Addgene

We narrowed to 12,329 results for: BASE;

Showing: 1661 - 1680 of 12329 results

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pU6_gRNA_handle_U6t

Plasmid

#49016

Purpose

gRNA expression empty vector (mammalian cells)

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Type

Empty backbone

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-OTI-TCRB

Plasmid

#131036

Purpose

Expresses OT-I TCRB

Depositor

Jeff Hammerbacher

Article

Aksoy et al bioRxiv

Insert

TCRB (Tcrb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV/T7

Available since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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p667-UBC-TLK2-V5-TurboID_IDG-K

Plasmid

#135250

Purpose

Gateway destination clone of TLK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

TLK2-V5-TurboID (TLK2 Human)

Use

Lentiviral; Gateway destination

Tags

V5-TurboID

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pFKM1

Plasmid

#64950

Purpose

source of KmR FRT cassette

Depositor

Herbert Schweizer

Article

Choi et al Nat Methods. 2005 Jun;2(6):443-8.

Insert

FRT-KmR-FRT

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pKA-141

Plasmid

#79832

Purpose

Expresses RpBphP1-mCherry

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

Insert

BphP1-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMVd1

Available since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagBFP

Plasmid

#67168

Purpose

CRISPaint universal donor for TagBFP fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagBFP

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-NanoLuc

Plasmid

#67178

Purpose

CRISPaint universal donor for NanoLuc fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

NanoLuc

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-TagGFP2

Plasmid

#67166

Purpose

CRISPaint universal donor for TagGFP2 fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

TagGFP2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pGCS-C3m(3xFLAG)

Plasmid

#85726

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Jan. 9, 2017

Availability

Academic Institutions and Nonprofits only

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NPC1 His6 ECFP

Plasmid

#53522

Depositor

Matthew Scott

Article

Lopez et al J Neurosci. 2011 Mar 23;31(12):4367-78.

Insert

NPC1

Use

Tags

ECFP and His6

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
PaGFP-UtrCH

Plasmid

#26738

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

photoactivatable GFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGCS-N3(3xFLAG)

Plasmid

#85720

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

Feb. 16, 2017

Availability

Academic Institutions and Nonprofits only

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mRFP-UtrCH

Plasmid

#26739

Depositor

William Bement

Article

Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

Insert

Utrophin

Use

Reporter for f-actin

Tags

monomeric RFP

Expression

Mammalian

Mutation

Calponin homology domain of utrophin (aa# 1-261)

Promoter

Available since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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p667-UBC-PRKCQ-V5-miniTurbo_IDG-K

Plasmid

#170590

Purpose

Gateway destination clone of PRKCQ (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

PRKCQ-V5-miniTurbo (PRKCQ Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-YhjH

Plasmid

#79165

Purpose

Expresses YhjH under a T7 promoter

Depositor

Ming Hammond

Article

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

yhjH

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ZnGreen1

Plasmid

#169470

Purpose

cytosolic ZnGreen1 zinc sensor with 633 nM Kd

Depositor

Huiwang Ai

Article

Chen et al Anal Chem. 2016 Sep 20;88(18):9029-36. d

Insert

ZnGreen1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pSplit-NO-Dib1

Plasmid

#58786

Purpose

Express LucN-hDib1 fusion protein in mammalian cells

Depositor

Hiroyoshi Ariga

Article

Maita et al Anal Biochem. 2014 Feb 3. pii: S0003-269

Insert

hDIb1

Use

Tags

Flag and Luciferase (1-398aa)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2014

Availability

Academic Institutions and Nonprofits only
pGCS-C2m(3xHA)

Plasmid

#85725

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

3xHA

Expression

Mutation

Promoter

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_27F3_hFc

Plasmid

#199619

Purpose

Encodes DARPin-hFc 27F3 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc 27F3

Use

Tags

hFc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPaint-3xFlag

Plasmid

#67170

Purpose

CRISPaint universal donor for flag fusion tagging

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

3xFlag

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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