We narrowed to 32,518 results for: Eng

1. 139H2_LC

   Plasmid

   #206202

   Purpose

   For recombinant expression of the light chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells.

   Depositor

   Joost Snijder

   Article

   Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

   Insert

   anti-MUC1 antibody 139H2 light chain (Igkc Mouse)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGL3-MTS-CCDB-N136-RsDddA-A-UGI-PURO

   Plasmid

   #205463

   Purpose

   A backbone containing a RsDddA fragment A for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-A; UGI
   

   Expression

   Mammalian

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL3-MTS-CCDB-N136-RsDddA-C-UGI-PURO

   Plasmid

   #205465

   Purpose

   A backbone containing a RsDddA fragment C for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-C; UGI
   

   Expression

   Mammalian

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL3-MTS-CCDB-N136-RsDddA-B-UGI-PURO

   Plasmid

   #205464

   Purpose

   A backbone containing a RsDddA fragment B for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-B; UGI
   

   Expression

   Mammalian

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGL3-MTS-CCDB-N136-RsDddA-D-UGI-PURO

   Plasmid

   #205466

   Purpose

   A backbone containing a RsDddA fragment D for constructing mitochondria-targeted RsDdCBE expression plasmid

   Depositor

   Bin Shen

   Article

   Cheng et al Mol Ther Nucleic Acids. 2023 Sep 9;34:10

   Insert

   MTS; N136; ccdb; RsDddA-D; UGI
   

   Expression

   Mammalian

   Available Since

   Aug. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLV-PGK-dt/CBA-EGFP-NLS

   Plasmid

   #163918

   Purpose

   Contains bidirectional reporter genes dtomato and nuclear-targeting EGFP.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   dtomato and EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Added nuclear localization signal to EGFP

   Available Since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTripZ-B2

   Plasmid

   #163924

   Purpose

   For making a cell line that expresses doxycyline-inducible RNAi suppressor protein B2 for increasing titer of lentivirus with bidirectional promoters during virus production.

   Depositor

   John Engelhardt

   Article

   Choi et al Genes (Basel). 2020 Oct 6;11(10). pii: g

   Insert

   Flock house virus B2 protein (FHVs1gp2 )
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFB-U6-Chrnb4-gRNA-hSyn-mCherry-WPRE-SV40pA

   Plasmid

   #128345

   Purpose

   pAAV encoding gRNA sequence for loss-of-function indels

   Depositor

   Ryan Drenan

   Article

   Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

   Insert

   Chrnb4 (Chrnb4 Mouse)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   July 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hSyn-GRAB-HaloDA1.0

   Plasmid

   #234408

   Purpose

   Expresses the chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0 in neurons

   Depositor

   Yulong Li

   Article

   Zheng et al Science. 2025 Jun 5;388: 6751. doi: http

   Insert

   GPCR activation based chemigenetic dopamine (DA) sensor GRAB_HaloDA1.0
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   June 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. 139H2_HC

    Plasmid

    #206201

    Purpose

    For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.

    Depositor

    Joost Snijder

    Article

    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202

    Insert

    anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
    

    Tags

    -AAAHHHHHHHH

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Zhou-COVID-19-Spike

    Plasmid

    #161029

    Purpose

    Extracellular domain of SARS-CoV-2 spike protein that has been engineered so that it assembles pseudo virus more efficiently. Also contains portions of VSV-G for production of psuedovirus.

    Depositor

    Dapeng Zhou

    Article

    Tong et al Vaccines (Basel). 2021 Mar 22;9(3). pii:

    Insert

    Engineered COVID-19 Spike protein, VSV-G (S SAR-CoV-2, Vesicular stomatitis virus)
    

    Expression

    Mammalian

    Mutation

    Codon optimized

    Available Since

    Jan. 12, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. EF.delmFasL.CMV.GFP

    Plasmid

    #17620

    Depositor

    Linzhao Cheng

    Article

    Yu et al Mol Ther. 2003 Jun . 7(6):827-38.

    Insert

    delFasL (Fasl Mouse)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Noncleavable mouse delFasL . Deleted the exon 2 r…

    Available Since

    March 27, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. WP_010880027.1

    Plasmid

    #194729

    Purpose

    Lumazine synthase of the hyperthermophile Aquifex aeolicus bacterium

    Depositor

    Fang Li

    Article

    Geng et al PLoS Pathog. 2021 Sep 7;17(9):e1009897.

    Insert

    nanoparticle-proteinA and His tag
    

    Tags

    His tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-AuroraB kd K106R

    Plasmid

    #108493

    Purpose

    expression of EGFP-AuroraB kinase dead (K106R)

    Depositor

    Susanne Lens

    Article

    Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

    Insert

    AuroraB (AURKB Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Kinase death K106A

    Promoter

    CMV

    Available Since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE2-TetO-hcMYC

    Plasmid

    #206387

    Purpose

    Expresses human c-MYC in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    c-MYC (MYC Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE2-TetO-hSOX17FNV

    Plasmid

    #206384

    Purpose

    Expresses human SOX17FNV in mammalian cells, for lentivirus generation.

    Depositor

    Ralf Jauch

    Article

    Weng et al Sci Adv. 2023 Aug 25;9(34):eadh2501. doi

    Insert

    SOX17FNV (SOX17 Human)
    

    Use

    Lentiviral

    Available Since

    Oct. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLL8 lenti-D10Acas9-3xflag-blast

    Plasmid

    #112134

    Purpose

    lenti vector encoding D10Acas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-D10ACas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    D10Acas9-3xflag-blast
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D10A mutant in Cas9

    Promoter

    EF1a

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLL4 sgRNA(MS2)-MS2-hA3a

    Plasmid

    #112130

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFB-U6-Chrna5-gRNA-hSyn-mCherry-WPRE-SV40pA

    Plasmid

    #128340

    Purpose

    pAAV encoding gRNA sequence for loss-of-function indels

    Depositor

    Ryan Drenan

    Article

    Peng et al Eur J Neurosci. 2018 May 19. doi: 10.111

    Insert

    Chrna5 (Chrna5 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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