Addgene

We narrowed to 13,707 results for: TIM

Showing: 1641 - 1660 of 13707 results

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pYES2.1-pomMet8

Plasmid

#107449

Purpose

Overexpression of MET fusion protein in S. cerevisiae

Depositor

Clare O'Connor

Article

Reeves et al CBE Life Sci Educ. 2018 Spring;17(1). pi

Insert

Met8

Use

Tags

V5-His

Expression

Yeast

Mutation

Promoter

GAL1

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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SJZ7-KAN

Plasmid

#228260

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to G418

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

kan

Use

Tags

Expression

Yeast

Mutation

Promoter

GPM1 S.c.

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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SJZ4-NAT

Plasmid

#228257

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to nourseothricin

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

nat1

Use

Tags

Expression

Yeast

Mutation

Promoter

FBA1 S.c.

Available since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNano

Plasmid

#169895

Purpose

Minimal cloning vector

Depositor

Richard Schneider

Article

Chu et al Biol Open. 2020 Oct 6;9(10). pii: bio.05

Type

Empty backbone

Use

Cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-mEGFP

Plasmid

#127813

Purpose

Hetero-dimer mCherry-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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Spectrin-C-cpstFRET

Plasmid

#61111

Purpose

Expression of Spectrin-C-term cpstFRET to measure mechanical stress and report multidimensional stress fields

Depositor

Fred Sachs

Article

Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

Insert

spectrin-cpstFRET

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

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pTYB2-eIF4B

Plasmid

#37226

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pP2-P1(S')

Plasmid

#196334

Purpose

Plasmid expressing chimeric P2-P1(S') tail fiber

Depositor

Baojun Wang

Article

Fa-Arun et al ACS Synth Biol. 2023 Feb 17;12(2):596-60

Insert

chimeric tail fiber P2-P1(S') and chaperone protein U'

Use

Tags

Expression

Bacterial

Mutation

Promoter

P2 late promoter

Available since

June 6, 2023

Availability

Academic Institutions and Nonprofits only

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pFH14

Plasmid

#125799

Purpose

Level0 SaCas9, Arabidopsis codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

SaCas9, Arabidopsis codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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Cdk1-mCherry

Plasmid

#54466

Purpose

CDK1-mCherry in pIVT vector for in vitro transcription

Depositor

Michael Lampson

Article

Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

Insert

Cdk1 mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

Plasmid

#130509

Purpose

expresses mScarlet

Depositor

Dorus Gadella

Article

Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

Insert

mScarlet

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pMAL-BRT17

Plasmid

#45358

Depositor

Scott Banta

Article

Shur et al Biotechniques. 2013 Apr;54(4):197-206. d

Insert

17 Repeat Beta Roll Tag

Use

Tags

Maltose Binding Protein (MBP)

Expression

Bacterial

Mutation

Promoter

Ptac

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

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mCherry2-mEGFP

Plasmid

#127814

Purpose

Hetero-dimer mCherry2-mEGFP expression vector

Depositor

Salvatore Chiantia

Article

Dunsing et al Sci Rep. 2018 Jul 13;8(1):10634. doi: 10

Insert

mCherry2-mEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pFH47

Plasmid

#125812

Purpose

Level0 LbCas12a, human codon optimized

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

LbCas12a, human codon optimized

Use

CRISPR and Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRec ΔLacI

Plasmid

#104575

Purpose

Expresses Cas1, Cas2 under PLTetO-1 promoter, and TetR constitutively.

Depositor

Harris Wang

Article

Sheth et al Science. 2017 Dec 15;358(6369):1457-1461

Insert

PTet-cas12, tetR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal mCherry2-L

Plasmid

#104027

Purpose

Plasmid encoding 2ndVal mCherry2-L

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal mCherry2-L

Use

Low copy

Tags

Expression

Bacterial

Mutation

mCherry2-L with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-EPN-01(HiBiT)

Plasmid

#162590

Purpose

EPN-01 tagged with HiBiT tag under CMV promoter

Depositor

Masaharu Somiya

Article

Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

Insert

EPN-01

Use

Tags

HiBiT

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX4T-3-6AOptParkin

Plasmid

#165107

Purpose

bacterial expression of a GST fusion of e.coli optimized Parkin with 6 alanine linker between the GST and the inserted Parkin gene

Depositor

Arthur Haas

Insert

Parkin isoform 1 (PRKN Human)

Use

Tags

GST-6-alanine

Expression

Bacterial

Mutation

codon optimized for E. coli expression

Promoter

Available since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

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pEB2-2ndVal Katushka

Plasmid

#104029

Purpose

Plasmid encoding 2ndVal Katushka

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

2ndVal Katushka

Use

Low copy

Tags

Expression

Bacterial

Mutation

Katushka with valine inserted in 2nd position

Promoter

proC

Available since

Dec. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pET-51b-HaloTag11

Plasmid

#175519

Purpose

Expression of HaloTag11 in bacterial cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

HaloTag11

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 13, 2021

Availability

Academic Institutions and Nonprofits only

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