We narrowed to 2,364 results for: PUC19

1. pML2(−)BR

   Plasmid

   #108340

   Purpose

   MIDAS Level-2 shuttle vector for assembling transcription units (TUs) with reverse orientation and (−) polarity. TUs assembled in this vector are used for BsmBI-mediated Level-3 multigene assembly.

   Depositor

   Andrew Muscroft-Taylor

   Article

   van Dolleweerd et al ACS Synth Biol. 2018 Apr 20;7(4):1018-10

   Type

   Empty backbone

   Use

   Synthetic Biology

   Available Since

   June 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPN218

   Plasmid

   #91677

   Purpose

   Express sgRNA targeting human TLE3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   TLE3 (TLE3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPN219

   Plasmid

   #91678

   Purpose

   Express sgRNA targeting human TLE3

   Depositor

   Lindy Barrett

   Article

   Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

   Insert

   TLE3 (TLE3 Human)
   

   Use

   CRISPR

   Promoter

   U6

   Available Since

   Oct. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTB105

   Plasmid

   #236778

   Purpose

   Backbone for cloning sgRNAs to be used with CRISPR Cas9 cytosine base editor. Expressed from the 18S rRNA SSU locus in Leishmania. Contains puromycin selection marker.

   Depositor

   Tom Beneke

   Article

   Herrmann May et al eLife. 2025 Feb 24;13:RP97437. doi: 10.7

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Oct. 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHS1376

   Plasmid

   #239829

   Purpose

   WT OrufIscB omegaRNA mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   OrufIscB omegaRNA scaffold
   

   Expression

   Mammalian

   Promoter

   human U6

   Available Since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pILFPZ1A9

   Plasmid

   #223835

   Purpose

   Expressing a cytosolic pH sensor (mTagBFP2-mNeonGreen) in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

   Insert

   pTEF1>mTagBFP>mNeonGreen>
   

   Expression

   Yeast

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pILFPmTagBFP2FrFAST

   Plasmid

   #223839

   Purpose

   Expressing mTagBFP2-FrFAST under the control of the TEF1 promtoer in Saccharomyces cerevisiae

   Depositor

   Claudia Vickers

   Article

   Evans et al ACS Synth Biol. 2025 Sep 19;14(9):3804-3

   Insert

   pTEF1>mTagBFP2>FrFAST
   

   Expression

   Yeast

   Promoter

   TEF1

   Available Since

   June 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYI277

   Plasmid

   #228285

   Purpose

   Constitutive EGFP expression

   Depositor

   Jun Ishii

   Article

   Tominaga et al Nat Commun. 2024 Dec 19;15(1):10653. doi

   Insert

   EGFP
   

   Use

   Synthetic Biology

   Expression

   Yeast

   Promoter

   KpGAPDH promoter

   Available Since

   March 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM097

   Plasmid

   #216819

   Purpose

   Aspergillus nidulans codon-adjusted mStayGold fluorescent protein, includes linker for N-terminal or internal tagging.

   Depositor

   Ken Sawin

   Article

   Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

   Insert

   mStayGold
   

   Tags

   FLAG-(SGGS)x2-XTEN16-(GGGGS)x3 and c4-(GGGGS)x2-X…

   Expression

   Bacterial

   Mutation

   Aspergillus nidulans codon-adjusted

   Available Since

   Sept. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pGGA-pTRE-B

    Plasmid

    #218894

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    TRE
    

    Use

    Synthetic Biology

    Mutation

    580G>C

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGGF-WPRE-G

    Plasmid

    #218901

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    WPRE
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGD_MUTT2A_E

    Plasmid

    #218899

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    MUTT2A
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGGD-T2A-E

    Plasmid

    #218898

    Purpose

    golden gate cloning

    Depositor

    Sven Eyckerman

    Article

    Delhaye et al Cell Rep Methods. 2024 Jul 15;4(7):10081

    Insert

    T2A
    

    Use

    Synthetic Biology

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p931-SWITCH-OVER insert: EF1a-Blasti

    Plasmid

    #217890

    Purpose

    insert vector for Switch-OVER: Single loxP-STOP-EF1a-Blasti-mU6

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. p223-Pulse-Switch

    Plasmid

    #217889

    Purpose

    Lentiviral Pulse-Switch vector for sgRNA expression, Cas9 and sgRNA expression are mutual exclusive; blastiR

    Depositor

    Ulrich Elling

    Article

    Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

    Type

    Empty backbone

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. NK811

    Plasmid

    #196408

    Purpose

    Nanoluc luciferase under S. rosetta SPAG6 promoter with mutated RFX binding site with internal actin-firefly control

    Depositor

    Nicole King

    Article

    Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

    Insert

    Sro5spag6_mut-nanoluc-Sro3EFL/Sro5actin-firefly-Sro3actin
    

    Use

    Luciferase

    Mutation

    N/A (synthetic)

    Available Since

    Aug. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP

    Plasmid

    #188272

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    AaCas12b(Q119F/E475R/E758R)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-PlmCasX(T26R/K610R/K808R)-T2A-EGFP

    Plasmid

    #188274

    Purpose

    Mammalian expression, Genome editing

    Depositor

    Wei Li

    Article

    Chen et al Innovation (Camb). 2022 May 26;3(4):1002

    Insert

    PlmCasX(T26R/K610R/K808R)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPG-GPD-TRPC

    Plasmid

    #190113

    Purpose

    Expression vector for fungal (Aspergillus flavus) genes based on Aspergillus parasiticus pyrG gene selection

    Depositor

    Perng-Kuang Chang

    Article

    Chang et al Fungal Genet Biol. 2017 Jul;104:29-37. d

    Type

    Empty backbone

    Expression

    Yeast

    Available Since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pILGFP4D41

    Plasmid

    #185841

    Purpose

    Testing the CYC1 core promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporter

    Depositor

    Claudia Vickers

    Article

    Peng et al Commun Biol. 2022 Feb 16;5(1):135. doi:

    Insert

    PURA3>KlURA3>TAgTEF1-PCYC1+[Z268]>yEGFP>TPGK1-TURA3
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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