We narrowed to 7,176 results for: cas9 plasmid

1. pTL-Bag4-gRNA4

   Plasmid

   #196255

   Purpose

   Plasmid for cloning the 4th CRISPR-Cas9 guide RNA of 4 guide RNAs

   Depositor

   Bing Yang

   Article

   Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

   Insert

   CRISPR-Cas9 guide RNA scaffold and multiple cloning site
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119 promoter

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. M-ST1-VPR

   Plasmid

   #68498

   Purpose

   nuclease competent ST1-Cas9 fused to VPR

   Depositor

   George Church

   Article

   Kiani et al Nat Methods. 2015 Sep 7. doi: 10.1038/nm

   Insert

   ST-Cas9-VPR
   

   Use

   Tags

   VPR

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-dSa VP64 Hbb

   Plasmid

   #99693

   Purpose

   Expresses dSa Cas9 fused to gold standard VP64 activator domain and Sa sgRNA for Hbb promoter, vector allows for activation of mouse Hbb, can be packaged and delivered as AAV

   Depositor

   George Church

   Article

   Vora et al bioRxiv 2018. 298620

   Insert

   dCas9 and gRNA targeting Hbb (gRNA: GGGGTAAGGGGAGCAAGGTC) (Hbb Synthetic, Mouse)
   

   Use

   AAV and CRISPR

   Tags

   VP64

   Expression

   Mutation

   dead Cas9

   Promoter

   Available since

   May 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pDIV495

   Plasmid

   #177702

   Purpose

   Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

   Depositor

   Uffe Mortensen

   Article

   Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

   Inserts

   Cas9
   Nat
   sgRNA Targeting ADE2 locus in D.hansenii: AGCTAaGCAGATTAAtGCAT
   

   Use

   CRISPR

   Tags

   SV40

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   RNR2p (Debaryomyces hansenii ), SRC1p (Debaryomyc…

   Available since

   March 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDD104 (Peft-3::Cre)

   Plasmid

   #47551

   Purpose

   Cre recombinase expression plasmid for removal of floxed selectable markers in the C. elegans germline

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Nat Methods. 2013 Sep 1. doi: 10.1038/nm

   Insert

   Cre Recombinase
   

   Use

   Cre/Lox

   Tags

   Expression

   Worm

   Mutation

   Promoter

   eef-1A.1 (eft-3)

   Available since

   Sept. 9, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-SpRY-P2A-mScarlet (MNW002)

   Plasmid

   #174137

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R); C-terminal bi-partite NLS, 3x flag tag, P2A-mScarlet

   Depositor

   Benjamin Kleinstiver

   Insert

   human codon optimized SpRY with BPNLS-3xFLAG-P2A-mScarlet
   

   Use

   In vitro transcription; t7 promoter

   Tags

   BPNLS-3xFLAG-P2A-mScarlet

   Expression

   Mammalian

   Mutation

   SpG=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317…

   Promoter

   CMV and T7

   Available since

   Sept. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pUC57-Simple-gRNA backbone

   Plasmid

   #51306

   Purpose

   gRNA expression plasmid

   Depositor

   Yonglong Chen

   Article

   Guo et al Development. 2014 Jan 8.

   Type

   Empty backbone

   Use

   CRISPR; Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. AP582-2

   Plasmid

   #70049

   Purpose

   Expresses Ollas::mCherry::linker::H2B::V5 in bacteria

   Depositor

   Geraldine Seydoux

   Article

   Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

   Insert

   mCherry::linker::H2B
   

   Use

   Tags

   Ollas and V5

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKLV2-U6(gDmap1 v1)-PGK-Puro-BFP

   Plasmid

   #117140

   Purpose

   Express gRNA vs Dmap1

   Depositor

   Kosuke Yusa

   Insert

   gDmap1 v1 (Dmap1 Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Deleted BbsI site within WPRE, modified BFP

   Promoter

   human U6 promoter, mouse PGK promoter

   Available since

   April 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSc1-DD

    Plasmid

    #80439

    Purpose

    Expresses eGFP with ecDHFR along with an U6 promoter driven gRNA which cleaves the plasmid in-cell

    Depositor

    Ervin Welker

    Article

    Talas et al DNA Res. 2017 Jun 30. doi: 10.1093/dnare

    Inserts

    sp Cas9 gRNA
    eGFP
    

    Use

    CRISPR

    Tags

    E. coli dihydrofolate reductase

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    Aug. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pXPR_047

    Plasmid

    #107145

    Purpose

    measures Cas9 activity

    Depositor

    David Root

    Insert

    GFP + sgRNA for GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pORFE0001

    Plasmid

    #112070

    Purpose

    AtCas9 module CDS1

    Depositor

    Christian Chevalier

    Insert

    AtCAS9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Plant codon optimized Cas9 S. pyogenes

    Promoter

    Available since

    Aug. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. HCP9

    Plasmid

    #166111

    Purpose

    This plasmid encodes a Cas9 protein as well as a sgRNA that targets the C-terminus of Hta2

    Depositor

    Matthias Heinemann

    Insert

    Hta2-sg18 (HTA2 Budding Yeast)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. lentiCRISPR - EGFP sgRNA 1

    Plasmid

    #51760

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX458M-53BP1-DN1S

    Plasmid

    #131045

    Purpose

    Plasmid encoding Cas9-53BP1-DN1S fusion with BbsI restriction sites for insertion of guide RNA sequence.

    Depositor

    Punam Malik

    Article

    Jayavaradhan et al Nat Commun. 2019 Jun 28;10(1):2866. doi:

    Insert

    SpCas9-53BP1-DN1S (TP53BP1 Human)
    

    Use

    CRISPR

    Tags

    GFP and SpCas9

    Expression

    Mammalian

    Mutation

    Only expressing residues 1231-1644 of complete 53…

    Promoter

    CBH

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. lentiCRISPR - EGFP sgRNA 2

    Plasmid

    #51761

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCRISPR - EGFP sgRNA 4

    Plasmid

    #51763

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 4
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. lentiCRISPR - EGFP sgRNA 5

    Plasmid

    #51764

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 5
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiCRISPR - EGFP sgRNA 3

    Plasmid

    #51762

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 3
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS and U6

    Available since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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