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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,008 results for: rigi

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Showing: 1681 - 1700 of 2008 results

  1. MSRP2-bio

    Plasmid
    #47737
    Purpose
    Expresses enzymatically monobiotinylated full-length MSRP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSRP2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47737-plasmid-map-sequence-id-68873

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  2. MSRP3-bio

    Plasmid
    #47738
    Purpose
    Expresses enzymatically monobiotinylated full-length MSRP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSRP3
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47738-plasmid-map-sequence-id-68874

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  4. GTL-ir

    Plasmid
    #81102
    Purpose
    Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    codon optimized second iRFP to avoid internal rec…
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81102-plasmid-map-sequence-id-154289

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  6. pLJSRSF7-T7-6xHis-SUMO-MRT-CBD

    Plasmid
    #253350
    Purpose
    For the expression of group II intron encoded reverse transcriptase MarathonRT with N-terminal SUMO-tag and C-terminal CBD-tag in E. coli.
    Depositor
    Peter Sarin
    Article
    Pedor et al Bio-protocol 2026, 16(11): e5703. DOI: 1
    Insert
    6xHis-SUMO-MarathonRT with added C-terminal CBD-tag originating from the backbone.
    Tags
    6xHis-SUMO and CBD
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 11, 2026
    Availability
    Academic Institutions and Nonprofits only
    253350-plasmid-map-sequence-id-507308

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  7. pYSD741

    Plasmid
    #253052
    Purpose
    Encodes MFapp8 Sig Pep, Hydrophobin1 (HFB1), MYC-TEV-649-stalk surface display anchor in a yeast expression vector with pTEF2 promoter, tENO2 terminator, HIS3 selectable marker and CEN/ARS origin
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    Hydrophobin1 (HFB1)
    Expression
    Yeast
    Available Since
    May 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    253052-plasmid-map-sequence-id-508946

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  11. pYSD288

    Plasmid
    #253023
    Purpose
    Encodes the S. cerevisiae Aga1 protein (Part3) with pGAL1 inducible promoter, tENO2 terminator, KanamycinR yeast selectable marker and CEN/ARS yeast origin
    Depositor
    Paul Young
    Article
    Juric et al ACS Synth Biol. 2026 Apr 23. doi: 10.102
    Insert
    AGA1
    Expression
    Yeast
    Available Since
    April 22, 2026
    Availability
    Academic Institutions and Nonprofits only
    253023-plasmid-map-sequence-id-507339

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  12. pM3_PLux*R1CI*_PLTetO1R1CI*_PLCR1E2Cr_PJ23102R1LuxR

    Plasmid
    #250890
    Purpose
    LuxR expressed under BBa_J23102; E2-Crimson under PLC promoter; mutated cI* under PLTetO1; and cI* under Plux*; part of BNeuAS5; pUC origin of replication
    Depositor
    Sangram Bagh
    Article
    Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534
    Inserts
    E2-Crimson
    LuxR
    Mutated cI
    Mutated cI
    Use
    Synthetic Biology
    Mutation
    Frame shifted cI protein and Frame shifted cI pro…
    Promoter
    BBa_J23102, PLC, PLTetO1, and Plux
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    250890-plasmid-map-sequence-id-502190

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  13. pK1_PLLacO1R3CIw_PLux*R1cI*

    Plasmid
    #250891
    Purpose
    Frame shifted cI protein expressed under PLux* promoter; wild type cI expressed under PLLacO1 promoter; component of BNeuAS6; p15A origin of replication
    Depositor
    Sangram Bagh
    Article
    Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534
    Inserts
    Mutated cI
    Wild type cI
    Use
    Synthetic Biology
    Mutation
    cI* = Frame shifted cI protein; PLux* = Point mut…
    Promoter
    PLLacO1 and PLux*
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    250891-plasmid-map-sequence-id-500505

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  14. pBIRC5-Split PS Intein-N tTA

    Plasmid
    #242033
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.
    Depositor
    Vladislav Verkhusha
    Article
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the BIRC5 prom…
    Promoter
    hTERT
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242033-plasmid-map-sequence-id-483131

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  15. phTERT-Split PS Intein-N tTA

    Plasmid
    #242034
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.
    Depositor
    Vladislav Verkhusha
    Article
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the hTERT prom…
    Promoter
    hTERT
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242034-plasmid-map-sequence-id-481812

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  16. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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Showing: 1681 - 1700 of 2008 results