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Showing: 1621 - 1640 of 1801 results

  3. pDULE-ABK

    Plasmid
    #49086
    Purpose
    A pyrrolysyl tRNA/tRNA sythetase pair evolved to efficiently incorporate an aliphatic diazirine amino acid into proteins in E. coli and mammalian cells for crosslinking experiments
    Depositor
    Peter Schultz
    Article
    Ai et al Chembiochem. 2011 Aug 16;12(12):1854-7.
    Inserts
    tRNA synthetase
    tRNA for a photocrosslinking amino acid
    Use
    Tags
    Expression
    Bacterial
    Mutation
    L274M, C313A, Y349F
    Promoter
    GlnRS and lpp
    Available since
    Nov. 6, 2013
    Availability
    Academic Institutions and Nonprofits only
    49086-plasmid-map-sequence-id-396431

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  5. pET28a Gx-mNG-NL

    Plasmid
    #169106
    Purpose
    Expresses protein G fused to mNeonGreen and NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - mNeonGreen - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169106-plasmid-map-sequence-id-335361

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  9. pET28a-B4E

    Plasmid
    #162067
    Purpose
    For expresion of the B4E fusion protein, consists of the ampR gene (encoding the enzyme β-lactamase without the native signal sequence) fused to residues 28-215 of the murine eIF4E
    Depositor
    Karen Polizzi
    Article
    Moya-Ramirez et al Nucleic Acids Res. 2020 Dec 16;48(22):e1
    Insert
    ampR-eIF4E fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    eIF4E incorporating the K119A mutation, which is …
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162067-plasmid-map-sequence-id-325536

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  10. pMA_Int

    Plasmid
    #110096
    Purpose
    E. coli replicative vector which contains the mycophage integrase gene required for the attP/attB integration. Is co-transformed in trans together with pFLAG_attP, but cannot replicate in mycobacteria, and is thus lost shortly after electroporation.
    Depositor
    Markus Seeger
    Article
    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.
    Insert
    mycophage integrase
    Use
    Required for transformation of attb integrating v…
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    110096-plasmid-map-sequence-id-211299

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  11. pET28a Gx-NL

    Plasmid
    #169105
    Purpose
    Expresses protein G fused to NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169105-plasmid-map-sequence-id-335658

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  12. pRSF-G1mCNPRS

    Plasmid
    #174718
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of L-3-(2-cyano-4-pyridyl)alanine (mCNP), into proteins in E. coli in response to the amber (TAG) codon
    Depositor
    Thomas Huber
    Article
    Abdelkader et al Angew Chem Int Ed Engl. 2022 Jan 31. doi
    Insert
    tRNA synthetase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    174718-plasmid-map-sequence-id-346623

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  13. pMS_366

    Plasmid
    #182131
    Purpose
    expresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutation
    Depositor
    George Church
    Article
    Schubert et al Proc Natl Acad Sci U S A. 2021 May 4;118
    Inserts
    retron RNA
    ec.86 RT
    CspRecT
    Use
    Tags
    Expression
    Mutation
    incorporated rifampicin-resistance-conferring don…
    Promoter
    Available since
    Sept. 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    182131-plasmid-map-sequence-id-355782

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  14. pET28a Gx-NL3

    Plasmid
    #169108
    Purpose
    Expresses protein G fused to 3 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only
    169108-plasmid-map-sequence-id-335659

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  15. pET28a Gx-NL2

    Plasmid
    #169107
    Purpose
    Expresses protein G fused to 2 copies of NanoLuc. The protein G domain contains an amber stop-codon at position 24 for incorporation of the unnatural amino acid para-benzoylphenylalanine.
    Depositor
    Maarten Merkx
    Article
    Wouters et al Bioconjug Chem. 2020 Mar 18;31(3):656-66
    Insert
    protein G - NanoLuc - NanoLuc
    Use
    Tags
    his-tag and strep-tag
    Expression
    Bacterial
    Mutation
    Ala 24 stop
    Promoter
    T7
    Available since
    June 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    169107-plasmid-map-sequence-id-334716

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  17. pDEST-pSP172BSSPE-Swa1::HA-RfA

    Plasmid
    #186404
    Purpose
    Destination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.
    Depositor
    Patrick Lemaire
    Article
    Roure et al PLoS One. 2007. 2(9):e916.
    Type
    Empty backbone
    Use
    Gateway destination vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    186404-plasmid-map-sequence-id-369072

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Showing: 1621 - 1640 of 1801 results