We narrowed to 5,016 results for: puc

1. scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

   Pooled Library

   #217423

   Purpose

   scQers promoter control #4 (intermediate: Pgk1 promoter, p042)

   Depositor

   Jay Shendure

   Article

   Lalanne et al bioRxiv 2022.12.10.519236

   Expression

   Bacterial and Mammalian

   Use

   RNAi

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

2. AAV-pCbh-SpRY[C-term]-gRNAentry (CA20)

   Plasmid

   #197511

   Purpose

   Cbh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpRY and BsmBI entry cassette to clone SpCas9 gRNA spacer

   Depositor

   Benjamin Kleinstiver

   Article

   Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

   Insert

   AAV-[ITR]-pCbh-BPNLS-NpuC-SpRY[C-term]-BPNLS-gRNA[BsmBI]-pU6-[ITR]
   

   Use

   AAV and CRISPR

   Tags

   BPNLS and BPNLS-NpuC(intein)

   Expression

   Mutation

   C-terminal mutations of SpRY(L1111R/D1135L/S1136W…

   Promoter

   Cbh

   Available since

   March 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. High Complexity Golden Gate Assembly Standards Kit (24 fragment lac cassette)

   Plasmid Kit

   #1000000221

   Purpose

   A positive control for high-complexity golden gate assembly. Junction sites were predicted using comprehensive end-joining ligation fidelity and bias data to allow high accuracy assembly.

   Depositor

   Eric Cantor

   Available since

   June 1, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

4. pHybr_r2a>Fab-srt-his

   Plasmid

   #124810

   Purpose

   HDR template to knock-in sortag and histag in rat IgG2a heavy chain locus. Use in combination with PX459-gR2A_Hinge to convert rat IgG2a hybridoma to Fab' fragment producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   rat IgG2a Hinge-G4S-Sortag-Histag
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial

   Mutation

   Promoter

   pUC

   Available since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. DinoIII-neo

   Plasmid

   #155026

   Purpose

   Dinoflagelate backbone for amino 3'-glycosyl phosphotransferase (neo) gene

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   amino 3'-glycosyl phosphotransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. DinoIII-pat

   Plasmid

   #155028

   Purpose

   Dinoflagelate backbone for Phosphinothricin N-acetyltransferase, pat, gene

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   Phosphinothricin N-acetyltransferase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. DinoIII-bsr

   Plasmid

   #155027

   Purpose

   Dinoflagelate backbone for blasticidin S-resistance gene (bsr)

   Depositor

   Senjie Lin

   Article

   Faktorova et al Nat Methods. 2020 May;17(5):481-494. doi

   Insert

   Blasticidin-S deaminase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pminTolR4-R2

   Plasmid

   #67711

   Purpose

   mini Tol2 backbone with att4-attR2 cassette

   Depositor

   Nathan Lawson

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pILGFP1DFB

   Plasmid

   #185871

   Purpose

   A parental plasmid for in vivo gene amplification at RPL25 locus

   Depositor

   Claudia Vickers

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   None

   Promoter

   Available since

   Sept. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pILGFP3AA5

    Plasmid

    #185875

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~47 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Aug. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pILGFP3AG4

    Plasmid

    #185874

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~12 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pILGFP5EA4

    Plasmid

    #185879

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (4 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pILGFP5EC4

    Plasmid

    #185880

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (9 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pILGFP5EF3

    Plasmid

    #185881

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (13 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT1004

    Plasmid

    #8613

    Depositor

    Robert Hartley

    Insert

    binase, barstar
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    as pMT1002

    Promoter

    Available since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pILGFP4BG6

    Plasmid

    #185877

    Purpose

    A parental plasmid for in vivo gene amplification (HamAmp) at SEC23 locus

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pILGFP3AE4

    Plasmid

    #185873

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (~11 copy)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pILGFP5EG3

    Plasmid

    #185878

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at SEC23 locus (1 copy reference)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pILGFP3A5C

    Plasmid

    #185872

    Purpose

    A plasmid for in vivo gene amplification (HamAmp) at RPL25 locus (1 copy reference)

    Depositor

    Claudia Vickers

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    None

    Promoter

    Available since

    Sept. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDmut06

    Plasmid

    #65945

    Purpose

    Theophylline riboswitch D mut derivatives 06

    Depositor

    Justin Gallivan

    Insert

    luciferase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 3, 2015

    Availability

    Academic Institutions and Nonprofits only