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EF1a_GATA1_P2A_Hygro_Barcode

Plasmid

#120442

Purpose

Barcoded lentiviral vector to express GATA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

GATA1 (GATA1 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp13_GC3opt

Plasmid

#157714

Purpose

mammalian expression of untagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp13-HF_GC3opt (ORF1ab SARS-CoV-2)

Expression

Mammalian

Mutation

codon optimized; gene expression was optimized by…

Available Since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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Chicken ArcLight A173

Plasmid

#53615

Purpose

Genetically encoded voltage sensor ArcLight with improved kinetics

Depositor

Vincent Pieribone

Article

Han et al PLoS One. 2013 Nov 27;8(11):e81295. doi:

Insert

Chicken ArcLight-A173

Expression

Mammalian

Mutation

Gg-VSP contains an R153Q mutation and an amino ac…

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBW_0141

Plasmid

#102820

Purpose

pVec8 containing Sr45 gene

Depositor

Brande Wulff

Article

Md Hatta et al Mol Plant Microbe Interact. 2020 Nov;33(

Insert

wheat stem rust resistance gene Sr45 (NEWENTRY Synthetic)

Expression

Plant

Promoter

Sr33

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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YFVdelCMENS1-Cre

Plasmid

#179954

Purpose

pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Cre

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking C, M, E and NS1 proteins but with Cre

Use

Transneuronal tracing

Promoter

SP6

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-WWE-Linker-eGFP

Plasmid

#176072

Purpose

EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

WWE

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pDipA-TEV-His12

Plasmid

#137042

Purpose

expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Insert

AAC66790.1 (BB_0418 Borrelia burgdorferi B31)

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Mutation

wild type, full-length protein, contains signal p…

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-Hygro-EF1A-FHA-Linker-eGFP

Plasmid

#176065

Purpose

EGFP fused to the C-terminus of a FHA domain & a hygromycin resistance cassette

Depositor

Robert Sobol

Article

Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

Insert

FHA

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-Nsp10-HF

Plasmid

#157708

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp10 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp10-HF (ORF1ab SARS-CoV-2)

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Available Since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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MSCV-NSD3-short

Plasmid

#72552

Purpose

expresses 3*FLAG tagged human NSD3-short

Depositor

Christopher Vakoc

Article

Shen et al Mol Cell. 2015 Nov 24. pii: S1097-2765(1

Insert

NSD3-short (NSD3 Human)

Use

Retroviral

Tags

3*FLAG

Expression

Mammalian

Mutation

none

Promoter

LTR

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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