We narrowed to 6,489 results for: puc

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pAAV-dSa-VP64-p65(100-261)-RTA(125-190)

Plasmid

#99679

Purpose

Expresses dSa Cas9 fused to optimized combination of truncated activation domains

Depositor

George Church

Article

Vora et al bioRxiv 2018. 298620

Insert

dCas9

Use

AAV

Tags

VP64-p65(101-261)-RTA(125-190)

Mutation

dead Cas9

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pBBK04 pCAS-Tyr-[gRNA: BsaI GFP dropout] (SplitHygR,AmpR)

Plasmid

#178988

Purpose

Sp.Cas9 and gRNA yeast expression vector for Golden Gate pre-cloning of gRNA. Selection = SplitHygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pX330 EGFR-sgRNA

Plasmid

#188633

Purpose

A human codon-optimized SpCas9 and chimeric guide RNA targeting C-terminus of human EGFR

Depositor

Jared Toettcher

Article

Farahani et al eLife. 2023 May 22;12:e82863. doi: 10.75

Insert

humanized S. pyogenes Cas9

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Promoter

CBh

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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Bxb1-GA_CAG-TurboGFP

Plasmid

#229781

Purpose

Bxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP

Depositor

Richard Davis

Article

Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

TurboGFP

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Promoter

CAG

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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pBBK95 pCAS-Tyr-[gRNA: 2xBsaI+NotI cut sites] (HygR)

Plasmid

#179079

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Hygromycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pXFokI-dCas9

Plasmid

#60901

Purpose

Dual Expression Vector for FokI-dCas9 and gRNA

Depositor

Bruce Conklin

Article

Miyaoka et al Sci Rep. 2016 Mar 31;6:23549. doi: 10.10

Type

Empty backbone

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Promoter

CBh and U6

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only

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pBBK01 pCAS-Tyr-[gRNA: BsaI GFP dropout] (KanR, AmpR)

Plasmid

#178985

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Kanamycin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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EZ-L439

Plasmid

#169146

Purpose

Encodes OptoINVRT7 circuit

Depositor

Jose Avalos

Article

Zhao et al ACS Synth Biol. 2020 Dec 18;9(12):3254-3

Insert

VP16-EL222

Tags

SV40 NLS

Expression

Yeast

Available Since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBBK05 pCAS-Tyr-[gRNA: BsaI GFP dropout] (NatR,AmpR)

Plasmid

#178989

Purpose

Sp.Cas9 and gRNA yeast expression vector for HDR-based gRNA introduction. Selection = Nourseothricin resistance

Depositor

Vincent Martin

Article

Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

Insert

S. pyogenes Cas9

Use

CRISPR and Synthetic Biology

Expression

Yeast

Available Since

May 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-Cas9-tdTomato

Plasmid

#141013

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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KG#65

Plasmid

#110933

Purpose

Expresses proteins in cholinergic neurons in the ventral nerve cord of C. elegans

Depositor

Kenneth Miller

Article

Charlie et al Genetics. 2006 Feb;172(2):943-61. doi: 1

Inserts

unc-17 beta promoter
unc-54 3' control region

Expression

Bacterial

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg1

Plasmid

#87189

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 1

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #1 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg2

Plasmid

#87190

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 2

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #2 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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KG#707

Plasmid

#110911

Purpose

Expresses the C. elegans sup-1(e995) minigene in ventral nerve cord cholinergic motor neurons

Depositor

Kenneth Miller

Article

Harterink et al J Cell Sci. 2018 Oct 22;131(20). pii: jc

Insert

sup-1 minigene

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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px330-MRPsg4

Plasmid

#87192

Purpose

disruption of human RMRP gene via CRISPR-Cas9, guide 4

Depositor

Thomas Cech

Article

Goldfarb et al Genes Dev. 2017 Jan 1;31(1):59-71. doi:

Insert

human MRP RNA CRISPR guide #4 (RMRP Human)

Use

CRISPR

Expression

Mammalian

Promoter

human U6

Available Since

March 24, 2017

Availability

Academic Institutions and Nonprofits only

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KG#230

Plasmid

#110925

Purpose

Vector for expressing proteins in a subset of 9 DA/ DB cholingergic motor neurons in the C. elegans ventral nerve cord

Depositor

Kenneth Miller

Article

Edwards et al J Cell Biol. 2009 Sep 21;186(6):881-95.

Inserts

unc-129 promoter
unc-54 3' control region

Expression

Bacterial

Available Since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

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pNB-Rep

Plasmid

#238996

Purpose

Node B carrying the sfGPF reporter that can be used for the assembly of pEND-11Y, pEND-Y11, pEND-Z11 or pEND-ZY

Depositor

Javier Santos-Moreno

Article

Nevot et al ACS Synth Biol. 2025 Aug 15;14(8):2878-2

Insert

sfGFP-MarAn10 downstream of a strong constitutive promoter and a binding site for sgRNA-1

Use

Synthetic Biology

Expression

Bacterial

Mutation

WT

Available Since

Dec. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pBBM

Plasmid

#243675

Purpose

Expresses a maize optimized CDS of BABY BOOM in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

Maize optimized CDS of BABY BOOM

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pWUS2

Plasmid

#243676

Purpose

Expresses a maize optimized CDS of WUSCHEL2 in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

Maize optimized CDS of WUSHCEL2

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pStayGold

Plasmid

#243674

Purpose

Expresses StayGold fluorescent protein in plants using the Cestrum Yellow Leaf Curling Virus (CmYLCV) 9.11 promoter and an Arabidopsis HSP terminator

Depositor

Patrick Krysan

Article

De La Mora et al Plant Cell Rep. 2025 Oct 19;44(11):243.

Insert

StayGold fluorescent protein

Expression

Plant

Available Since

Nov. 4, 2025

Availability

Academic Institutions and Nonprofits only

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