We narrowed to 4,520 results for: gca

1. pegRNA-GFP>BFP_Y66H-NGG

   Plasmid

   #185480

   Purpose

   pegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.

   Depositor

   Sabine Fuchs

   Article

   Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

   Insert

   GFP>BFP_Y66H-pegRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLKO.1-shCELF4-4405

   Plasmid

   #115466

   Purpose

   Constitutive lentiviral expression

   Depositor

   William Hahn

   Article

   Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

   Insert

   shCELF4-4405 (CELF4 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPN440

   Plasmid

   #137874

   Purpose

   PiggyBac vector for expression of 3xgRNA targeting TCF4 for CRISPRa; mRFP-T2A-BlasticidinR

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

   Insert

   TCF4 (TCF4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. RPS6KA3 gRNA (BRDN0001148452)

   Plasmid

   #75943

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human RPS6KA3

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   RPS6KA3 (RPS6KA3 Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. CAMKV gRNA (BRDN0001145768)

   Plasmid

   #75526

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human CAMKV

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   CAMKV (CAMKV Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. FN3K gRNA (BRDN0001148047)

   Plasmid

   #77433

   Purpose

   3rd generation lentiviral gRNA plasmid targeting human FN3K

   Depositor

   David Root, John Doench

   Article

   Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

   Insert

   FN3K (FN3K Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. TP53-KO_gRNA_2

   Plasmid

   #195131

   Purpose

   TP53-targeting gRNA in the LRG2.1 backbone

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   TP53 KO gRNA 2 (TP53 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p426_Cas9_gRNA-R-ARS607c

   Plasmid

   #87409

   Purpose

   p426_Cas9_gRNA-ARS607c without ribozyme All-in-one CRISPR plasmid for integrating, deleting, or replacing a gene in S. cerevisiae. High-copy, URA3 plasmid contains human codon-optimized Cas9 and a guide RNA targeting ARS607c sequence CTATTTTTGCTTTCTGCACA in yeast chromosome 6.

   Depositor

   Aindrila Mukhopadhyay

   Article

   Reider Apel et al Nucleic Acids Res. 2017 Jan 9;45(1):496-

   Insert

   Human Optimized S. pyogenes Cas9 and guide RNA targeting ARS607c
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   ADH1, pTyrosine

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

   Plasmid

   #113697

   Purpose

   U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

   Depositor

   Jonathan Ploski

   Article

   Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

   Inserts

   SaCas9 gRNA Cassete
   GFP
   

   Use

   AAV and CRISPR

   Tags

   KASH

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. DYRK2 gRNA (BRDN0001149160)

    Plasmid

    #77537

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYRK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. DYRK2 gRNA (BRDN0001147259)

    Plasmid

    #77536

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human DYRK2

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pU6-crRNA(TBK1)

    Plasmid

    #109322

    Purpose

    AAV vector expressing crRNA for AsCpf1 (RR variant) targeting human TBK1

    Depositor

    Feng Zhang

    Article

    Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

    Insert

    mCherry-KASH (TBK1 Human)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(hXIAP_2b)-PGKpuro2ABFP-W

    Plasmid

    #208421

    Purpose

    Lentiviral gRNA plasmid targeting human XIAP gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Cancer Res Commun. 2024 Nov 1;4(11):2919

    Insert

    XIAP (XIAP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgNF2

    Plasmid

    #229433

    Purpose

    knockout of NF2

    Depositor

    Christopher Vakoc

    Article

    Klingbeil et al Cancer Discov. 2024 Jul 26. doi: 10.1158

    Insert

    sgNF2 (NF2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6;bU6;EFS

    Available since

    Jan. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid

    #226992

    Purpose

    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b

    Depositor

    Benjamin Kleinstiver

    Article

    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.

    Insert

    SpCas9 gRNA 2b to create OPA1-del_exon5
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(PDLIM1_5-1)-PGKpuroBFP-W

    Plasmid

    #211978

    Purpose

    Express gRNA against PDLIM1 with puro and BFP

    Depositor

    Kosuke Yusa

    Article

    Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

    Insert

    sgRNA targeting PDLIM1 (PDLIM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6 promoter

    Available since

    Jan. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. LENTICRISPR-ENO1_sgRNA1

    Plasmid

    #201592

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Vamsi Mootha, Alexis Jourdain

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    ENO1 (ENO1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pXPR_BRD003 LIPT2-2

    Plasmid

    #184483

    Purpose

    Lentivirus gRNA targeting human LIPT2 gene

    Depositor

    Todd Golub, Peter Tsvetkov

    Article

    Tsvetkov et al Science. 2022 Mar 18;375(6586):1254-1261

    Insert

    LIPT2 (LIPT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. IP6K3 gRNA (BRDN0001147058)

    Plasmid

    #77654

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human IP6K3

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    IP6K3 (IP6K3 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. ADCK1 gRNA (BRDN0001149367)

    Plasmid

    #76087

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human ADCK1

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    ADCK1 (ADCK1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge