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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,492 results for: PUC19

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Showing: 1621 - 1640 of 2492 results

  1. P530_SIX6-p2A-h2b-EGFP

    Plasmid
    #239101
    Purpose
    Human donor plasmid for integration of p2A-h2b-EGFP into the 3' end of SIX6. This includes the donor casette and a U6 driven guide sequence to be used with Cas9.
    Depositor
    Karl Wahlin
    Article
    Wahlin et al Front Cell Dev Biol. 2021 Nov 16;9:76472
    Insert
    SIX6 (SIX6 Human)
    Use
    Donor plasmid
    Tags
    p2A-h2b-EGFP
    Expression
    Mammalian
    Promoter
    Endogenous SIX6 promoter
    Available Since
    June 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    239101-plasmid-map-sequence-id-474411

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  2. qTAG-C-mStayGold-Puro-H2BC11-MMEJ

    Plasmid
    #227333
    Purpose
    MMEJ Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    227333-plasmid-map-sequence-id-452137

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  3. qTAG-N-Puro-mScarlet-ACTB

    Plasmid
    #207754
    Purpose
    Donor template for Puro-2A-mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Puro-mScarlet Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 16, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207754-plasmid-map-sequence-id-415028

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  4. qTAG-N-Blast-mNeon-ACTB

    Plasmid
    #207751
    Purpose
    Donor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    ACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207751-plasmid-map-sequence-id-416864

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  5. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

    Plasmid
    #207760
    Purpose
    MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 15, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207760-plasmid-map-sequence-id-413789

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  7. pYI2165

    Plasmid
    #228347
    Purpose
    2,4-Diacetylphloroglucinol (DAPG)-regulatable ALX-0171 expression
    Depositor
    Jun Ishii
    Article
    Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi
    Insert
    MFalpha(2xAdv)-LE-ALX-0171-3A-FLAG-His
    Use
    Affinity Reagent/ Antibody
    Tags
    AAA linker followed by FLAG-His6 and MF_ secretio…
    Expression
    Yeast
    Promoter
    2,4-Diacetylphloroglucinol-regulatable synthetic …
    Available Since
    March 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228347-plasmid-map-sequence-id-457108

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  8. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid
    #176264
    Purpose
    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2
    Depositor
    John van der Oost
    Article
    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-
    Insert
    dead spCas9
    Use
    CRISPR, Luciferase, and Synthetic Biology ; Expre…
    Tags
    Kruppel associated box domain and luciferase
    Mutation
    H840A and D10A mutations on spCas9 to inactivate …
    Available Since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176264-plasmid-map-sequence-id-347050

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  10. qTAG-C-moxGFP-Puro-H3C2

    Plasmid
    #207783
    Purpose
    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    H3C2 Homology Arms flanking a moxGFP-Puro Cassette (H3C2 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207783-plasmid-map-sequence-id-416185

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  14. pMLX-donor

    Plasmid
    #112370
    Purpose
    CRISPR donor plasmid to tag human transcription factor MLX with GFP
    Depositor
    Kevin White
    Insert
    MLX homology arms flanking EGFP-IRES-Neo cassette (MLX Human)
    Use
    CRISPR
    Mutation
    hg38:17:42570798:G>A, hg38:17:42570950:T>G,…
    Available Since
    Dec. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112370-plasmid-map-sequence-id-229723

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  18. pDmut06

    Plasmid
    #65945
    Purpose
    Theophylline riboswitch D mut derivatives 06
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Expression
    Bacterial
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only

  19. pDmut10

    Plasmid
    #65946
    Purpose
    Theophylline riboswitch D mut derivatives 10
    Depositor
    Justin Gallivan
    Insert
    luciferase
    Expression
    Bacterial
    Available Since
    Sept. 3, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 1621 - 1640 of 2492 results