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We narrowed to 1,966 results for: rigi

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Showing: 1681 - 1700 of 1966 results

  1. MSRP2-bio

    Plasmid
    #47737
    Purpose
    Expresses enzymatically monobiotinylated full-length MSRP2 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSRP2
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47737-plasmid-map-sequence-id-68873

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  2. MSRP3-bio

    Plasmid
    #47738
    Purpose
    Expresses enzymatically monobiotinylated full-length MSRP3 with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.
    Depositor
    Gavin Wright
    Article
    Crosnier et al Mol Cell Proteomics. 2013 Sep 16.
    Insert
    Codon-optimised MSRP3
    Tags
    enzymatic biotinylation sequence and rat Cd4 d3+4
    Expression
    Mammalian
    Mutation
    Exogenous signal peptide of mouse origin, changed…
    Promoter
    CMV
    Available Since
    Feb. 4, 2014
    Availability
    Academic Institutions and Nonprofits only
    47738-plasmid-map-sequence-id-68874

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  4. GTL-ir

    Plasmid
    #81102
    Purpose
    Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)
    Depositor
    Serge Pelet
    Article
    Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231
    Insert
    tdiRFP
    Use
    Cre/Lox
    Tags
    tdiRFP
    Expression
    Yeast
    Mutation
    codon optimized second iRFP to avoid internal rec…
    Available Since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    81102-plasmid-map-sequence-id-154289

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  6. pM3_PLux*R1CI*_PLTetO1R1CI*_PLCR1E2Cr_PJ23102R1LuxR

    Plasmid
    #250890
    Purpose
    LuxR expressed under BBa_J23102; E2-Crimson under PLC promoter; mutated cI* under PLTetO1; and cI* under Plux*; part of BNeuAS5; pUC origin of replication
    Depositor
    Sangram Bagh
    Article
    Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534
    Inserts
    E2-Crimson
    LuxR
    Mutated cI
    Mutated cI
    Use
    Synthetic Biology
    Mutation
    Frame shifted cI protein and Frame shifted cI pro…
    Promoter
    BBa_J23102, PLC, PLTetO1, and Plux
    Available Since
    March 23, 2026
    Availability
    Academic Institutions and Nonprofits only
    250890-plasmid-map-sequence-id-502190

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  7. pK1_PLLacO1R3CIw_PLux*R1cI*

    Plasmid
    #250891
    Purpose
    Frame shifted cI protein expressed under PLux* promoter; wild type cI expressed under PLLacO1 promoter; component of BNeuAS6; p15A origin of replication
    Depositor
    Sangram Bagh
    Article
    Bonnerjee et al Nat Chem Biol. 2024 Nov;20(11):1524-1534
    Inserts
    Mutated cI
    Wild type cI
    Use
    Synthetic Biology
    Mutation
    cI* = Frame shifted cI protein; PLux* = Point mut…
    Promoter
    PLLacO1 and PLux*
    Available Since
    Feb. 19, 2026
    Availability
    Academic Institutions and Nonprofits only
    250891-plasmid-map-sequence-id-500505

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  8. pREC1010-opEas1

    Plasmid
    #240701
    Purpose
    RSF1010 origin of replication plasmid containing Eas1 recombitron with extended a1 a2 regions in the ncRNA targeting lacZ locus in Klebsiella pneumoniae ATCC 10031 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eas1 RT, Eas1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    240701-plasmid-map-sequence-id-489548

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  9. pBIRC5-Split PS Intein-N tTA

    Plasmid
    #242033
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the BIRC5 promoter.
    Depositor
    Vladislav Verkhusha
    Article
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the BIRC5 prom…
    Promoter
    hTERT
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242033-plasmid-map-sequence-id-483131

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  10. phTERT-Split PS Intein-N tTA

    Plasmid
    #242034
    Purpose
    N-terminal segment of the blue-light-controlled split PS Intein tTA gene expression system, co-expressing mTagBFP2 under the hTERT promoter.
    Depositor
    Vladislav Verkhusha
    Article
    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:
    Insert
    Split PS Intein-N tTA
    Tags
    mTagBFP2
    Expression
    Mammalian
    Mutation
    The CMV promoter was replaced with the hTERT prom…
    Promoter
    hTERT
    Available Since
    Sept. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    242034-plasmid-map-sequence-id-481812

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  11. pREC1010C-Eco1

    Plasmid
    #240690
    Purpose
    RSF1010 origin of replication plasmid containing Eco1 recombitron with a SapI flanked stuffer in the ncRNA expressed by J23115 constitutive promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Eco1 RT, Eco1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Sept. 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    240690-plasmid-map-sequence-id-483599

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  12. pCR197

    Plasmid
    #240643
    Purpose
    Expression of the tri-cistronic construct NES-AmNeonGreen-P2A-mTurquoise2-NLS-T2A-mScarlet-I-PTS1 in Exaiptasia diaphana; In vitro transcription of the same construct via SP6 polymerase
    Depositor
    John Pringle
    Inserts
    AIPGENE865 promoter
    SP6 promoter
    AmNeonGreen
    mTurquoise2
    mScarlet-I
    Use
    Gene expression and genomic integration in fish
    Tags
    NES (from Exaiptasia diaphana MEK2), NLS (from SV…
    Mutation
    Codon-optimized for Exaiptasia diaphana, amino ac…
    Available Since
    Aug. 15, 2025
    Availability
    Academic Institutions and Nonprofits only
    240643-plasmid-map-sequence-id-482910

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  13. pREC1010-opEbu1

    Plasmid
    #240702
    Purpose
    RSF1010 origin of replication plasmid containing Ebu1 recombitron with extended a1 a2 regions with a donor in the ncRNA targeting lacZ locus in Citrobacter freundii ATCC 8090 expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Ebu1 RT, Ebu1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    Extended a1 a2 regions
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240702-plasmid-map-sequence-id-482374

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  14. pREC1010-Abe1

    Plasmid
    #240683
    Purpose
    RSF1010 origin of replication plasmid containing Abe1 recombitron with a SapI flanked stuffer in the ncRNA expressed by Pm promoter
    Depositor
    Seth Shipman
    Article
    González-Delgado et al bioRxiv 2025.06.16.660010
    Insert
    Abe1 RT, Abe1 ncRNA, CspRecT and EcSSB
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Aug. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    240683-plasmid-map-sequence-id-482346

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  19. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

    Plasmid
    #201533
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB2
    Use
    Synthetic Biology
    Mutation
    rhaR L128+1bp (frameshift), ilvA G455C
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201533-plasmid-map-sequence-id-398133

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  20. pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

    Plasmid
    #201535
    Purpose
    Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)
    Depositor
    Dan Park
    Article
    Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d
    Insert
    ilvA-relE_RBS3_CB4
    Use
    Synthetic Biology
    Mutation
    rhaS ΔM1-H5, PrhaSR Δ179bp
    Promoter
    PrhaBAD (rhamnose)
    Available Since
    June 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    201535-plasmid-map-sequence-id-398128

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Showing: 1681 - 1700 of 1966 results