We narrowed to 5,499 results for: cdna expression plasmid

1. pcDNA-HSF1Δ∼PRD

   Plasmid

   #71725

   Purpose

   plasmid expressing mic-his tag phosphorylation-deficient hHSF1

   Depositor

   Lea Sistonen

   Article

   Budzynski et al Mol Cell Biol. 2015 Jul;35(14):2530-40.

   Insert

   phosphorylation-deficient HSF1 (HSF1 Human)
   

   Use

   Tags

   mac 6xHIS

   Expression

   Mammalian

   Mutation

   15 phosphorylation sites in RD mutated to Alanine

   Promoter

   Available since

   May 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. FLAG-HA-DRE-1-pcDNA3.1-

   Plasmid

   #52513

   Purpose

   expresses C. elegans DRE-1 in mammalian cells with FLAG-HA tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Dre-1 (dre-1 Nematode)
   

   Use

   Tags

   FLAG-HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-CRISPR-Puro-sgRPL3-62

   Plasmid

   #208647

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312968-39312987), 62 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.62 (RPL3 Synthetic, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-CRISPR-Puro-sgRPL3-56

   Plasmid

   #208979

   Purpose

   pcDNA3.1 based plasmid for transient transfection of sgRNA-cas9. Containing sgRNA targeting human RPL3 (GRCh38.p14_Chr22:39312868-39312887), 56 is a number given by www.e-crisp.org.

   Depositor

   Quanfu Ma

   Insert

   sgRNA targeting human RPL3 (ENST00000216146) C-terminal, No.56 (RPL3 Synthetic, Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and U6 in tandem

   Available since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-ACE2-GFP

   Plasmid

   #154962

   Purpose

   pcDNA3.1-ACE2-GFP

   Depositor

   Utpal Pajvani

   Article

   Bartolome et al Sci Rep. 2021 May 7;11(1):9803. doi: 10.

   Insert

   ACE2-GFP (ACE2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-FLAG-Lin28A

   Plasmid

   #51371

   Depositor

   Narry Kim

   Article

   Heo et al Mol Cell. 2008 Oct 24;32(2):276-84. doi:

   Insert

   Lin28A (LIN28A Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-endo-Nb80

   Plasmid

   #205518

   Purpose

   Endosome-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb80
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-endo-Nb37

   Plasmid

   #205514

   Purpose

   Endosome-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   endo-Nb37
   

   Use

   Tags

   2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger) and mChe…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-nuc-Nb80

   Plasmid

   #205519

   Purpose

   Nuclear-targeted Nb80 nanobody that binds and stabilizes β2AR active conformation, blocking Gαs coupling.

   Depositor

   Jin Zhang

   Article

   Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

   Insert

   nuc-Nb80
   

   Use

   Tags

   Nuclear localization signal (NLS) and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-nuc-Nb37

    Plasmid

    #205515

    Purpose

    Nuclear-targeted Nb37 nanobody that binds and stabilizes the active form of Gαs G-protein subunit.

    Depositor

    Jin Zhang

    Article

    Kwon et al Nature. 2022 Nov;611(7934):173-179. doi:

    Insert

    nuc-Nb37
    

    Use

    Tags

    Nuclear localization signal (NLS) and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. miR-722 pCDNA

    Plasmid

    #97162

    Purpose

    luciferase assay

    Depositor

    Qing Deng

    Article

    Hsu et al Proc Natl Acad Sci U S A. 2019 Aug 26. p

    Insert

    miR-722 (mir722 Zebrafish)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 H2B-mIFP T2A Mpro (C145A)

    Plasmid

    #163080

    Purpose

    Expresses inactive Mpro mutant and a near-infrared fluorescent protein mIFP fused to H2B

    Depositor

    Xiaokun Shu

    Insert

    Inactive Mpro mutant C145A and H2B-mIFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1-FRB-Cre-HiBiT

    Plasmid

    #177912

    Purpose

    FRB-Cre-HiBiT tag

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Mol Pharm. 2022 May 20. doi: 10.1021/acs

    Insert

    FRB-Cre-HiBiT
    

    Use

    Tags

    HiBiT tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)-myc-OGA(GS-544)

    Plasmid

    #193995

    Purpose

    plasmid encoding myc-tagged OGA(GS-544)

    Depositor

    Christina Woo

    Article

    Ge et al ACS Chem Biol. 2023 Jan 4. doi: 10.1021/

    Insert

    myc-OGA(GS-544)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1-tvD-Cit-tevs-DHFR

    Plasmid

    #116054

    Purpose

    Reporter plasmid for measuring protease activity

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    tvD-Cit-tevs-DHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-CMV-SV40NLS-OgeuIscBE193A-3XHA

    Plasmid

    #222861

    Purpose

    This plasmid codes for the OgeuIscB nickase

    Depositor

    Vincent Dion

    Article

    Mangin et al bioRxiv 2023.10.24.563270

    Insert

    OgeuIscB Nickase
    

    Use

    CRISPR

    Tags

    3X HA and Nucleoplasmin NLS and ITR2

    Expression

    Mammalian

    Mutation

    E193A for nickase mutation

    Promoter

    CMV

    Available since

    Oct. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.3-NL63-D14

    Plasmid

    #172666

    Purpose

    encodes NL63 spike protein, for pseudovirus production

    Depositor

    David Nemazee

    Article

    Dacon et al Science. 2022 Jul 12:eabq3773. doi: 10.1

    Insert

    HCNV63gp2 (HCNV63gp2 human coronavirus NL63)
    

    Use

    Lentiviral and Retroviral

    Tags

    NA

    Expression

    Mammalian

    Mutation

    c-terminal deletion of 14aa

    Promoter

    CMV

    Available since

    Jan. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1_ spike_del19

    Plasmid

    #155297

    Purpose

    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles

    Depositor

    Raffaele De Francesco

    Article

    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2

    Insert

    SARS-Cov2 spike_deleted (S SARS-Cov2)
    

    Use

    Generation of sars-cov-2-pseudotyped lentiviral p…

    Tags

    Expression

    Mutation

    Deletion in C-term 19 aa of spike

    Promoter

    CMV

    Available since

    Aug. 5, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pcDNA3-ShhN-Ren

    Plasmid

    #37677

    Depositor

    Philip Beachy

    Article

    Ma et al Cell. 2002 Oct 4;111(1):63-75.

    Insert

    shh (Shh Mouse)
    

    Use

    Tags

    renilla luciferase

    Expression

    Mammalian

    Mutation

    soluble form (see comments)

    Promoter

    CMV

    Available since

    Nov. 12, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCDNA3.0_LynSNAP IRES EGFP

    Plasmid

    #136625

    Purpose

    Plasmid encoding outer inner membrane localization of SNAP-Tag and EGFP

    Depositor

    Kai Johnsson

    Article

    Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

    Insert

    Lyn11-SNAP-Tag (IRES EGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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