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pICSL50011

Plasmid

#50317

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, EOS photoactivatable fluorescent protein (L. hemprichii)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL80001

Plasmid

#50326

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, luciferase (P. pyralis)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAGT707

Plasmid

#51835

Purpose

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

Tomato Mosaic virus 5'UTR+ CCAT

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

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pGL2_136

Plasmid

#199083

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_137

Plasmid

#199084

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Xt-VSP

Plasmid

#51887

Purpose

expression of Xt-VSP in Xenopus oocytes or mammalian cells

Depositor

Laurinda Jaffe

Article

Ratzan et al J Cell Physiol. 2011 Nov;226(11):2740-6.

Insert

VSP

Use

Xenopus oocyte expression

Expression

Mammalian

Mutation

see comments*

Promoter

CMV

Available Since

April 3, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE Rad

Plasmid

#11213

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1996 Jan 12. 271(2):768-73.

Insert

Rad (RRAD Human)

Use

Retroviral

Expression

Mammalian

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pIVEX-anchor

Plasmid

#46844

Purpose

Template for the amplification of spiking anchors in BeSD

Depositor

Florian Hollfelder

Article

Diamante et al Protein Eng Des Sel. 2013 Oct;26(10):713

Type

Empty backbone

Use

In vitro expression

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pHES833

Plasmid

#90409

Purpose

Expresses YFP under control of GAL1 promoter

Depositor

Hana El-Samad

Article

Aranda-Diaz et al ACS Synth Biol. 2016 Dec 27. doi: 10.102

Insert

pZ-YFP

Use

Synthetic Biology

Expression

Yeast

Available Since

Nov. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pICSL30006

Plasmid

#50303

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS no stop codon, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP16

Plasmid

#80670

Purpose

Encodes the synthetic coiled coil peptide SYNZIP16

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP16

Use

Gateway Cloning

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pICH44199

Plasmid

#50286

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Potato Virus X)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP12

Plasmid

#80667

Purpose

Encodes the synthetic coiled coil peptide SYNZIP12

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP12

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP13

Plasmid

#80668

Purpose

Encodes the synthetic coiled coil peptide SYNZIP13

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP13

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP14

Plasmid

#80669

Purpose

Encodes the synthetic coiled coil peptide SYNZIP14

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP14

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-SYNZIP10

Plasmid

#80665

Purpose

Encodes the synthetic coiled coil peptide SYNZIP10

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP10

Use

Gateway Cloning

Available Since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.CMVenh.gp91.GP91

Plasmid

#30478

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/GP91

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL.gp91.eGFP

Plasmid

#30465

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

gp91/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pMODloxZeo3DAmp

Plasmid

#27182

Depositor

Yoh Wada

Article

Aoyama et al Nucleic Acids Res. 2005 . 33(5):e52.

Insert

lox-EM7p-Zeo-lox cassette flanked by Tn5 outer element

Use

In vitro transposion

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pEPYC1CB0199

Plasmid

#154655

Purpose

MoClo Level 1 luciferase reporter

Depositor

Nicola Patron

Article

Cai et al Nucleic Acids Res. 2020 Aug 28. pii: 589

Insert

MAS Firefly luciferase expression cassette [MAS::TMV::LucF::Flag::ocsT]

Use

Synthetic Biology

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL80014

Plasmid

#50320

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, YFP variant of GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

March 7, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41373

Plasmid

#50252

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (1.3 kb), 35s (c)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pICSL50014

Plasmid

#50313

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

C terminal HBP tag (HA-PreScission-Biotin)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-SYNZIP19

Plasmid

#80673

Purpose

Encodes the synthetic coiled coil peptide SYNZIP19

Depositor

Amy Keating

Article

Thompson et al ACS Synth Biol. 2012 Apr 20;1(4):118-29.

Insert

SYNZIP19

Use

Gateway Cloning

Available Since

May 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pICH41531

Plasmid

#50321

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, GFP (A. victoria)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_230

Plasmid

#251941

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

Dummy ORI plus NGS barcode 23, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_313

Plasmid

#251951

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pIJ101 ORI plus NGS barcode 15, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_289

Plasmid

#251875

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pBP1 ORI plus NGS barcode 11, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

June 1, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_277

Plasmid

#251917

Purpose

Level 2 plasmid, barcoded ORI with kanamycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

RSF1010 ORI plus NGS barcode 14, kanamycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_332

Plasmid

#251860

Purpose

Level 2 plasmid, barcoded ORI with chloramphenicol resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pNG2 ORI plus NGS barcode 22, chloramphenicol resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_360

Plasmid

#251940

Purpose

Level 2 plasmid, barcoded ORI with spectinomycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

RSF1010 ORI plus NGS barcode 14, spectinomycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_304

Plasmid

#251942

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

p15A ORI plus NGS barcode 24, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 11, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_316

Plasmid

#251954

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pMB1 ORI plus NGS barcode 29, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_322

Plasmid

#251960

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pUB110 ORI plus NGS barcode 2, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_324

Plasmid

#251962

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

RK2 ORI plus NGS barcode 12, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_310

Plasmid

#251948

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pBP1 ORI plus NGS barcode 11, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 8, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_312

Plasmid

#251950

Purpose

Level 2 plasmid, barcoded ORI with tetracycline resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pCD6 ORI plus NGS barcode 27, tetracycline resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_293

Plasmid

#251879

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pIM13 ORI plus NGS barcode 28, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_249

Plasmid

#251869

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pBC1 ORI plus NGS barcode 25, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_248

Plasmid

#251868

Purpose

Level 2 plasmid, barcoded ORI with erythromycin resistance cassette and mScarlet-I reporter

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2024.02.23.581749

Insert

pSa ORI plus NGS barcode 16, erythromycin resistance cassette, mScarlet-I cassette, oriT

Use

Synthetic Biology

Available Since

May 7, 2026

Availability

Academic Institutions and Nonprofits only

Enlarge

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