    Purpose
    PH-CFP(UST) fusion used to measure realtime changes in PIP2 levels.
    Depositor
    Kees Jalink
    Insert
    PH-CFP(UST) (PLCD1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    170357-plasmid-map-sequence-id-336129

    Enlarge

  2. PIP2-YFP(UST) sensor

    Plasmid
    #170358
    Purpose
    PH-YFP(UST) fusion used to measure realtime changes in PIP2 levels.
    Depositor
    Kees Jalink
    Insert
    PH-YFP(UST) (PLCD1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    170358-plasmid-map-sequence-id-335511

    Enlarge

  3. IFP1.4-H2B-6

    Plasmid
    #56269
    Purpose
    Localization: Nucleus/Histones, Excitation: 684, Emission: 708
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    IFP1.4
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    April 28, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56269-plasmid-map-sequence-id-320809

    Enlarge

  4. TBXTA-c006

    Plasmid
    #139755
    Purpose
    For protein expression/crystallography (PDB:6F59)
    Depositor
    Opher Gileadi
    Insert
    TBXT, DNA-binding domain, G177D (TBXT Human)
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    G177D; contains only amino acids E41-S224
    Promoter
    T7
    Available since
    May 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    139755-plasmid-map-sequence-id-296509

    Enlarge

  5. mCherry-PTK2-N-10

    Plasmid
    #55123
    Purpose
    Localization: Focal Adhesion Kinase, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    PTK2 (PTK2 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  6. SpCas9_hGEM(1-110)_hSLBP(56-108)

    Plasmid
    #191388
    Purpose
    SpCas9 fused with geminin and SLBP degrons to improve HDR ratio
    Depositor
    Yannick Doyon
    Insert
    hGEM(1-110)_hSLBP(56_108)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    191388-plasmid-map-sequence-id-378110

    Enlarge

  7. RAB14

    Plasmid
    #25519
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    RAB14 (RAB14 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 11, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  9. 5KKP (PUS7)

    Plasmid
    #101878
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PUS7 (PUS7 Human)
    Use
    Tags
    6xHis, TEV
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 30, 2018
    Availability
    Industry, Academic Institutions, and Nonprofits
    101878-plasmid-map-sequence-id-194704

    Enlarge

  10. mCherry-Endo-14

    Plasmid
    #55040
    Purpose
    Localization: Endosomes, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    Endo (RHOB Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Encodes amino acids 5-196 (end) of NM_004040.2
    Promoter
    CMV
    Available since
    Oct. 7, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. mCherry-H2B-18

    Plasmid
    #55055
    Purpose
    Localization: Nucleus/Histones, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Sept. 17, 2014
    Availability
    Academic Institutions and Nonprofits only
    55055-plasmid-map-sequence-id-274822

    Enlarge

  12. pT7-V5-SBP-C1-HsUpf1-siRNAres_P

    Plasmid
    #147196
    Purpose
    Mammalian Expression of HsUpf1-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsUpf1-siRNAres (UPF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    5 non silent mutations A69S, E167G, K282R, A442G…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147196-plasmid-map-sequence-id-429667

    Enlarge

  13. PS-CFP2-Tubulin-6

    Plasmid
    #57251
    Purpose
    Localization: Microtubules, Excitation: 400 / 490, Emission: 468 / 511
    Depositor
    Michael Davidson
    Insert
    NM_006082.2 (TUBA1B Human)
    Use
    Tags
    PS-CFP2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 1, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  14. pcDNA3.1-MS2-HA-HsMex3B_AD

    Plasmid
    #148478
    Purpose
    Mammalian Expression of HsMex3B
    Depositor
    Elisa Izaurralde
    Insert
    HsMex3B (MEX3B Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148478-plasmid-map-sequence-id-409989

    Enlarge

  15. SPHK1

    Plasmid
    #73260
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SPHK1 (SPHK1 Human)
    Use
    Tags
    His-6-TEV
    Expression
    Insect
    Mutation
    Promoter
    Polyhedrin
    Available since
    April 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    73260-plasmid-map-sequence-id-137346

    Enlarge

  16. PPIC

    Plasmid
    #25606
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PPIC (PPIC Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  17. g5s pD42

    Plasmid
    #117452
    Purpose
    Expression of Cyth2
    Depositor
    Richard Kahn
    Insert
    Cyth2 (CYTH2 Human)
    Use
    Tags
    V5-His6
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Nov. 1, 2018
    Availability
    Academic Institutions and Nonprofits only
    117452-plasmid-map-sequence-id-226811

    Enlarge

  18. Clathrin-15

    Plasmid
    #58190
    Purpose
    Localization: Clathrin Vesicles, Excitation: N/A, Emission: N/A
    Depositor
    Michael Davidson
    Insert
    Clathrin (CLTB Human)
    Use
    Tags
    SNAP tag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  19. pHH0103 FNBP3 WW domain #1

    Plasmid
    #104233
    Purpose
    Bacterial expression of WW domain #1 from FNBP3
    Depositor
    Marius Sudol, Sachdev Sidhu
    Insert
    FNBP3 WW domain #1 (PRPF40A Human)
    Use
    Tags
    GST
    Expression
    Bacterial
    Mutation
    codon optimized for expression in bacteria
    Promoter
    tac
    Available since
    March 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    104233-plasmid-map-sequence-id-204803

