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We narrowed to 2,218 results for: dcas9

Showing: 1681 - 1700 of 2218 results

  1. IX907: pMVP (L3-L2) 3x epitope tag + WPRE

    Plasmid
    #121757
    Purpose
    pMVP L3-L2 entry plasmid, contains 3x HA epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x HA epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121757-plasmid-map-sequence-id-237237

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  2. JA001: pMVP (L3-L2) FLAG epitope tag + WPRE

    Plasmid
    #121758
    Purpose
    pMVP L3-L2 entry plasmid, contains FLAG epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    FLAG epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121758-plasmid-map-sequence-id-237239

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  3. IZ901: pMVP (L3-L2) OLLAS epitope tag + WPRE

    Plasmid
    #121759
    Purpose
    pMVP L3-L2 entry plasmid, contains OLLAS epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    OLLAS epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121759-plasmid-map-sequence-id-237241

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  4. IZ702: pMVP (L3-L2) myc epitope tag + WPRE

    Plasmid
    #121760
    Purpose
    pMVP L3-L2 entry plasmid, contains myc epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    myc epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121760-plasmid-map-sequence-id-237242

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  5. IZ801: pMVP (L3-L2) 6x His epitope tag + WPRE

    Plasmid
    #121761
    Purpose
    pMVP L3-L2 entry plasmid, contains 6x His epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    6x His epitope tag + WPRE
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121761-plasmid-map-sequence-id-237243

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  6. IH201: pMVP (L5-L4) 3x HA epitope tag

    Plasmid
    #121700
    Purpose
    pMVP L5-L4 entry plasmid, contains 3x HA epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x HA epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121700-plasmid-map-sequence-id-237048

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  7. KG703: pMVP (L5-L4) 6x His epitope tag

    Plasmid
    #121706
    Purpose
    pMVP L5-L4 entry plasmid, contains 6x His epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    6x His epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121706-plasmid-map-sequence-id-237120

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  8. IO801: pMVP (L5-L4) 3x FLAG epitope tag

    Plasmid
    #121702
    Purpose
    pMVP L5-L4 entry plasmid, contains 3x FLAG epitope tag for 4-component MultiSite Gateway Pro assembly. Allows N-term epitope tag fusion to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    3x FLAG epitope tag (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available Since
    March 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    121702-plasmid-map-sequence-id-239011

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  9. pORFE0003

    Plasmid
    #112072
    Purpose
    AtDeadCAS9 D10A H840A module CDS1
    Depositor
    Christian Chevalier
    Insert
    AtDeadCAS9 D10A H840A
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112072-plasmid-map-sequence-id-217628

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  10. pRAC10A

    Plasmid
    #193734
    Purpose
    Expresses deactivated-Cas9 (J23110) and tracrRNA, with BBR1 origin, ampR, Start-Stop assembly site for array insertion
    Depositor
    Martin Buck
    Insert
    dCas9
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193734-plasmid-map-sequence-id-381905

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  11. pORFE0014

    Plasmid
    #112076
    Purpose
    AtCAS9 nickase D10A module CDS1ns
    Depositor
    Christian Chevalier
    Insert
    AtCAS9 D10A without stop codon
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Plant codon optimized dCas9 D10A S. pyogenes
    Promoter
    Available Since
    Aug. 3, 2018
    Availability
    Academic Institutions and Nonprofits only
    112076-plasmid-map-sequence-id-217621

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  14. pETM6-G6-vioABE

    Plasmid
    #73438
    Purpose
    Prodeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    PG6-vioABE
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PG6 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73438-plasmid-map-sequence-id-135337

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  16. MSP712

    Plasmid
    #65768
    Purpose
    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA
    Depositor
    Keith Joung
    Article
    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature
    Insert
    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Bacterial
    Mutation
    D10A and H840A mutations in Cas9
    Promoter
    T7 (x2)
    Available Since
    June 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    65768-plasmid-map-sequence-id-112655

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  17. LLP773_pLenti-6xHRBKET-sgRNA

    Plasmid
    #211766
    Purpose
    Multiplexed gRNA plasmid targeting six different gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1), with mCherry selection
    Depositor
    Ryan Lister
    Article
    Swain et al Nucleic Acids Res. 2023 Nov 24:gkad1108.
    Insert
    gRNAs against six different human gene promoters (PACC1-2, EPCAM-2, B2M-4, KL-3, RBM3-1, HINT1-1)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    pCMV and U6
    Available Since
    Dec. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    211766-plasmid-map-sequence-id-417396

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  18. pETM6-4F2-mCherry

    Plasmid
    #73421
    Purpose
    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.
    Depositor
    Mattheos Koffas
    Article
    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw
    Insert
    Promoter 4F2 (orthogonal T7-lac variant)
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P4F2 (orthogonal T7-lac variant)
    Available Since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    73421-plasmid-map-sequence-id-135321

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  19. JE301: pMVP (L5-L4) Tir1-P2A-AID

    Plasmid
    #121722
    Purpose
    pMVP L5-L4 entry plasmid, contains Tir1-P2A-AID for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Tir1 linked by P2A to AID domain fused to gene of interest.
    Depositor
    Christopher Newgard
    Article
    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526
    Insert
    osTir1-P2A-AID (open)
    Use
    Synthetic Biology; Pmvp gateway entry plasmid
    Tags
    9x myc (C-term on osTIR1)
    Expression
    Mutation
    Promoter
    Available Since
    June 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    121722-plasmid-map-sequence-id-237078

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Showing: 1681 - 1700 of 2218 results