We narrowed to 6,888 results for: GFP expression plasmids

1. AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

   Plasmid

   #208834

   Purpose

   Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

   Depositor

   Ben Emery

   Article

   Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

   Insert

   mCherry
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   hSyn1

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEGFP-C1-FLAG

   Plasmid

   #46956

   Purpose

   Plasmid for cloning and expression of GFP-FLAG tagged proteins (N-terminal tag). Confers resistance to G418.

   Depositor

   Steve Jackson

   Article

   Britton et al J Cell Biol. 2013 Jul 29.

   Insert

   FLAG tag
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. 2xMARS-nGFP

   Plasmid

   #205235

   Purpose

   Expresses two repeats of PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
   

   Use

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. MARS-nGFP

   Plasmid

   #205234

   Purpose

   Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody in mammalian cells

   Depositor

   Jeremy Baskin

   Insert

   PLEKHA5 aa 143-271 (K163A and R164A) fused to a HA-tagged GFP nanobody (PLEKHA5 Human)
   

   Use

   Tags

   HA, Nuclear Export Sequence, mScarlet-i, and nGFP…

   Expression

   Mammalian

   Mutation

   amino acids 143-271 of PLEKHA5 (GenBank reference…

   Promoter

   CMV

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pHBS1202 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 4xΦ]

   Plasmid

   #107845

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined in four clusters in spl…

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHBS1203 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 2xΦ]

   Plasmid

   #107844

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined in two clusters in spli…

   Promoter

   Available since

   April 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHBS1201 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 6xΦ]

   Plasmid

   #107846

   Purpose

   To test the effect of sequence on TDP43 splicing activity

   Depositor

   Rajat Rohatgi

   Insert

   TARDBP mutant (TARDBP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   All hydrophobics combined in six clusters in spli…

   Promoter

   Available since

   April 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEGFP-C1-alpha1-chimaerin

   Plasmid

   #59317

   Purpose

   Expression plasmid for GFP-tagged alpha1-chimaerin (GFP at N-terminus of alpha1-chimaerin)

   Depositor

   Peter Scheiffele

   Article

   Beg et al Neuron. 2007 Sep 6;55(5):768-78.

   Insert

   alpha1-chimaerin
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pBiex-1 GFPNanobody_SNAP

   Plasmid

   #82711

   Purpose

   Expresses a GFP binding nanobody linked to a SNAP tag in bacteria and Sf9 cells.

   Depositor

   Sivaraj Sivaramakrishnan

   Article

   Sommese et al Protein Sci. 2016 Aug 18. doi: 10.1002/p

   Insert

   GFP Nanobody
   

   Use

   Tags

   6xHis Purification tag, FLAG purification tag, an…

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   T7 Promoter

   Available since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSADdeltaG-GFP-mCherry

    Plasmid

    #32643

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Inserts

    GFP
    mCherry
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 26, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pMA-MsgRNA-EGFP

    Plasmid

    #80794

    Purpose

    For insertion of gRNA array containing 11-30 gRNA modules

    Depositor

    Yonglun Luo

    Article

    Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSADdeltaG-GFP-rtTA

    Plasmid

    #32648

    Depositor

    Edward Callaway

    Article

    Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

    Inserts

    GFP
    rtTA
    

    Use

    Rabies virus

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 3, 2011

    Availability

    Academic Institutions and Nonprofits only

14. 114_UBI_iCre_crystGFP

    Plasmid

    #171797

    Purpose

    Tol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)

    Depositor

    Jean Giacomotto

    Article

    Tromp et al Front Physiol. 2023 Aug 2;14:1221310. do

    Insert

    optimised iCre under the control of the Ubiquitin promoter
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Ubiquitin

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PAC_E83_LOV_eGFP

    Plasmid

    #231574

    Purpose

    This plasmid mediates puromycin resistance in the dark, resulting in puro mediated cell death upon illumination with blue light

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    PAC-P2A-GFP carrying an insertion of AsLOV2 behind E83
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJJF144_SECGFP_sg2

    Plasmid

    #163865

    Purpose

    sgRNA targeting GFP in C. elegans. Backbone: pJJR50.

    Depositor

    Nikolaus Rajewsky

    Article

    Froehlich et al Cell Rep. 2021 Apr 13;35(2):108988. doi:

    Insert

    sgRNA targeting GFP
    

    Use

    CRISPR

    Tags

    Expression

    Worm

    Mutation

    Promoter

    R07E5.16 (U6)

    Available since

    April 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-hSyn-sbGLuc-CheRiff-EGFP

    Plasmid

    #114108

    Purpose

    fusion protein of Gaussia luciferase variant slow burn, synthetic channelrhodopsin CheRiff, and enhanced green fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Ikefuama et al Int J Mol Sci. 2022 Oct 27;23(21):12994.

    Insert

    Gaussia luciferase, slow burn; CheRiff synthetic ChR; EGFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. FUGW U6 gL1HSg1dCas9-KRAB-T2a-GFP

    Plasmid

    #234882

    Purpose

    L1HS-silencing plasmid (CRISPRi gRNA1)

    Depositor

    Johan Jakobsson

    Article

    Adami et al bioRxiv 2025.01.17.633315

    Insert

    L1HS-silencing plasmid (CRISPRi gRNA1)
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Lenti-(BB)-EF1a-KRAB-dCas9-P2A-BlastR EGFP-guide1

    Plasmid

    #118158

    Purpose

    CRISPRi negative control. Catalytically inactive Cas9 from S. pyogenes with 2A-BlastR under the EF1a core promoter, and sgRNA1 against the EGFP CDS

    Depositor

    Jorge Ferrer

    Insert

    KRAB-dCas9-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys

    Plasmid

    #90473

    Purpose

    Elastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal Cysteine

    Depositor

    Hermann Gaub

    Insert

    TagGFP2-TEV-GGG-ELP(120nm)-Cys
    

    Use

    Tags

    Cysteine and Sortase-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge