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pCMV myc DAF-12

Plasmid

#32566

Depositor

Al Fisher

Article

Hochbaum et al PLoS Genet. 2011 Jul;7(7):e1002179. Epub

Insert

DAF-12 (daf-12 Nematode)

Tags

myc epitope

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 20, 2011

Availability

Academic Institutions and Nonprofits only

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pCZGY3195

Plasmid

#172695

Purpose

Punc-25-gfp1-10 Expresses split GFP parts 1-10 specifically in C. elegans GABAergic motor neurons (only shows active fluorescence when binds to GFP11)

Depositor

Yishi Jin

Article

Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 1

Insert

Punc-25

Tags

GFP 1-10

Expression

Worm

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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XW21

Plasmid

#65837

Purpose

XW21 Punc-4c-QS-3'UTR-unc-54 (no SL2-mcherry)

Depositor

Kang Shen

Article

Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

Insert

Neurospora crassa OR74A repressor (qa-1S)

Expression

Worm

Promoter

Punc-4c

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

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AP625-1

Plasmid

#70051

Purpose

Expresses myc::TEV::3Xflag::eGFP::3Xflag::TEV::myc in bacteria

Depositor

Geraldine Seydoux

Article

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

eGFP

Tags

3Xflag::TEV::myc and myc::TEV::3Xflag

Expression

Bacterial

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pNH078

Plasmid

#42164

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pAP639-2F

Plasmid

#99486

Purpose

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

3XFlag::TEV::6XHis::mTurquoise::6XHis::TEV::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pISlin41m(s) (lin-41 microRNA mut reporter)

Plasmid

#11907

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence mut (lin-41 Nematode)

Use

Luciferase

Expression

Mammalian

Mutation

point mutations in miRNA complementary site

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pCFJ1666

Plasmid

#51485

Purpose

miniMos transposable element with PuroR, MCS cloning

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2014 May;11(5):529-34. doi:

Type

Empty backbone

Expression

Worm

Available Since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

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pOX-twk43

Plasmid

#16128

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (F32H5.2, twk-43 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pAWp9-5

Plasmid

#73856

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg2-U6p-GFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
pAP608-1

Plasmid

#99484

Purpose

H2B::linker::GFP (contain 3 introns)::3XFlag::TEV::Myc::tbb-2 3UTR

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

H2B::linker::GFP with introns::3XFlag::TEV::Myc::tbb-2 3UTR

Expression

Bacterial

Available Since

Sept. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pAP607-2

Plasmid

#99483

Purpose

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

Myc::TEV::3XFlag::GFP::3XFlag::TEV::Myc

Expression

Bacterial

Available Since

Sept. 5, 2017

Availability

Academic Institutions and Nonprofits only

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pPK348

Plasmid

#38145

Depositor

Patricia Kuwabara

Article

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

ptc-3::GFP (ptc-3 Nematode)

Expression

Worm

Promoter

ptc-3

Available Since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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mg182

Plasmid

#34662

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A2;B2;C1) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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pSA109

Plasmid

#59783

Purpose

drives ZIF-1 protein in C. elegans intestinal cells

Depositor

Jeremy Nance

Article

Armenti et al Development. 2014 Dec;141(23):4640-7. do

Inserts

elt-2 promoter (elt-2 Nematode)
zif-1 coding sequence (zif-1 Nematode)
unc-54 3' UTR

Available Since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pET3CTc3A(1-135)

Plasmid

#63138

Purpose

Expression of human Tc3(1–135) bipartite domain tagged with 6xHis

Depositor

Titia Sixma

Article

Watkins et al Nucleic Acids Res. 2004 Aug 10;32(14):43

Insert

Tc3A

Tags

6xHis

Expression

Bacterial

Mutation

1-135 aa bipartite domain

Promoter

T7

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pNH030

Plasmid

#42149

Depositor

Colin Dolphin

Article

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Insert

mTFP1::linker::2xNLS

Expression

Worm

Available Since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only

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pEG608

Plasmid

#40194

Depositor

Geraldine Seydoux

Article

Griffin et al Cell. 2011 Sep 16;146(6):955-68.

Insert

Mex-5 (RNAi resistant) (mex-5 Nematode)

Tags

3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

Expression

Worm

Mutation

Exon 2 recoded to be RNAi-resistant

Promoter

4.4 kb mex-5 promoter fragment

Available Since

Sept. 19, 2012

Availability

Academic Institutions and Nonprofits only
pAWp9-4

Plasmid

#73855

Purpose

pFUGW-UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

Depositor

Timothy Lu

Article

Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

Insert

UBCp-RFP-CMVp-GFP-U6p-RFP-sg1-U6p-GFP-sg1

Use

Lentiviral

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only
mg172

Plasmid

#34652

Depositor

Miriam Goodman

Article

Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

Insert

slo-1(A1;B1;C0) (slo-1 Nematode)

Use

Xenopus oocyte expression

Tags

Xenopus beta-globulin 3' UTR and Xenopus bet…

Available Since

Feb. 2, 2012

Availability

Academic Institutions and Nonprofits only

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