    Enlarge

  20. tdTomato-H2B-C-10

    Plasmid
    #58102
    Purpose
    Localization: Nucleus/Histones, Excitation: 554, Emission: 581
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    tdTomato
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Dec. 5, 2014
    Availability
    Academic Institutions and Nonprofits only

  21. mEos2-Tubulin-6

    Plasmid
    #57431
    Purpose
    Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  22. 4MVT

    Plasmid
    #60101
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIAS3:YTC008-B10:C225910 (PIAS3 Human)
    Use
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  23. pcDNA3.1-MS2-HA-HsMex3C_AD

    Plasmid
    #148481
    Purpose
    Mammalian Expression of HsMex3C
    Depositor
    Elisa Izaurralde
    Insert
    HsMex3C (MEX3C Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L11P in Mex3C
    Promoter
    Available since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148481-plasmid-map-sequence-id-412787

    Enlarge

  24. R777-E228 Hs.RASA1-nostop

    Plasmid
    #70512
    Purpose
    Gateway ORF clone of human RASA1 [NM_002890.2] without stop codon (for C-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASA1 (RASA1 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70512-plasmid-map-sequence-id-125961

    Enlarge

  25. pLJM60

    Plasmid
    #34610
    Depositor
    David Sabatini
    Insert
    deptor (DEPTOR Human)
    Use
    Lentiviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 1, 2012
    Availability
    Academic Institutions and Nonprofits only

  26. CMV-H2B-sfGFP-T2A-iCre

    Plasmid
    #183351
    Purpose
    Histone H2B-sfGFP and iCre recombinase
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    183351-plasmid-map-sequence-id-360158

    Enlarge

  27. hKv2.1-V5

    Plasmid
    #113056
    Purpose
    Expresses human Kv2.1 in mammalian cells
    Depositor
    Federico Sesti
    Insert
    human Kv2.1 (KCNB1 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 31, 2018
    Availability
    Academic Institutions and Nonprofits only
    113056-plasmid-map-sequence-id-217317

    Enlarge

  28. pT7-EGFP-C1-HsYTHDF1_AB

    Plasmid
    #148307
    Purpose
    Mammalian Expression of HsYTHDF1
    Depositor
    Elisa Izaurralde
    Insert
    HsYTHDF1 (YTHDF1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    148307-plasmid-map-sequence-id-381957

    Enlarge

  29. Dendra2-Tubulin-6

    Plasmid
    #57742
    Purpose
    Localization: Microtubules, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    Dendra2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 6, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  30. mAzami Green-H2B

    Plasmid
    #56405
    Purpose
    Localization: Nucleus/Histones, Excitation: 492, Emission: 505
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mAzami Green
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 29, 2015
    Availability
    Academic Institutions and Nonprofits only
    56405-plasmid-map-sequence-id-194984

    Enlarge

  31. mEos3.2-Vinculin-N-21

    Plasmid
    #66952
    Purpose
    Localization: Focal Adhesions, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Vinculin (VCL Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    V234L in Vinculin
    Promoter
    CMV
    Available since
    July 7, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  32. PANK1

    Plasmid
    #25517
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PANK1 (PANK1 Human)
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 24, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  34. YPet-H2B-6

    Plasmid
    #56632
    Purpose
    Localization: Histone H2B, Excitation: 513, Emission: 527
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    YPet
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Dec. 19, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  35. DsRed2-H2B-6

    Plasmid
    #55837
    Purpose
    Localization: Nucleus/Histones, Excitation: 558, Emission: 583
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    DsRed2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  36. mCherry-H2B-C-10

    Plasmid
    #55057
    Purpose
    Localization: Nucleus/Histones, Excitation: 587, Emission: 610
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Use
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available since
    Aug. 29, 2014
    Availability
    Academic Institutions and Nonprofits only

  37. IDG_TMEM38B_OE_1

    Plasmid
    #161658
    Purpose
    Overexpresses TMEM38B
    Depositor
    Mike McManus
    Insert
    TMEM38B (TMEM38B Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    161658-plasmid-map-sequence-id-319639

    Enlarge

  38. mEos3.2-Rad21-C-18

    Plasmid
    #57481
    Purpose
    Localization: Centromere, Excitation: 507 / 572, Emission: 516 / 580
    Depositor
    Michael Davidson
    Insert
    Rad21 (RAD21 Human)
    Use
    Tags
    mEos3.2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 5, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  39. mAzurite-Tubulin-6

    Plasmid
    #55233
    Purpose
    Localization: Microtubules, Excitation: 384, Emission: 450
    Depositor
    Michael Davidson
    Insert
    Tubulin (TUBA1B Human)
    Use
    Tags
    mAzurite
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  40. mEmerald-Cofilin-C-10

    Plasmid
    #54047
    Purpose
    Localization: Actin, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    NM_005507.2 (CFL1 Human)
    Use
    Tags
    mEmerald
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 2, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
Showing: 3241 - 3280 of 79739 results