We narrowed to 2,807 results for: cdna expression plasmid
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Plasmid#170343Purpose6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsert6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pVH234-Tier1-PmPGK1-tTA
Plasmid#169574PurposeTier-1 vector encoding PmPGK1-driven tTA expression (PmPGK1-tTA-pA).DepositorInserttetracycline-controlled transactivator
UseTagsExpressionMammalianMutationPromoterPPGKAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_puromycin
Plasmid#118595PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
CDK1as_T2A_Zeo
Plasmid#118596PurposeOne of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and Zeocin resistance cassette flanked by Sleeping Beauty transposon.DepositorInsertCDK1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLXSN IkB alpha M
Plasmid#12330DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorInsertSAR1 (SAR1A Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SAR1-V5-His6
Plasmid#67451PurposeMammalian expression of hSar1 with C-term V5 and His6 tagDepositorInsertSAR1 (SAR1A Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SARA-GFP
Plasmid#67410PurposeMammalian expression of hSara with C-term GFP tagDepositorInsertSARA (SAR1B Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-SARA-V5-His6
Plasmid#67452PurposeMammalian expression of hSara with C-term V5 and His6 tagDepositorInsertSARA (SAR1B Human)
UseTagsHis and V5ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
HA-hDAGLb-V5
Plasmid#87675PurposeExpresses human DAGLb protein with a HA tag inserted in the first extracellular loop, and intracellular V5 tag located on the C-terminalDepositorInsertHuman Diacylglycerol Lipase Beta (DAGLB Human)
UseTagsHA and V5 tagExpressionMammalianMutationHemagglutinin (HA) peptide sequence (YPYDVPDYA) f…PromoterT7Available sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVH254-Tier1-OTetO7-Pmin-SEAP
Plasmid#169585PurposeTier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-Pmin-SEAP-pA).DepositorInserttetracycline-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pVH229-Tier1-OTtgO2-PCMVmin2-SEAP
Plasmid#169565PurposeTier-1 vector encoding PTtgO2-driven SEAP expression (OTtgO2-PCMVmin-2-SEAP-pA).DepositorInsertphloretin-controlled SEAP expression
UseTagsExpressionMammalianMutationPromoterTtgO2-PCMVminAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTS2364-Tier1-IRES
Plasmid#169562PurposeTier-1 vector carrying an internal ribosome entry site (IRES-pA)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_K95M
Plasmid#108106PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & kinase-dead mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221E
Plasmid#108108PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TE mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_Sik3_T142Q
Plasmid#119973PurposeLentiviral expression plasmid of mouse Sik3 cDNA (gatekeeper mutation) with neomycin resistance geneDepositorInsertSik3 (Sik3 Mouse)
UseLentiviralTagsExpressionMammalianMutationchange Threonine 142 to GlutaminePromoterEFS promoterAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
(p)odr-3::GFP::EGL-4
Plasmid#100897PurposeGFP and the EGL-4 cDNA driven by the odr-3 promoter, which expresses in the AWA, AWB, and AWC olfactory sensory neurons in C. elegans. unc-54 3'UTR in sequence as well.DepositorInsert56-2741: odr-3 promoter
UseTagsExpressionWormMutationPromoterodr-3Available sinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T221A
Plasmid#108107PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & TA mutation at LKB1 phosphorylation site) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
attB-mCh-Poly(A)‐NeoR
Plasmid#65528PurposeNeoR containing plasmid for the generation of gene disruptions via Bxb1-mediated recombination into MIN-tagged cell lines. This vector can also be used to express cDNAs.DepositorInsertattB-mCherry
UseMouse Targeting; Bxb1TagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
LentiV_Neo_SIK3_CR_T142Q
Plasmid#108109PurposeLentiviral expression plasmid of human SIK3 cDNA (CRISPR-resistant silent mutation & gatekeeper mutation) with neomycin resistance geneDepositorInsertSIK3 (SIK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationchange guanine 501 to cytosine (silent mutation),…PromoterEFS promoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only